Characterization of plant growth-promoting traits and antagonistic potentials of endophytic bacteria from bean plants against Pseudomonas syringae pv. phaseolicola

Year 2019, Volume: 59 Issue: 3, 59 - 69, 30.09.2019

Kamil Duman , Soner Soylu

https://doi.org/10.16955/bitkorb.597214

Cited By: 14

Abstract

Pseudomonas syringae pv.
phaseolicola (Psp) is a
seed-borne bacterium which causes halo blight disease in common bean. This
study aimed the determination of plant growth-promoting traits (such as IAA,
phosphate solubilization) and antagonistic potentials (such as siderophore and ammonia
productions) of endophytic plant growth-promoting bacteria (PGPB) from healthy
bean plants growing in different regions against Psp in vitro conditions. A total of 131 bacteria were primarily
characterized as putative PGPB and tested for antagonist activity against Psp in dual culture tests. Seventy-one
bacterial isolates demonstrated antagonistic activities against Psp isolate in varying ratios. Among
these 71 isolates, 30 representative bacterial isolates from the different
regions/fields were selected. On the basis of morphological, physiological,
biochemical characteristics and confirmation by MALDI-TOF analyses, 30
endophytic antagonist isolates were identified as belonging to 10 genera, 24
different species. According to results obtained, 10 isolates belong to Bacillus spp., 6 isolates belong to Pseudomonas spp., 4 isolates belong to Rhizobium radiobacter, 2 isolates belong
to Arthrobacter spp., 2 isolates
belong to Achromobacter spanius, 2 isolates belong to Serratia liquefaciens, 1 isolate belongs to Acinetobacter calcoaceticus, Exiguobacterium sp., Microbacterium hydrocarbonoxydans, and Ochrobactrum anthropi. The largest and
lowest inhibition zone was produced by endophytic bacterial isolates Pseudomonas gessardii (4.85) and Bacillus licheniformis (1.35). Among the
tested antagonist bacterial isolates, 10 isolates were positive for the
production of α-amylase, 7 isolates positive for phosphate solubilization, 29
isolates positive for siderophore production, 11 isolates positive for protease
production. All selected bacterial isolates produced IAA and ammonia in
relatively varying amounts. P. gessardii
produced a relatively large amount of extracellular siderophore (5.83), Exiguobacterium sp. produced a
relatively large amount of extracellular protease (5.25), P. gessardii and O. anthropi
produced a relatively large amount of extracellular IAA (161.39 µg/ml) and Acinetobacter calcoaceticus produced a
relatively large amount of phosphatase (2.63). This is the first study reporting
bean plants harbor endophytes having plant growth promoting activities with
antagonistic potential against Psp.

Keywords

bean, halo blight, endophyte, antagonist, plant growth promoting bacteria, biological control, Pseudomonas syringae pv. phaseolicola

Supporting Institution

Tarımsal Araştırmalar ve Politikalar Genel Müdürlüğü, Mustafa Kemal Üniversitesi Fen Bilimleri Enstitüsü

Project Number

TAGEM-BS-15/09-03/02-07

Thanks

This study was supported by TAGEM under the project name TAGEM-BS-15/09-03/02-07.

Fasulye bitkisinden elde edilen endofit bakterilerin bitki gelişimini teşvik edici ve antagonistik özelliklerinin Pseudomonas syringae pv. phaseolicola etmenine karşı karekterizasyonu

Abstract

Pseudomonas
syringae pv. phaseolicola (Psp)
fasulye bitkisinde Hale yanıklığı hastalığına sebep olan tohum kökenli bir
bakteriyel hastalık etmenidir. Bu çalışmanın amacı, farklı bölgelerde yetişen
sağlıklı fasulye bitkilerinden izole edilen endofitik bitki gelişimini teşvik
eden bakterilerin (PGPB) Psp üzerine antagonistik
(siderofor ve amonyak üretimi gibi) ve bitki gelişimini teşvik eden mekanizmalarının
(IAA üretimi, fosfatı indirgeme özelliği gibi) in vitro koşullarda karakterizasyonudur. Araştırma sonunda elde
edilen 131 PGPB arasında 71 izolat Psp
etmenine karşı değişen oranlarda engelleme zonu oluşturmuş olup, bu izolatları
temsilen 30 izolatın teşhisleri, antagonistik ve bitki gelişimini teşvik eden mekanizmaları
araştırılmıştır. Morfolojik, fizyolojik, biyokimyasal ve MALDI-TOF analizler,
elde edilen izolatların 10 cins 24 farklı türe ait olduğunu göstermiştir. Bu
izolatlar arasında 10 izolat Bacillus spp.,
6 izolat Pseudomonas spp., 4 izolat Rhizobium radiobacter, 2 izolat Arthrobacter spp., 2 izolat Achromobacter spanius, 2 izolat Serratia liquefaciens, 1’er izolat Acinetobacter calcoaceticus, Exiguobacterium sp., Microbacterium hydrocarbonoxydans
ve Ochrobactrum anthropi olarak
teşhis edilmiştir. Psp’ye karşı en yüksek
engelleme zonu Pseudomonas gessardii
(4.85) izolatı tarafından, en düşük engelleme zonu ise Bacillus licheniformis (1.35) tarafından oluşturulmuştur. Test
edilen izolatlardan 10 izolat α-amylase pozitif, 7 izolat fosfataz pozitif, 29
izolat siderofor üretmiş, 11 izolat proteaz pozitif bulunmuştur. Tüm izolatlar
IAA ve amonyak üretimi açısından değişken değerlerde pozitif bulunmuştur. En
yüksek siderofor üretimi P. gessardii
(5.83 indeks değeri), en yüksek protease üretimi Exiguobacterium sp. (5.25 indeks değeri), en yüksek IAA üretimi P. gessardii ve O. anthropi izolatları (161.39 µg/ml), en yüksek fosfor çözme
kapasitesi Acinetobacter calcoaceticus
izolatı (2.63 indeks değeri) tarafından gösterilmiştir. Bu çalışma ile ilk kez fasulye
bitkisinde bitki gelişimini teşvik eden ve Psp’ye
antagonistik etkiye sahip endofit bakteriler elde edilmiştir.

Keywords

fasulye, hale yanıklığı, antagonist, bitki gelişimi teşvik eden bakteriler, endofit, biyolojik mücadele, Pseudomonas syringae pv. phaseolicola

Project Number

TAGEM-BS-15/09-03/02-07

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