Morphological Characteristics and Molecular Analysis of Newly Recorded Longidorus moesicus Lamberti, Choleva & Agostinelli, 1983 (Dorylaimida: Longidoridae) From Türkiye

Year 2023, , 187 - 198, 31.12.2023

Serkan Çelik , Faruk Akyazı

https://doi.org/10.29278/azd.1333478

Abstract

Objective: This study was conducted to determine the morphological, morphometric, and molecular characteristics of Longidorus moesicus, which was first recorded in Türkiye.
Materials and Methods: A nematode survey was conducted in rose cultivation greenhouses in 2017 in Yalova province, Türkiye. Nematodes were extracted from soil samples using Cobb's sieve method and centrifugal flotation technique. Morphological characters and morphometric measurements were used to identify the obtained nematodes. Additionally, identification was confirmed by molecular analyses of the 28S rRNA gene D2/D3 expansion region sequences.
Results: Needle nematodes in the genus Longidorus (Micoletzky, 1922) include many ectoparasitic nematode species that transmit nepoviruses as well as causing damage by direct feeding on root cells. A Longidorus species was found in the examined soils.
Conclusion: To our knowledge, this is the first report of Longidorus moesicus Lamberti, Choleva & Agostinelli, 1983 in Türkiye.

Keywords

Needle nematodes, Longidorus spp., Longidorus moesicus, Rosa spp.

Supporting Institution

Ordu Üniversitesi Bilimsel Araştırmalar Birimi (ODUBAP)

Project Number

BY-1713

Thanks

We thank Ordu University for their support to this project (ODUBAP; Project No, BY-1713)

Türkiye'den Yeni Bir Kayıt Longidorus moesicus Lamberti, Choleva & Agostinelli, 1983 (Dorylaimida: Longidoridae)'un Morfolojik Özellikleri ve Moleküler Analizi

Abstract

Amaç: Bu çalışma, Türkiye’de ilk kayıt olan Longidorus moesicus’un morfolojik, morfometrik ve moleküler karakterlerlerinin belirlenmesi amacıyla yapılmıştır.
Matertal ve Yöntem: Yalova ili gül yetiştirilen seralarda 2017 yılında nematod sürveyi gerçekleştirilmiştir. Nematodlar, santrifüj ve Cobb elek yönteminin bir modifikasyonu ile topraktan ekstrakte edilmiştir. Elde edilen nematodların teşhisinde morfolojik karakterler ve morfometrik ölçümler kullanılmıştır. Teşhisler ayrıca 28S rRNA geni D2/D3 genleşme bölgesi dizilerinin moleküler analizleri ile doğrulanmıştır.
Araştırma Bulguları: Longidorus (Micoletzky, 1922) cinsi içerisinde yeralan iğne nematodları, bitki hücreleri ile doğrudan beslenerek zarar vermesinin yanısıra nepovirüsleri ileten çok sayıda ektoparazitik nematod türlerini içermektedir. İncelenen topraklarda Longidorus cinsi bir nematod türü tespit edilmiştir.
Sonuç: Bildiğimiz kadarıyla Longidorus moesicus Lamberti, Choleva & Agostinelli, 1983'ün Türkiye için ilk rapordur.

Keywords

İğne nematodları, Longidorus spp., Longidorus moesicus, Rosa spp.

