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Glifosat uygulanmış topraktan izole edilen bakterilerin herbisit degradasyon potansiyellerinin belirlenmesi

Year 2020, Volume: 13 Issue: 2, 102 - 108, 15.08.2020

Cemal Kurtoğlu , Faik Ceylan , Sabahattin Cömertpay , İsmail Akyol

https://doi.org/10.46309/biodicon.2020.732161

Abstract

Glifosat (N-fosfonometilglisin), kararlı karbon-fosfat (C-P) bağlı, sentetik ve seçici olmayan bir herbisittir. Topraktaki glifosat kalıntılarının çevre ve insan sağlığına zararlı etkileri olduğu bilinmekle birlikte, herbisitleri besin olarak kullanıp parçalanmasını sağlayan toprak bakterilerinden bu zararlı etkileri azaltmak için yararlanılmaktadır. Bu çalışmada, Kahramanmaraş Sütçü İmam Üniversitesi’ne ait, glifosat ile muamele edilmiş tarım arazisinden izole edilen bakterilerin tanımlanması ve bu herbisiti degrade etme potansiyellerinin belirlenmesi hedeflenmiştir. Bu amaçla kullanılacak bakteri kolonileri seri seçilimlerle elde edilmiş ve gen bölgesi sekanslaması ve/veya proteine dayalı tanımlama yapan MALDI-TOF (Matrix Assisted Laser Desorption and Ionization Time-Of-Flight ) yöntemleri kullanılarak elde edilen bakterilerin tür tanımlanması yapılmıştır. Sonrasında, bu bakteriler 0.1g/L, 0.5g/L ve 1g/L glifosat dışında başka karbon kaynağı içermeyen besiyeri içerisinde yetiştirilmiş ve büyüme hızlarının belirlenmesi için 14. günde 600 nm’de absorbansları ölçülmüştür. Ayrıca, bakterilerin glifosat degradasyon yeteneklerinin belirlenmesi maksadıyla; besiyerlerinde kalan glifosat miktarları, aynı süre sonunda 570 nm’de absorbanslarının ölçülmesiyle belirlenmiştir. Elde edilen sonuçlar; izole edilen bakterilerin hiçbirinin glifosatı degrade edemediğini, glifosatın bazı bakterilerin büyümesini olumsuz yönde etkilediğini ve glifosat içeren ortamda büyüme miktarı en yüksek olan bakterilerin Klebsiella variicola ve Klebsiella pneumoniae olduğunu ortaya koymuştur.

