UTILIZATION OF ANTHER CULTURE FOR SCREENING OF DOUBLED HAPLOID LINES IN SOME TURKISH BREAD WHEAT HYBRIDS

Year 2014, Volume: 3 Issue: 2, 53 - 61, 05.05.2015

Ozcan Yorgancılar Aysel Yorgancılar Aysen Yumurtacu

https://doi.org/10.18036/btdc.81498

Abstract

In the current research, it is aimed to investigate the doubled haploid (DH) productivity of 30 F2 derived wheat crosses constructed after hybridization of two Turkish bread wheat donors with different bread wheat genotypes, Sonmez01 and Es26. Especially, donor parents carrying out high yield performance and disease resistance selected from new developed bread wheat genotypes. Overall plant material sowed to the Transitional Zone Agricultural Research Institute field on 2010-2011 season, and an average of 40 spikes at suitable development stage harvested from each group. Under anther culture conditions, MN6 used as initial culture media and modified 190-II choosed as regeneration media. Callus obtained from two thirds of F2 hybrids and they generated 16 albino plantlets. Extended ratios of callus production ranged between 3.25% and 50.5%. However green plantlet regeneration percentage calculated as 6.25% only in one hybrid, seven hybrid wheat samples generated only 0.25% green plantlet. According to overall results, five new DH wheat formed from different F2 hybrids derived from Sonmez01, while there were any dihaploid plants observed in F2 hybrids derived from Es26. In the sum, genome and phenome based evaluation of these F2 derived five doubled haploid bread wheat hybrids can help to describe an extra population source for next generation breeding platforms that they designed to extract new resistant crops against to environmental stresses. Also, modified anther culture method used in this study might be employed for screening of other bread wheat cultivars, lines and registered genotypes in the future DH wheat population construction

Keywords

anther; albino; bread wheat; callus; doubled haploid; hybrid

BAZI TÜRK EKMEKLİK BUĞDAY MELEZLERİNDE KATLANMIŞ HAPLOİD HATLARIN TARANMASI İÇİN ANTER KÜLTÜRÜNÜN KULLANIMI

Abstract

Mevcut çalışmada, iki Türk ekmeklik buğday çeşidinin (Sönmez01 ve Es26) farklı ekmeklik buğdaylarla melezlenmesi sonrası oluşturulmuş 30 F kökenli buğday çaprazının katlanmış haploid (DH) verimliliğini araştırmak amaçlanmıştır. Özellikle verici ebeveynler yüksek verimli ve hastalıklara dirençli yeni geliştirilmiş ekmeklik buğday genotiplerinden seçilmiştir. Tüm bitki materyali 2010-2011 sezonunda Geçit Kuşağı Tarımsal Araştırma Enstitüsü arazisine ekilmiş ve her gruptan gelişimin uygun evresinde ortalama 40 başak hasat edilmiştir. Anter kültürü koşulları altında, MN6 başlangıç besiyeri olarak kullanılmış, 190-II rejenerasyon besiyeri olarak seçilmiştir. F2 melezlerinin üçte ikisinden kallus elde edilmiş ve bunlar 16 albino bitkicik üretmişlerdir. Kallus üretiminin genişletilmiş oranları %3.25 ile %50.5 arasında değişmiştir. Yedi melez buğday örneği sadece %0.25 yeşil bitkicik üretmiş, ancak sadece bir melezde yeşil bitkicik rejenerasyon yüzdesi %6.25 olarak hesaplanmıştır. Es26’dan kökenlenmiş F2 melezlerinde hiç katlanmış haploid bitki gözlenmemişken, tüm sonuçlara göre, beş yeni katlanmış haploid (DH) buğday Sönmez01’den kökenlenmiş farklı F2 melezlerinden oluşmuştur. Özetle, F kökenli bu beş katlanmış haploid ekmeklik buğday melezinin genom ve fenom temelli değerlendirilmesi, çevresel streslere karşı dayanıklı yeni bitki seçmede yeni nesil ıslah platformları için dizayn edilmiş ilave bir populasyon kaynağı tanımlamaya yardımcı olabilir. Ayrıca, bu çalışmada kullanılmış modifiye anter kültürü metodu, gelecekte diğer ekmeklik buğday çeşitleri, hatları ve tescilli genotipler için katlanmış haploid (DH) ekmeklik buğday populasyonu oluşturulmasında kullanılabilir.

Keywords

Anther kültürü, Albino, Ekmeklik buğday, Kallus, Katlanmış haploid, Hibrid

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