Project Number

BY-1713

References

• Archidona-Yuste, A., Cantalapiedra-Navarrete, C., Castillo, P., & Palomares-Rius, J. E. (2019). Molecular phylogenetic analysis and comparative morphology reveals the diversity and distribution of needle nematodes of the genus Longidorus (Dorylaimida: Longidoridae) from Spain. Contributions to Zoology, 88(1), 1-41.
• Arinç, Y. (1982). Ege Bölgesi Bağ Alanlarında Zararlı Olan Xiphinema Türleri (Nematoda: Longidoridae), Yayılışı, Konukcuları ve Zararları Üzerinde Araştırmalar. Izmir Böl. Zir. Müc. Aras. Ens. Arastìrma Eserleri Serisi, 41, 83s.
• Aydeniz, M. M., Özdemir, F. G. G., & Yaşar, B. (2018). Manisa ili Salihli ilçesinde meyve bahçelerinde bulunan bitki paraziti nematodların belirlenmesi. 6th ASM International Congress of Agriculture and Environment, 2018, Isparta, Türkiye (in Turkish with abstract in English), 68-75.
• Byrd, D. W., JR., Nusbaum, C. J. & Barker, K. R. (1966). A rapid flotation-sieving technique for extracting nematodes from soil. Plant Disease Reporter, 50, 954-957.
• Barsi, L., & Lamberti, F. (2004). Morphometrics of Longidorus juvenilis and first record of L. aetnaeus and L. moesicus (Nematoda: Dorylaimida) from Serbia. Nematologia mediterranea, 32(1), 5-14.
• Buser, A. 1990. Untersuchungen über die Pfeffingerkrankheit der Süsskirsche und deren Véklor Longidorus macrosoma. Eidgn. Techn. Hochschule Zürich, Switzerland, Dissertation No 9194, 205p.
• Cai, R., Prior, T., Lawson, B., Cantalapiedra-Navarrete, C., Juan E. Palomares-Rius, Castillo, P. & Archidona-Yuste, A. 2020. An integrative taxonomic study of the needle nematode complex Longidorus goodeyi Hooper, 1961 (Nematoda: Longidoridae) with description of a new species. European journal of Plant Pahology, (158), 59-81.
• Castillo, P., Vovlas, N., Subbotin, S., & Troccoli, A. (2003). A new root-knot nematode, Meloidogyne baetica n. sp.(Nematoda: Heteroderidae), parasitizing wild olive in Southern Spain. Phytopathology, 93(9), 1093-1102. Cobb, N. A. (1918). Agricultural technology circular. Washington, DC: US Department of Agriculture, Bureau of Plant Industry, (1), 48.
• Coiro, M. I., Agostinelli, A., & Lamberti, F. (1992). I Longidoridae (Nematoda) nei vigneti della provincia di Verona. Nematologia Mediterranea, 20, 87-95.
• Decraemer, W., & Robbins, R. T. (2007). The who, what and where of Longidoridae and Trichodoridae. Journal of Nematology, 39(4), 295.
• Eisenback, J. (1985). Diagnostic characters useful in the identification of the four most common species of rooot-knot nematodes (Meloidogyne spp). En:Bartker, K.; Carter, C. y Sasser, J. (Ed). An advanced treatise on Meloidogyne. Vol. II, Methodology.North Carolina State University Graphics, p.95-112.
• Elekcioglu, I. H. (1992). Untersuchungen Zum Auftreten und Zur Verbreitung Phytoparasitarer Nematogen in den Land Wirtschafflichen Hauptkulturen des Astmediterranen Gebretes der Turkei. Plits, 10(5): 120 (in German with abstract in English).
• Gutiérrez-Gutiérrez, C., Santos, M. T., Inácio, M. L., Eisenback, J. D., & Mota, M. (2020). Description of Longidorus bordonensis sp. nov. from Portugal, with systematics and molecular phylogeny of the genus (Nematoda, Longidoridae), Zoosystematics and Evolution, 96(1): 175-193.
• Harrison, B. D. (1964). Specific nematode vectors for serologically distinctive forms of raspberry ringspot and tomato black ring viruses. Virology, 22(4), 544-550.
• He, Y., Subbotin, S. A., Rubtsova, T. V., Lamberti, F., Brown, D. J., & Moens, M. (2005). A molecular phylogenetic approach to Longidoridae (Nematoda: Dorylaimida). Nematology, 7(1), 111-124.
• Hooper, D. J. (1961). A redescription of Longidorus elongatus (de Man, 1876) Thome & Swanger, 1936 (Nematoda, Dorylaimidae) and descriptions of five new species of Longidona from Great Britain. Nematologica, 6(3), 237-257.
• Kepenekci, İ., Evlice, E., & Toktay, H. (2006). Plant parasitic nematodes associated with Vineyards (Vitis vinifera L.) in the Central anatolia region of Turkey. In 28th Europen Society of Nematologist Congress, Blagoevgrad, Sofia, Bulgaria, Pensoft Publishers. Abstract, 156 p.