Keywords

glifosat toprak bakterileri moleküler tanımlama

Supporting Institution

Kahramanmaraş Sütçü İmam Üniversitesi Bilimsel Araştırma Projeleri Birimi

Project Number

2018/5-6 YLS

References

  • [1] Kitiş, Y. E., Yazır, B., Özgönen Özkaya, H. (2016). The effects of some soil herbicides on root colonization and spore number of mycorrhizal fungi Glomus intraradices. Biological Diversity and Conservation, 9(2), 1-7.
  • [2] Demirkan, H. (2009). Herbisitlere dayanıklılık konusunda dünyada yapılmış bildirimlerin değerlendirilmesi. Ege Üniv. Ziraat Fak. Derg, 46(1), 71-77.
  • [3] Başaran, M. S., Serim, A. T. (2010). Herbisitlerin Toprakta Parçalanması. Selçuk Tarım ve Gıda Bilimleri Dergisi, 24(2), 54-61.
  • [4] Kanissery, R. (2018). Herbicide - Nutrient Interactions in Soil: A Short Review. Agricultural Research & Technology: Open Access Journal, 15(2). doi: 10.19080/artoaj.2018.15.555951
  • [5] Li, H., Joshi, S. R., Jaisi, D. P. (2016). Degradation and isotope source tracking of glyphosate and aminomethylphosphonic acid. Journal of Agricultural and Food Chemistry, 64(3), 529-538. doi: 10.1021/acs.jafc.5b04838
  • [6] Van Stempvoort, D. R., Roy, J. W., Brown, S. J., Bickerton, G. (2014) Residues of the herbicide glyphosate in riparian groundwater in urban catchments. Chemosphere, 95, 455-463.
  • [7] Waiman, C. V., Avena, M. J., Garrido, M., Fernández Band, B., Zanini, G. P. (2012). A simple and rapid spectrophotometric method to quantify the herbicide glyphosate in aqueous media. Geoderma, 170, 154-158. doi: 10.1016/j.geoderma.2011.11.027
  • [8] Zhang, C., Hu X., Luo, J., Wu, Z., Wang, L., Li, B., Wang, Y., Sun. G. (2015). Degradation dynamics of glyphosate in different types of citrus orchard soils in China. Molecules, 20(1), 1161-1175. doi: 10.3390/molecules20011161
  • [9] Sihtmäe, M., Blinova, I., Künnis-Beres, K., Kanarbik, L., Heinlaan, M., Kahru, A. (2013) Ecotoxicological effects of different glyphosate formulations. Applied Soil Ecology, 72, 215-224. doi: 10.1016/j.apsoil.2013.07.005
  • [10] Wang, S., Seiwert, B., Kastner, M., Miltner, A., Schaffer, A., Reemtsma, T.,Yang Q, Nowak K. M. (2016). Biodegradation of glyphosate in watersediment microcosms a stable isotope co-labeling approach. Water Research, 99, 91-100. doi: 10.1016/j.watres.2016.04.041
  • [11] Niemann, L., Sieke, C., Pfeil, R., Solecki, R. (2015). A critical review of glyphosate findings in human urine samples and comparison with the exposure of operators and consumers. Journal of Consumer Protection and Food Safety, 10, 3-12. doi: 10.1007/s00003-014-0927-3.
  • [12] Campbell, A. W. (2014). Glyphosate: Its Effects on Humans. Alternative Therapies in Health and Medicine, 20(3), 9-11.
  • [13] Pesticide Residue Monitoring Program Fiscal Year 2017 Pesticide Report. (2017). USA, U.S. Food and Drug Administration.
  • [14] Richmond, M. E. (2018). Glyphosate: A review of its global use, environmental impact, and potential health effects on humans and other species. Journal of Environmental Studies and Sciences, doi: 10.1007/s13412-018-0517-2.
  • [15] Hanke, I., Wittmer, I., Bischofberger, S., Stamm, C., Singer, H. (2010). Relevance of urban glyphosate use for surface water quality. Chemosphere, 81(3),422-429. doi: 10.1016/j.chemosphere.2010.06.067
  • [16] Shushkova, T., Ermakova, I., Leontievsky, A. (2010). Glyphosate bioavailability in soil. Biodegradation, 21(3), 403-410.
  • [17] Grandcoin, A., Piel, S., Baures, E. (2017). Amino methyl phosphonic acid (AMPA) in natural waters: its sources, behavior and environmental fate. Water Research, 117, 187-197. doi: 10.1016/j.watres.2017.03.055.
  • [18] Kwiatkowska, M., Huras, B., Bukowska, B. (2014). The effect of metabolites and impurities of glyphosate on human erythrocytes (in vitro). Pesticide Biochemistry and Physsiology, 109, 34-43. doi: 10.1016/j.pestbp.2014.01.003.
