Sıçanların İçme Suyuna Bor İlavesinin Hematolojik Parametreler ile Serum Bor, Kalsiyum, Fosfor ve Magnezyum Seviyelerine Etkilerinin Araştırılması

Year 2025, Volume: 14 Issue: 1, 24 - 30, 28.03.2025

Hasan Susar , Çağla Çelebi , Murat Çelebi , Okan Rüstemoğlu , Pelin Dinç , İzzet Karahan

Abstract

Amaç: Bu araştırmada; sıçanların içme suyuna katılan borun, serum bor düzeyleri, kalsiyum, magnezyum ve fosfor konsantrasyonlarına etkisi ve hematolojik parametrelerde oluşturabileceği değişikliklerin belirlenmesi amaçlanmıştır. Gereç ve Yöntem: Araştırmada, 6 adet kontrol, 6 adet deney grubu olmak üzere toplam 12 adet 250-350 gr Wistar Albino erkek sıçan kullanıldı. Ad libitum olarak beslenen sıçanların içme suyuna, 2 mg/gün/sıçan/25 ml boraks dekahidrat (Sigma) ilave edildi. 14 günün sonunda sıçanlar dekapite edildi. ICP-OES ile serum bor analizi Tokay ve Bagdat'a (2022) göre yapılmıştır. Ayrıca; Ca, Mg, P konsantrasyonları biyokimyasal analizör cihazı ile belirlendi. Bulgular: Sıçanların serum bor düzeyi incelendiğinde deney grubu hayvanların seviyesi kontrol grubuna göre anlamlı bir şekilde yüksek (p:0.0107) bulundu. Ancak, hematolojik parametreler ile serum kalsiyum, magnezyum ve fosfor seviyeleri arasında anlamlı bir ilişki olmadığı tespit edildi. Sonuç: Bu çalışmanın sonucunda, sıçanlara içme suyu ile verilen borun, incelenen minerallerin üzerine etkisinin olmadığı tespit edildi. Ancak, bu konu hakkında yapılacak daha kapsamlı çalışmalarla daha fazla bilgi sahibi olunabileceği sonucuna varıldı.

Keywords

Bor, İçme Suyu, Rat.

Project Number

2209-A Project No: 1919B012325519

Investigation of the Effects of Boron Addition to Drinking Water of Rats on Hematologic Parameters and Serum Boron, Calcium, Phosphorus and Magnesium Levels

Abstract

Objective: In this study, it was aimed to determine the effect of boron added to the drinking water of rats on serum boron levels, calcium, magnesium and phosphorus concentrations and the changes that may occur in hematological parameters. Materials and Methods: A total of 12 250-350 g Wistar Albino male rats, 6 in the control group and 6 in the experimental group, were used in the study. The rats were fed ad libitum and 2 mg/day/rat/25 ml borax decahydrate (Sigma) was added to the drinking water. At the end of 14 days, the rats were decapitated. Serum boron analysis by ICP-OES was performed according to Tokay and Bagdat (2022). In addition; Ca, Mg, P concentrations were determined with a biochemical analyzer device. Results: When the serum boron levels of the rats were analysed, those in the experimental group were significantly higher (p:0.0107) than those in the control group. However, there was no effect on haematological parameters or serum calcium, magnesium and phosphorus levels. Conclusion: As a result of this study, it was determined that boron given to rats with drinking water had no effect on the minerals examined. However, it was concluded that more information could be obtained with more comprehensive studies on this subject.

Keywords

Boron, Drinking Water, Rat.

Ethical Statement

This study was carried out with the permission of the Animal Experiments Local Ethics Committee of Balıkesir University (BAUN-HADYEK) (permission no. 2024/11 of November 28, 2024).

Supporting Institution

The Scientific and Technological Research Council of Türkiye

Project Number

2209-A Project No: 1919B012325519

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