• Kumar, S., Stecher, G., & Tamura, K. (2016). MEGA7: molecular evolutionary genetics analysis version 7.0 for bigger datasets. Molecular biology and evolution, 33(7), 1870-1874.
• Lamberti, F. & Roca, F. (1987). Present status of nematodes vectors of plant viruses. In: Veech, J. A., Dickson, D.W. (Eds). Vistas on Nematology. Florida, De Leon Springs, pp. 321-328.
• Lamberti, F., Choleva, B., & Agostinelli, A. (1983). Longidoridae from Bulgaria (Nematoda, Dorylaimida) with description of three new species of Longidorus and two new species of Xiphinema. Nematologia mediterranea, 11(1): 49-72.
• Micoletzky, H., (1922). Die freilebenden Erd-Nematoden mit besonderer Berücksichtigung der Steiermark und der Bukowina, zugleich mit einer Revision sämtlicher nichtmariner, freilebender Nematoden in Form von GenusBeschreibungen und Bestimmungsschlusseln. Archiv für Naturgeschichte 87(9), 321–650.
• Norton, C. D., & Hoffmann, J. K. (1975). Longidorus breviannulatus n. sp.(Nematoda: Longidoridae) associated with stunted corn in Iowa. Journal of Nematology, 7(2), 168-171.
• Öztürk, G., & Enneli, S. (1994). Distribution of plant parasitic nematodes in alfa growing areas in Central Anatolia Region of Turkey. In 9th Congress of the Mediterranean Phytopathological Union (pp. 537-538).
• Öztürk, L., Avcı, G. G., Behmand, T. & Elekcioğlu, H. I. (2017). Incidence of viruses and vector nematodes in Thrace vineyards, Turkey. International Journal of Agriculture and Environmental Research, 3 (6): 4078- 4089.
• Pagan, C., Coyne, D., Carneiro, R., Kariuki, G., Luambano, N., Affokpon, A., & Williamson, V. M. (2015). Mitochondrial haplotype-based identification of ethanol-preserved root-knot nematodes from Africa. Phytopathology, 105(3), 350-357.
• Palomares-Rius, J. E., Subbotin, S. A., Landa, B. B., Vovlas, N., & Castillo, P. (2008). Description and molecular characterisation of Paralongidorus litoralis sp. n. and P. paramaximus Heyns, 1965 (Nematoda: Longidoridae) from Spain. Nematology, 10(1), 87-101.
• Robbins, R. T., & Brown, D. J. F. (1996). Descriptions of three new Longidorus species from Alaska (Nematoda: Longidoridae). Journal of nematology, 28(1), 83-93.
• Seinhorst, J. W. (1959). A rapid method for the transfer of nematodes from fixative to anhydrous glycerin. Nematologica, 4(1), 67-69.
• Širca, S., & Urek, G. (2009). Morphological and molecular characterization of six Longidorus species (Nematoda: Longidoridae) from Slovenia. Russian Journal of Nematology, 17(2), 95.
• Tamura, K., & Nei, M. (1993). Estimation of the number of nucleotide substitutions in the control region of mitochondrial DNA in humans and chimpanzees. Molecular biology and evolution, 10(3), 512-526.
• Taylor, C. E., & Brown, D. J. F. (1997). Nematode vectors of plant viruses (CAB International). Wallingford, UK. pp. 286.
• Taylor, G. E. (1962). Transmission of raspberry ringspot virus by Longidorus elongatus (de Man)(Nematoda: Dorylaimidae). Virology, 17(3), 493-494.
• Tzortzakakis, E. A., Archidona-Yuste, A., Cantalapiedra-Navarrete, C., Nasiou, E., Lazanaki, M. S., Kabourakis, E. M., Palomares-Rius, J. E. & Castillo, P. (2014). Integrative diagnosis and molecular phylogeny of dagger and needle nematodes of olives and grapevines in the island of Crete, Greece, with description of Xiphinema cretense n. sp. (Nematoda, Longidoridae). European Journal of Plant Pathology, 140 (3), 563-590.
• Tzortzakakis, E. A., Clavero-Camacho, I., Cantalapiedra-Navarrete, C., Ralli, P., Palomares-Rius, J. E., Castillo, P., & Archidona-Yuste, A. (2021). Morphological and molecular characterisation of (Nematoda: Longidoridae), first report from Greece. Journal of Nematology, 53(1), 1-10.
• Waele, D. D., & Coomans, A. (1990). Occurrence and ecology of longidorid nematodes in Belgium. Revue de nématologie, 13(2), 197-202.
• Xu, Y., Guo, K., Ye, W., Wang, J., Zheng, J., & Zhao, Z. (2017). Morphological and molecular characterisation of Longidorus juglans sp. nov. and a sister species L. fangi Xu & Cheng, 1991 (Nematoda: Longidoridae) from China. Nematology, 19(8), 951-970.
• Ye, W., & Robbins, R. T. (2004). Cluster analysis of Longidorus species (Nematoda: Longidoridae), a new approach in species identification. Journal of Nematology, 36(3), 207-218.