  • [19] Lupi, L., Miglioranza, KS., Aparicio, V. C., Marino, D., Bedmar, F., Wunderlin, D. A. (2015). Occurrence of glyphosate and AMPA in an agricultural watershed from the southeastern region of Argentina. Science of the Total Environment, 536, 687-694. doi: 10.1016/j.scitotenv.2015.07.090
  • [20] Mercurio, P., Flores, F., Mueller, J. F., Carter, S., Negri, A. P. (2014). Glyphosate persistence in seawater. Marine Pollution Bulletin, 85(2), 385-390. doi: 10.1016/j.marpolbul.2014.01.021
  • [21] Fan, J., Yang, G., Zhao, H., Shi, G., Geng, Y., Hou, T., Tao, K. (2012). Isolation, identification and characterization of a glyphosate-degrading bacterium, Bacillus cereus CB4, from soil. Journal of General and Applied Microbiology, 58(4), 263-271. doi: 10.2323/jgam.58.263
  • [22] Firdous, S., Iqbal, S., Anwar, S. (2017). Optimization and modeling of glyphosate biodegradation by a novel Comamonas odontotermitis P2 through response surface methodology, Pedosphere, doi:10.1016/S1002-0160(17)60381-3.
  • [23] Zhao, H., Tao, K., Zhu, J., Liu, S., Gao, H., Zhou, X. (2015). Bioremediation potential of glyphosate-degrading Pseudomonas spp. Strains isolated from contaminated soil. The Journal of General and Applied Microbiology, 61, 165-170. doi: 10.2323/jgam.61.165
  • [24] Carneiro, R. T. A., Taketa, T. B., Gomes Neto R. J., Oliveira, J. L., Campos, E.V.R., Moraes, M. A., Silva, C. M. G., Beppu, M. M., Fraceto, L. F. (2015). Removal of glyphosate herbicide from water using biopolymer membranes. Journal of Environmental Management, 151, 353-360. doi: 10.1016/j.jenvman.2015.01.005
  • [25] Pipke Ping, L., Zhang, C., Zhang, C., Zhu, Y., He, H., Wu M., Tang, T., Li, Z., Zhao, H. (2014). Isolation and characterization of pyrene and benzo[a]pyrene-degrading Klebsiella pneumonia PL1 and its potential use in bioremediation. Applied Microbiology and Biotechnology, 98, 3819. doi: 10.1007/s00253-013-5469-6
  • [26] Chaudhari, A. U., Kodam, K. M. (2010). Biodegradation of thiocyanate using co-culture of Klebsiella pneumoniae and Ralstonia sp. Applied Microbiology and Biotechnology, 85, 1167-1174. doi: 10.1007/s00253-009-2299-7
  • [27] Avcioglu, N. H., Bilkay I. S. (2019). Cyanide Removal in Electroplating, Metal Plating and Gold Mining Industries Wastewaters by Using Klebsiella pneumoniae and Klebsiella oxytoca Species. European Journal of Biological Research, 78(1), 5-10.
  • [28] Kwon, G. S., Kim, J. E., Kim, T. K., Sohn, H. Y., Koh, S. C., Shin K. S., Kim, D. G. (2002). Klebsiella pneumoniae KE-1 degrades endosulfan without formation of the toxic metabolite, endosulfan sulfate. FEMS Microbiology Letters, 215, 255-259. doi: 10.1111/j.1574-6968.2002.tb11399.x
  • [29] Zhang, J., Liang, S., Wang, X., Lu, Z.,1 Sun, P., Zhang H., Sun, F. (2019). Biodegradation of Atrazine by the Novel Klebsiella variicola Strain FH-1. Hindawi BioMed Research International, 4, 1-12. doi: 10.1155/2019/4756579
  • [30] Eslami, H., Shariatifar. A., Rafiee, E., Shiranian, M., Salehi. F., Hosseini S. S., Eslami. G., Ghanbari. R., Ebrahimi, A. A. (2019). Decolorization and biodegradation of reactive Red 198 Azo dye by a new Enterococcus faecalis–Klebsiella variicola bacterial consortium isolated from textile wastewater sludge. World Journal of Microbiology and Biotechnology, 35(3), 38. doi: 10.1007/s11274-019-2608-y.
  • [31] Zhan, H., Feng, Y., Fan, X., Chen, S. (2018). Recent advances in glyphosate biodegradation. Applied Microbiology and Biotechnology, 102, 5033-5043. doi: 10.1007/s00253-018-9035-0.
  • [32] Sabullah, M.K., Rahman, M.F., Ahmad, S.A., Sulaiman, M.R., Shukor, M.S., Shamaan, N.A., Shukor, M.Y. (2016). Isolation and Characterization of A Molybdenum-Reducing and Glyphosate-Degrading Klebsiella oxytoca Strain Saw-5 in Soils from Sarawak. AGRIVITA. 38(1), 1-13. doi: 10.17503/agrivita.v38i1.654.

