EFFECT OF EXOGENOUSLY APPLIED SPERMINE AND PUTRESCINE ON GERMINATION AND IN VITRO GROWTH OF PEPPER (CAPSICUM ANNUUM L.) SEEDS UNDER SALT STRESS

Year 2014, Volume: 3 Issue: 2, 63 - 71, 05.05.2015

Esra Koç , Ayşen Üstün , Cemil İşlek Yeliz Kaşko Arıcı

https://doi.org/10.18036/btdc.61431

Cited By: 2

Abstract

Plant growth regulators play a significant role in germination of seed. In this study,  Effects of exogenous polyamines (spermine and putrescine) on germination of seed and seedling growth such as radicula length, hypocotile length, fresh weight, dry weight of pepper under salt stress were investigated. According to results, increased salt concentration resulted in a significant reduction in germination (P<0.05). NaCl prevented hypocotile growth and this inhibition was reversed by applying spermine. During application of 2 mM spermine + 200 mM NaCl (P<0.05),  the best germination was recorded. On the contrary, germination of seed was inhibited by single application of Put but hypocotile and radicula length of seedling were increased seriously by application of 0.01 mM putrescine + 200mM NaCl and 1mM putrescine + 50 mM NaCl (P<0.05) in pepper plant.

 

Keywords

Germination, Growth, Pepper, Putrescine, Polyamines, Spermine

ANADOLU ÜNİVERSİTESİ

Abstract

Bitki büyüme düzenleyicileri tohum çimlenmesinde önemli bir rol oynar. Bu çalışmada tuz stresi altında biberin fide büyümesi (radikula uzunluğu, hipkotil uzunluğu, taze ağırlık, kuru ağırlık) ve tohum çimlenmesi üzerine dışsal poliaminler (spermin ve putresin)’in etkisi araştırılmıştır. Artan tuz konsantrasyonu çimlenme üzerinde önemli bir azalmaya neden olmuştur (P<0.05). NaCl hipokotil büyümesini inhibe etmiştir ve bu inhibisyon spermin uygulaması ile engellenmiştir. En iyi çimlenme 2 mM spermin + 200 mM NaCl uygulamasında belirlenmiştir (P<0.05). Tek başına Put uygulması çimlenmeyi inhibe etmiştir, fakat hipokotil ve radikula uzunluğu 0.01 mM putresin + 200mM NaCl and 1mM putresin + 50 mM NaCl uygulamaları ile önemli bir şekilde artmıştır (P<0.05)

Keywords

Çimlenme, Büyüme, Biber, Putresin, Poliaminler, Spermin

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