PRELIMINARY OBSERVATIONS OF THE EFFECTS OF LORAZEPAM ON THE DEVELOPMENT OF CALLIPHORA VICINA AND CALLIPHORA LOEWI (DIPTERA: CALLIPHORIDAE) AND PMI ESTIMATION

Year 2014, Volume: 3 Issue: 2, 45 - 52, 05.05.2015

Ferhat Altunsoy , Furkan Akay Cenk Önsoy

https://doi.org/10.18036/btdc.42113

Abstract

Larvae of Calliphora vicina and Calliphora loewi were reared on minced cattle lung tissues treated with different Lorazepam concentrations based on levels of public common usage for therapeutical, narcotic and suicidal purposes to evaluate the reliability of insect larvae for toxicological investigations. The species are studied to determine time deviations caused by toxicological influences owing to their features like speed on heading the corpse, value on PMI estimation, briefness at developmental processes and easiness about rearing at laboratory conditions. Body length, weight, death ratio of larvae and pupa were compared within control groups. The results demonstrated that larvae feeding on drug containing tissues in slight dosages developed more rapidly and adult emergence was greater, but also higher concentrations created completely adverse results. The time required for pupation was significantly greater for colonies fed on tissues from lorazepam-dosed diets than for the control group. Results emphasize the importance of determining the contamination rates of lorazepam are essential to prevent deviation on PMI estimations

Keywords

Forensic Entomology, Lorazepam, Calliphoridae, Larval growth rate, Postmortem interval, Benzodiazepine, Entomotoxicology.

ANADOLU ÜNİVERSİTESİ

Abstract

Halk arasında tedavi amaçlı, narkoz etkisi veya intihar amaçlı olarak kullanılan Lorazepam farklı konsantrasyonlarda kıyılmış koyun karaciğerinde yetiştirilen Calliphora vicina ve Calliphora loewi türlerinin besin ortamlarına eklenerek larvalar üzerindeki etkileri toksik araştırma amaçlı olarak incelenmiştir.Bu türler cesede ilk erişen örnekler oldukları için PMI hesaplamasında ve ölüm hakkında detaylı bilgi verebilmesi açısından ve labaratuvar ortamında rahatlıkla kültürlerinin kurulabilmesi yönünden çokça çalışılan türlerdir. Pupa ve larvaların vücut uzunlukları, ağırlıkları ve ölüm oranları kontrol gruplarıyla karşılaştırılmıştır. Sonuçlar göstermektedir ki düşük dozlarda Lorazepam etkisi larvalarda gelişimi artırmakta ve ergin evreye geçişi hızlandırmakta iken yüksek dozlara geçildikçe larvalarda ve pupalarda bozulmalar ve ölüm oranları hızla artmaktadır. Sonuç olarak PMI hesaplamasında ki sapmaların önüne geçebilmek için toksik etkinin miktarının belirlenmesinin önemi vurgulanmaktadır

Keywords

Adli entomoloji, Lorezepam, Calliphoridae, Larval gelişme oranı, Ölüm zamanı hesaplaması, Benzodiazepin, Entomotoksikoloji

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