In vitro androgenesis in pepper (Capsicum annuum L.) and the affecting factors on success: II. Carbohydrate source and antioxidants

Year 2021, Volume: 30 Issue: 2, 92 - 97, 15.12.2021

Ergün Doğangüzel Fatma Nur Altındağ , Merve Arefe Yiğit Şeküre Şebnem Ellialtıoğlu Nuray Çömlekçioğlu

https://doi.org/10.38042/biotechstudies.1000341

Abstract

Microspore cells develop into male gametophytes, which are released as pollen. Under
certain stress conditions, the developmental pathway of microspores can be
transformed into an embryo instead of pollen with haploid technology. In this
experiment, 2 pepper breeding lines (G-1 and G-2) and 4 nutrient media formed with
Murashige & Skoog (1962-MS) + 30 g L-1 sucrose or maltose and with or without
vitamins as antioxidants. 0.05 mg L-1 biotin (vitamin B7) and 0.5 mg L-1 ascorbic acid
(vitamin C) were studied as antioxidants. The anthers were pretreated for 2 days at 35
ºC in dark, then they were incubated in a climate chamber at 25 ºC and 16/8 hours
photoperiodic conditions. The highest embryos rate, and development of embryos
into the plantlet has been obtained from the medium containing maltose, and
antioxidants. Although there was no significant differences between genotypes in
medium- I (MS + sucrose), medium-II (MS + sucrose and antioxidants) and medium-III
(MS + maltose) in terms of embryogenic response, a significant difference was
determined between genotypes in medium-IV (MS + maltose and antioxidants). The
number of embryos obtained from the G-1 in medium-IV has increased 2.5, 6.4, and
4.5 times, compared to the medium-I, medium-II, and medium-III respectively.

Keywords

Capsicum annuum L., Anther culture, Maltose, Ascorbic acid, Biotin

Supporting Institution

The Scientific and Technological Research Council of Turkey (TÜBİTAK) and

Project Number

3170610-TÜBİTAK-TEYDEB- AGY300-03

Thanks

The United Genetics Turkey Seed Company

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