Tıbbi Öneme Sahip Hatmiler İçin Etkili Bir Çimlendirme Yöntemi: Tohum Kabuğuna Çentik Atılması

Year 2019, Volume: 8 Issue: 3, 843 - 851, 30.09.2019

Abdulhamit Battal , Gül Görmez , Musa Türker

https://doi.org/10.17798/bitlisfen.544221

Abstract

Malvaceae familyasının üyelerinden biri olan Alcea cinsi tıbbi öneme sahip bazı
türleri içermektedir. Alcea kurdica
Alef. (Devegülü Hatmi) tonsilite, gastrik ülsere, zatürreye, boşaltım sistemi
enfeksiyonlarına ve saç kırana karşı kullanılan tıbbi bir bitkidir. Bu
çalışmanın amacı, devegülü hatmi bitkisi için steril şartlar altında etkili bir
çimlendirme yönteminin geliştirilmesi ve bu bitkinin doku kültürü
çalışmalarında kullanılabilir hale getirilmesidir. Bu amaç için, steril bir
ajan olan sodyum hipokloritin üç farklı konsantrasyonu (%5, %7.5 ve %10), 42°C
sıcaklıkta inkübasyonu ve tohum kabuğunun bistüri ile çentiklenmesi
uygulamaları test edilmiştir. %5 ve %7.5 sodyum hipoklorit konsantrasyonlarının
sterilizasyon için yeterli olmadığı belirlenirken, %10 sodyum hipoklorit
konsantrasyonun ise yeterli olduğu gözlenmiştir. Çimlenme indeksi sonuçlarına göre, kabuğu çentiklenen tohumlar
hızlıca çimlenirken, 42°C de inkübasyonun çimlenmeyi etkilemediği gözlenmiştir.
Yedinci günde, kotiledon, kotiledon sapı ve hipokotil dokuları doku kültürü
için kullanılabilir duruma gelmiştir. Sekizinci günde, yapraklar oluşmaya
başlamıştır. Yaprak, yaprak sapı ve sürgün ucu eksplantları ise otuzuncu günde
doku kültüründe kullanılabilecek duruma gelmiştir. Sonuç olarak, bu çalışma ile
tıbbi öneme sahip devegülü hatmi için etkili bir çimlendirme yöntemi
geliştirilmiştir.

Keywords

Çimlenme İndeksi, Hatmi, Alcea

The Efficient Germination Method For Marhmallows With Medicinal Importance: The Nicking on Seed Coat

Abstract

The genus Alcea
one of the member of Malvaceae family includes some species having medicinal
importance. Alcea kurdica Alef.
(Devegülü Marshmallow) is a medicinal plant used against tonsils, gastric
ulcers, pneumonia, excretory system infections and pelade. The aim of this
study was to develope an efficient germination method under sterile conditions
and to make the plant suitable for tissue culture studies. For this purpose,
three different concentrations (5%, 7.5% and 10%) of the sodium hypochlorite
which is a sterile agent, 42°C incubation and damaging on the seed coat with
blade were tested. While 5% and 7.5% of sodium hypochlorite concentrations were
not sufficient for sterilization, 10% of sodium hypochlorite concentration was
observed to be sufficient. According to the results of the germination index,
while the seeds nicked on coat were easily germinated, it was observed that
incubation at 42°C did not affect the germination. On the seventh day,
cotyledon, cotyledonary node and hypocotyl tissues were ready to use for tissue
culture. On the eighth day, leaves began to form. Leaf, petiole and shoot tip
explants were available to use in tissue culture on the thirtieth day. As a
result, an efficient germination method for devegülü marshmallow having
medicinal importance was developed with this study.

Keywords

Germination Index, Marshmallow, Alcea

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