Kefirin sıçanlarda siklofosfamid-nedenli oksidatif stres ve akciğer hasarı üzerine antioksidan ve koruyucu etkilerinin in vitro olarak değerlendirilmesi

Year 2024, Volume: 5 Issue: 1, 11 - 18, 30.04.2024

Songül Çetik Yıldız , Cemil Demir , Mustafa Cengiz , Betül Peker Cengiz , Adnan Ayhancı

https://doi.org/10.58605/bingolsaglik.1436057

Abstract

Amaç: Doğal bir probiyotik olan kefir, antioksidatif ve antitümör özelliklerinin yanı sıra mikrobiyal türler ve fermantasyondan kaynaklanan yararlı özelliklere de sahiptir. Siklofosfamid (SF), kanser tedavisinde oldukça tercih edilen ancak hem humoral hem de hücresel bağışıklığı baskılayabilen sitotoksik bir ilaçtır. SF'nin immünsüpresif etkisinin olması ve akciğer gibi çoklu organ toksisitesine neden olması nedeniyle etkili yüksek dozda kullanımı sınırlıdır.
Yöntem: Wistar albino sıçanlar 6 gruba ayrıldı. Kontrol, 150 mg/kg SF, 5 mg/kg kefir, 5 mg/kg kefir +150 SF, 10 mg/kg kefir, 10 mg/kg kefir+150 SF. Ratlara 1., 2. ve 3. günlerdeki fermente kefirler karıştırılarak 12 gün süreyle verilirken, deneyin 12. gününde SF tek doz ve i.p. olarak verildi. Alınan kan ve doku parametreleri değerlendirildi.
Bulgular: Oksidatif stresi gösteren OSI değerinin SF uygulanan grupta arttığı, SF+kefir gruplarında ise bu düzeyin kontrole yaklaştığı görüldü. Ayrıca SF uygulanan sıçanların akciğer parankiminde dejenerasyon, interalveoler bölgede yoğun inflamasyon, alveollerde kollaps ve bronşiyol çevresinde yoğun inflamasyon gözlenirken, kefir verilen gruplarda bu etkilerin etkin bir şekilde düzeldiği gözlendi.
Sonuç: Sonuç olarak deneysel çalışmamızda kefirin SF kaynaklı oksidatif stres, konjesyon, alveolar hasar ve inflamasyon üzerinde antioksidatif ve koruyucu etkiler gösterdiği gözlendi.

Keywords

Kefir, siklofosfamid, antioksidan, oksidatif stres, akciğer hasarı, sıçan

Ethical Statement

This study was approved by Ethics Committee of Eskisehir Osmangazi University Animal Experiments Local Ethics Committee (784-145 / 2020).

Supporting Institution

This study was financed by Mardin Artuklu Univ. BAP Coordination Office (MAU.BAP.20.SHMYO.004).

Project Number

MAU.BAP.20.SHMYO.004

Evaluation of in vitro antioxidative and protective effects of kefir on cyclophosphamide-upon oxidative stress and lung damage in rats

Abstract

Purpose: Kefir, a natural probiotic, has antioxidative and antitumor properties, as well as potential beneficial properties resulting from microbial species and fermentation. Cyclophosphamide (CYP) is a cytotoxic drug that is highly preferred in cancer therapy but can suppress both humoral and cellular immunity. The use of effective doses is limited because CYP has an immunosuppressive effect and causes multiple organ toxicity such as the lungs, and the use of effective high doses is restricted.
Methods: Wistar albino rats were divided into 6 groups. As; control, 150 mg/kg CYP, 5 mg/kg kefir, 5 mg/kg kefir +150 CYP, l0 mg/kg kefir, l0 mg/kg kefir+150 CYP. Fermented kefirs from the 1st, 2nd and 3rd days were mixed and given to the rats for 12 days, while CYP was given as a single dose and ip on the 12th day of the experiment. The received parameters were evaluated.
Results: It was observed that the OSI value, which indicates oxidative stress, increased in the CYP-administered group, and this level approached control in the CYP+kefir groups. In addition, while degeneration, intense inflammation in the interalveolar area, collapse of the alveoli, and intense inflammation around the bronchioles were observed in the lung parenchyma of CYP-administered rats, it was observed that these effects were effectively improved in the groups given kefir+CYP.
Conclusion: In conclusion, in our study, it was observed that kefir showed antioxidative and protective effects on CYP-induced oxidative stress, congestion, alveolar damage and inflammation.

Keywords

Kefir, cyclophosphamide, antioxidant, oxidative stress, lung damage, rats

Project Number

MAU.BAP.20.SHMYO.004

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