Lactic Acid Bacteria Diversity of Koumiss Samples

Year 2020, Volume: 1 Issue: 1-2, 1 - 6, 15.12.2020

Ruslan Adil Akai Tegin , Zafer Gönülalan , Anarseyit Deydiev

Abstract

In this study lactic acid bacteria (LAB) diverstiy of koumiss samples were inverstigated. A total number of 22 koumiss samples were obtained from the pastures of the Naryn region of Kyrgyzstan Republic. Lactic acid bacteria and yeast counts of samples were determined. The identification of LAB strains from koumiss samples was carried out with the PCR, VITEC 2 Compact, and an automated mass spectrometry (MS) microbial-identification system using matrix assisted laser desorption ionization time-of-flight (MALDI-TOF). Lactobacillus helveticus, Lactobacillus kefiri, Leuconostoc mesenteroides, Lactobacillus paraplantarum, Leuconostoc mesenteroides spp cremoris were determined as lactic acid bacteria species. Bacterium like Leuconostoc sp. which is rarely met in koumiss has been identified on the genetic level using PCR. Information from these results could advance our understanding of koumiss fermentation, and also help improve the quality of koumiss.

Keywords

Koumiss, LAB, PCR, MALDI-TOF-MS, Kyrgyzstan

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