Seramik endüstrisi arıtma çamurundan izole edilen Aspergillus spp. ile kadmiyum ve bakır biyosorbsiyonu

Year 2019, Volume: 12 Issue: 3, 44 - 56, 15.12.2019

Elif Hasgül Semra Malkoç , Alaettin Güven , Alper Dede , Kıymet Güven

Abstract

Küfler uygun koşullar altında farklı ağır metaller için iyi bir biyosorban olabilir. Bu çalışmada, seramik sanayi
atık çamurlarından Aspergillus spp. izole edilmiş ve bakır ve kadmiyum metallerine toleransı incelenmiştir. Deneyler 25
°C’de biyosorban miktarı 2,5 g, Cu (II) için pH = 4-4,5, Cd (II) için pH = 6 ve başlangıç metal konsantrasyonu Cd (II)
için 1 mM ve Cu (II) için 5 mM olarak yapılmıştır. İki Aspergillus suşunun kadmiyum ve bakırı uzaklaştırma etkinlikleri
sırasıyla % 90-95 ve% 85-90 olarak bulunmuştur. Canlı ve ölü biyosorbanın bakır için emilim kapasiteleri sırasıyla 5,3676
mg g-1, 18,661 mg g-1 ve kadmiyum için ise sırasıyla 0,1977 mg g-1, 0,1772 mg g-1 olarak tespit edilmiştir. FTIR analizleri
bakır iyonlarının vinil bileşiklere (950-900 cm-1) ve kadmiyum iyonlarının primer amidlere (1420-1400 cm-1) bağlandığını
göstermiştir. Biyosorpsiyon sonuçları dikkate alındığında, Langmuir ve Freundlich izoterm modellerinden hiçbirinin
deneysel verilere uymadığı açıkça görülmüştür. Hücre yüzeyinin metal iyon bağlama alanları FTIR ile belirlenmiştir.
SEM izleme ve EDX analizi yapılmış, EDX sonuçları bakır ve kadmiyumun biyosorpsiyonunu doğrulamıştır.

Keywords

Aspergillus spp., kadmiyum, bakır, biyosorbsiyon, seramik endüstrisi

Biosorption of cadmium and copper by Aspergillus spp. isolated from industrial ceramic waste sludge

Abstract

Under proper conditions, fungi can act as a good biosorbent for different heavy metals. In the present study,
Aspergillus spp. have been isolated from ceramic industrial waste sludge and the tolerance of the fungi for copper and
cadmium metals were examined. The experiments were carried out at 25 °C, pH=4-4.5 for Cu(II), pH=6 for Cd(II),
biosorbent dose of 2.5 g, initial metal concentration of Cd (II) was 1 mM and Cu(II) was 5 mM. The removal efficiencies
for cadmium and copper with two Aspergillus strains were found to be 90-95% and 85-90%, respectively. The sorption
capacities of live and dead fungi for copper were 5.3676 mg g-1, 18.661 mg g-1 and for cadmium were 0.1977 mg g-1,
0.1772 mg g-1 respectively. FTIR analyses have showed that copper ions bound to vinyl compounds (950-900 cm-1) and
cadmium ions bound to primer amides (1420-1400 cm-1), mostly. Considering biosorption results, Langmuir and
Freundlich isotherm models have been described and it was clearly seen that none of the isotherm models have fitted the
experimental data. The metal ion binding areas of the cell surface of fungi were determined by FTIR. SEM monitoring
and EDX analysis were carried out. EDX results confirmed the biosorption of copper and cadmium.

Keywords

Aspergillus spp., cadmium, copper, biosorption, ceramic industry

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