Year 2020, Volume: 13 Issue: 2, 102 - 108, 15.08.2020

Cemal Kurtoğlu , Faik Ceylan , Sabahattin Cömertpay , İsmail Akyol

https://doi.org/10.46309/biodicon.2020.732161

Abstract

Project Number

2018/5-6 YLS

References

  • [1] Kitiş, Y. E., Yazır, B., Özgönen Özkaya, H. (2016). The effects of some soil herbicides on root colonization and spore number of mycorrhizal fungi Glomus intraradices. Biological Diversity and Conservation, 9(2), 1-7.
  • [2] Demirkan, H. (2009). Herbisitlere dayanıklılık konusunda dünyada yapılmış bildirimlerin değerlendirilmesi. Ege Üniv. Ziraat Fak. Derg, 46(1), 71-77.
  • [3] Başaran, M. S., Serim, A. T. (2010). Herbisitlerin Toprakta Parçalanması. Selçuk Tarım ve Gıda Bilimleri Dergisi, 24(2), 54-61.
  • [4] Kanissery, R. (2018). Herbicide - Nutrient Interactions in Soil: A Short Review. Agricultural Research & Technology: Open Access Journal, 15(2). doi: 10.19080/artoaj.2018.15.555951
  • [5] Li, H., Joshi, S. R., Jaisi, D. P. (2016). Degradation and isotope source tracking of glyphosate and aminomethylphosphonic acid. Journal of Agricultural and Food Chemistry, 64(3), 529-538. doi: 10.1021/acs.jafc.5b04838
  • [6] Van Stempvoort, D. R., Roy, J. W., Brown, S. J., Bickerton, G. (2014) Residues of the herbicide glyphosate in riparian groundwater in urban catchments. Chemosphere, 95, 455-463.
  • [7] Waiman, C. V., Avena, M. J., Garrido, M., Fernández Band, B., Zanini, G. P. (2012). A simple and rapid spectrophotometric method to quantify the herbicide glyphosate in aqueous media. Geoderma, 170, 154-158. doi: 10.1016/j.geoderma.2011.11.027
  • [8] Zhang, C., Hu X., Luo, J., Wu, Z., Wang, L., Li, B., Wang, Y., Sun. G. (2015). Degradation dynamics of glyphosate in different types of citrus orchard soils in China. Molecules, 20(1), 1161-1175. doi: 10.3390/molecules20011161
  • [9] Sihtmäe, M., Blinova, I., Künnis-Beres, K., Kanarbik, L., Heinlaan, M., Kahru, A. (2013) Ecotoxicological effects of different glyphosate formulations. Applied Soil Ecology, 72, 215-224. doi: 10.1016/j.apsoil.2013.07.005
  • [10] Wang, S., Seiwert, B., Kastner, M., Miltner, A., Schaffer, A., Reemtsma, T.,Yang Q, Nowak K. M. (2016). Biodegradation of glyphosate in watersediment microcosms a stable isotope co-labeling approach. Water Research, 99, 91-100. doi: 10.1016/j.watres.2016.04.041
  • [11] Niemann, L., Sieke, C., Pfeil, R., Solecki, R. (2015). A critical review of glyphosate findings in human urine samples and comparison with the exposure of operators and consumers. Journal of Consumer Protection and Food Safety, 10, 3-12. doi: 10.1007/s00003-014-0927-3.
  • [12] Campbell, A. W. (2014). Glyphosate: Its Effects on Humans. Alternative Therapies in Health and Medicine, 20(3), 9-11.
  • [13] Pesticide Residue Monitoring Program Fiscal Year 2017 Pesticide Report. (2017). USA, U.S. Food and Drug Administration.
  • [14] Richmond, M. E. (2018). Glyphosate: A review of its global use, environmental impact, and potential health effects on humans and other species. Journal of Environmental Studies and Sciences, doi: 10.1007/s13412-018-0517-2.
  • [15] Hanke, I., Wittmer, I., Bischofberger, S., Stamm, C., Singer, H. (2010). Relevance of urban glyphosate use for surface water quality. Chemosphere, 81(3),422-429. doi: 10.1016/j.chemosphere.2010.06.067
  • [16] Shushkova, T., Ermakova, I., Leontievsky, A. (2010). Glyphosate bioavailability in soil. Biodegradation, 21(3), 403-410.
  • [17] Grandcoin, A., Piel, S., Baures, E. (2017). Amino methyl phosphonic acid (AMPA) in natural waters: its sources, behavior and environmental fate. Water Research, 117, 187-197. doi: 10.1016/j.watres.2017.03.055.
  • [18] Kwiatkowska, M., Huras, B., Bukowska, B. (2014). The effect of metabolites and impurities of glyphosate on human erythrocytes (in vitro). Pesticide Biochemistry and Physsiology, 109, 34-43. doi: 10.1016/j.pestbp.2014.01.003.
  • [19] Lupi, L., Miglioranza, KS., Aparicio, V. C., Marino, D., Bedmar, F., Wunderlin, D. A. (2015). Occurrence of glyphosate and AMPA in an agricultural watershed from the southeastern region of Argentina. Science of the Total Environment, 536, 687-694. doi: 10.1016/j.scitotenv.2015.07.090
