Does diabetes affect the carbohydrate secretions of the endometrial glands of rats in early pregnancy?

Year 2020, Volume: 9 Issue: 3, 149 - 156, 23.12.2020

Feyza Başak , Tolunay Kozlu

Abstract

Endometrial gland secretions play an important role in uterine receptivity, and further implantation they provide nourishment for the embryo. Diabetes is known to alter the secretions of certain cells. This study aimed to identify the carbohydrate content of endometrial gland secretions using Peanut Aglutinin (PNA), Wheat Germ Agglutinin (WGA) Concanavalin-A (Con-A), Soy Bean Agglutinin (SBA). Thirty-two Wistar Albino rats were divided into 4 groups of 8: diabetes and pregnancy positive, diabetes positive, pregnancy positive, control group. Samples were collected on the 5th and 7th day of pregnancy, and in the vaginally stimulated non-pregnant rats.
Positive PNA staining occurred in both groups with diabetes and was negative in the other two groups. Diabetes produced marked alterations in SBA staining in the early days of pregnancy, but did not persist for long. With regard to WGA, when diabetes combined with pregnancy, changes in the secretion of the endometrial glands occur. Con- A staining showed that both pregnancy and diabetes affect the amounts of the α- mannose chain, α- chain glucose, and N-acetyl glucosamine α- chain components of endometrial gland secretions. In conclusion this study shows that both diabetes and pregnancy affect the carbohydrate content of the secretions from the endometrial glands.

Keywords

diabetes, endometrial gland, lectin histochemistry, rat, streptozotocin

Supporting Institution

Mustafa Kemal University Scientific Research Project Coordination Unit

Project Number

9761

Does Diabetes Affect the Carbohydrate Secretions of the Endometrial Glands of Rats in Early Pregnancy?

Abstract

Endometriyal bez salgıları uterus alıcılığında önemli bir rol oynar ve implantasyondan önce embriyonun beslenmesini sağlar. Diyabetin belirli hücrelerin salgılarını değiştirdiği bilinmektedir. Bu çalışmada, Fıstık Aglutinin (PNA), Buğday Germ Aglütinin (WGA) Concanavalin-A (Con-A), Soya Fasulyesi Agglutinin (SBA) kullanılarak endometriyal bez salgılarının karbonhidrat içeriği tespit edilmiştir. Otuz iki Wistar Albino rat, her birinde 8 adet rat bulunan 4 gruba ayrıldı: diyabet ve gebelik pozitif, diyabet pozitif, gebelik pozitif, kontrol grubu. Numuneler gebeliğin 5. ve 7. günlerinde ve gebelik olmayan gruplarda da vajinal olarak stimule edilmiş sıçanlardan stimülasyonu izleyen 5. ve 7. günlerde toplandı.
Her iki diyabetli grupta pozitif PNA boyaması meydana geldi ve diğer iki grupta boyanma olmadı. Diyabet, gebeliğin ilk günlerinde SBA boyamasında belirgin değişikliklere neden oldu, ancak uzun süre devam etmedi. WGA ile ilgili olarak, diyabet gebelikle birleştiğinde, endometriyal bezlerin salgılanmasında değişiklikler meydana geldiği görüldü. Con-A lektini ile yapılan boyamalar, hem gebeliğin hem de diyabetin endometriyal bez salgılarının α-mannoz zinciri, α-zincir glikoz ve N-asetil glukozamin α-zincir bileşenlerinin miktarlarını etkilediğini göstermiştir. Sonuç olarak bu çalışma hem diyabetin hem de gebeliğin endometriyal bezlerden salgıların karbonhidrat içeriğini etkilediğini göstermektedir.

Keywords

diyabet, endometriyal bez, lektin histokimyası, rat

Project Number

9761

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