  • [20] Mercurio, P., Flores, F., Mueller, J. F., Carter, S., Negri, A. P. (2014). Glyphosate persistence in seawater. Marine Pollution Bulletin, 85(2), 385-390. doi: 10.1016/j.marpolbul.2014.01.021
  • [21] Fan, J., Yang, G., Zhao, H., Shi, G., Geng, Y., Hou, T., Tao, K. (2012). Isolation, identification and characterization of a glyphosate-degrading bacterium, Bacillus cereus CB4, from soil. Journal of General and Applied Microbiology, 58(4), 263-271. doi: 10.2323/jgam.58.263
  • [22] Firdous, S., Iqbal, S., Anwar, S. (2017). Optimization and modeling of glyphosate biodegradation by a novel Comamonas odontotermitis P2 through response surface methodology, Pedosphere, doi:10.1016/S1002-0160(17)60381-3.
  • [23] Zhao, H., Tao, K., Zhu, J., Liu, S., Gao, H., Zhou, X. (2015). Bioremediation potential of glyphosate-degrading Pseudomonas spp. Strains isolated from contaminated soil. The Journal of General and Applied Microbiology, 61, 165-170. doi: 10.2323/jgam.61.165
  • [24] Carneiro, R. T. A., Taketa, T. B., Gomes Neto R. J., Oliveira, J. L., Campos, E.V.R., Moraes, M. A., Silva, C. M. G., Beppu, M. M., Fraceto, L. F. (2015). Removal of glyphosate herbicide from water using biopolymer membranes. Journal of Environmental Management, 151, 353-360. doi: 10.1016/j.jenvman.2015.01.005
  • [25] Pipke Ping, L., Zhang, C., Zhang, C., Zhu, Y., He, H., Wu M., Tang, T., Li, Z., Zhao, H. (2014). Isolation and characterization of pyrene and benzo[a]pyrene-degrading Klebsiella pneumonia PL1 and its potential use in bioremediation. Applied Microbiology and Biotechnology, 98, 3819. doi: 10.1007/s00253-013-5469-6
  • [26] Chaudhari, A. U., Kodam, K. M. (2010). Biodegradation of thiocyanate using co-culture of Klebsiella pneumoniae and Ralstonia sp. Applied Microbiology and Biotechnology, 85, 1167-1174. doi: 10.1007/s00253-009-2299-7
  • [27] Avcioglu, N. H., Bilkay I. S. (2019). Cyanide Removal in Electroplating, Metal Plating and Gold Mining Industries Wastewaters by Using Klebsiella pneumoniae and Klebsiella oxytoca Species. European Journal of Biological Research, 78(1), 5-10.
  • [28] Kwon, G. S., Kim, J. E., Kim, T. K., Sohn, H. Y., Koh, S. C., Shin K. S., Kim, D. G. (2002). Klebsiella pneumoniae KE-1 degrades endosulfan without formation of the toxic metabolite, endosulfan sulfate. FEMS Microbiology Letters, 215, 255-259. doi: 10.1111/j.1574-6968.2002.tb11399.x
  • [29] Zhang, J., Liang, S., Wang, X., Lu, Z.,1 Sun, P., Zhang H., Sun, F. (2019). Biodegradation of Atrazine by the Novel Klebsiella variicola Strain FH-1. Hindawi BioMed Research International, 4, 1-12. doi: 10.1155/2019/4756579
  • [30] Eslami, H., Shariatifar. A., Rafiee, E., Shiranian, M., Salehi. F., Hosseini S. S., Eslami. G., Ghanbari. R., Ebrahimi, A. A. (2019). Decolorization and biodegradation of reactive Red 198 Azo dye by a new Enterococcus faecalis–Klebsiella variicola bacterial consortium isolated from textile wastewater sludge. World Journal of Microbiology and Biotechnology, 35(3), 38. doi: 10.1007/s11274-019-2608-y.
  • [31] Zhan, H., Feng, Y., Fan, X., Chen, S. (2018). Recent advances in glyphosate biodegradation. Applied Microbiology and Biotechnology, 102, 5033-5043. doi: 10.1007/s00253-018-9035-0.
  • [32] Sabullah, M.K., Rahman, M.F., Ahmad, S.A., Sulaiman, M.R., Shukor, M.S., Shamaan, N.A., Shukor, M.Y. (2016). Isolation and Characterization of A Molybdenum-Reducing and Glyphosate-Degrading Klebsiella oxytoca Strain Saw-5 in Soils from Sarawak. AGRIVITA. 38(1), 1-13. doi: 10.17503/agrivita.v38i1.654.
There are 32 citations in total.

Details

Primary Language Turkish
Subjects Conservation and Biodiversity
Journal Section Research Articles
Authors

Cemal Kurtoğlu 0000-0001-5690-5739

Faik Ceylan 0000-0001-6740-3259

Sabahattin Cömertpay 0000-0003-4850-6927

İsmail Akyol 0000-0001-8856-0018

Project Number 2018/5-6 YLS
Publication Date August 15, 2020
Submission Date February 19, 2020
Acceptance Date May 30, 2020
Published in Issue Year 2020 Volume: 13 Issue: 2

Cite

APA Kurtoğlu, C., Ceylan, F., Cömertpay, S., Akyol, İ. (2020). Glifosat uygulanmış topraktan izole edilen bakterilerin herbisit degradasyon potansiyellerinin belirlenmesi. Biological Diversity and Conservation, 13(2), 102-108. https://doi.org/10.46309/biodicon.2020.732161
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