In Vitro Multiple Shoot Induction From Embryonic Axes of Annual Herbaceous Legume Faba Bean (Vicia faba L.)

Year 2022, Volume: 5 Issue: 2, 80 - 86, 01.04.2022

Ferzat Turan

https://doi.org/10.47115/bsagriculture.1063435

Abstract

The faba bean (Vicia faba L.) is cultivated in the Mediterranean and Aegean regions only. It is a rich source of protein and an important source of food and feed for human and animal consumption. Faba beans have a narrow genetic base and their improvement through hybridization is not feasible because of high pollen self-incompatibility. This study was carried out using the embryonic axis of cv. Filiz99 and Eresen87 regenerated on MS medium containing 11 different combinations of BAP and NAA. The results showed 100% shoot regeneration frequency with maximum number of 3.3 and 3.5 shoots per explant on cv. Filiz99 and Eresen87 respectively. Regenerating shoots were rooted on 1 mg L–1 IAA. The in vitro regenerated shoots were continuously cultured for 3 weeks to acclimatize them. This approach could improve broad bean seed germination and subsequently regeneration. The results could also facilitate genetic transformation studies.

Keywords

Acclimatisation, BAP, NAA, Zygotic embryos

References

• Abdelwahd R, Hakam N, Labhilili M and Udupa SM. 2008. Use of an adsorbent and antioxidants to reduce the effects of leached phenolics in in vitro plantlet regeneration of faba bean. African J Biotechnol, 7(8): 997-1002.
• Alan Ö, Geren H. 2006. An investigation on the seed yield and other characteristics of some faba bean (Vicia faba var. major) cultivars grown under Ödemis-Izmir ecological conditions. Ege Univ Fac Agri J, 43(1): 13-20.
• Anwar F, Sharmila P, Pardha SP. 2010. No more recalcitrant: Chickpea regeneration and genetic transformation. African J Biotechnol, 9(6): 782-797.
• Anwar F. 2007. Genetic transformation of chickpea with bacterial cod Agene enhancing drought tolerance. PhD Thesis. University of Delhi, Delhi, India.
• Bahgat S, Shabban OA, El-Shihy O, Lightfoot DA, El- Shemy HA. 2009. Establishment of the regeneration system for Vicia faba L. Molec Biol, 11(1): 47-54.
• Bahgat S. 2008. Establishment of the regeneration system for Vicia faba L. Curr Issu Molec Biol, 11(Suppl 1): 47–54.
• Bekkara F, Jay M, Viricel MR, Rome S. 1998. Distribution of phenolic compounds within seed and seedlings of two Vicia faba cvs differing in their seed tannin content, and study of their seed and root phenolic exudations. Plant Soil, 203(1): 27-36.
• Bond DA. 1987. Recent developments in breeding of field beans (Vicia faba L.). Plant Breed, 99: 1-26.
• Böttinger P, Steinmetz A, Schieder O, Pickardt T. 2001. Agrobacterium-mediated transformation of Vicia faba. Biol Breed, 8: 243-254.
• Chance B, Maehly AC. 1955. Assay of catalase and peroxidases. Methods Enzymol, 2: 764-775.
• Davila MA, Sangronis E, Granito M. 2003. Germinated or fermented legumes: Food or ingredients of functional food. Archivos Latinoamericanos De Nutri, 53: 348-354.
• Desjardins Y, Gosselin A, Yelle S. 1987. Acclimatization of ex vitro strawberry plantlets in CO2-enriched environments and supplementary lighting. J American Soc Horticult Sci,112: 846-851.
• Duke JA. 1981. Handbook of legumes of world economic importance. New York Plenum Press, Newyork, US, pp: 265.
• El-Mergawi R, TaieHanan AA. 2014. Phenolic composition and antioxidant activity of raw seeds, green seeds and sprouts of ten faba bean (Vicia faba) cultivars consumed in Egypt. Int J Pharma Bio Sci, 5(2): 609-609.
• FAO. 2017 http://faostat3.fao.org/home/E (access date: October 12, 2021).
• Giannopolitis CN, Ries SK. 1977. Superoxide dismutases: I. occurrence in higher plants. Plant Physiol, 59: 309-314.
• Goncalves JC, Diego G, Coelho MT, Amancio S. 1998. Effect of rooting conditions on survival and growth during acclimatization of micropropagated chestnut plants (Castanea sativa × C. crenata). Acta Hort, 494: 235-241.
• Griga M, Kubalakova M, Tejklova E. 1987. Somatic embryogenesis in Vicia faba L. Plant Cell Tissue Organ Cult, 9: 167-171.
• Gürel S, Oğuz MÇ, Turan F, Kazan K, Özmen CY, Gürel E, Ergül A. 2019. Utilization of sucrose during cocultivation positively affects Agrobacterium-mediated transformation efficiency in sugar beet (Beta vulgaris L.). Turkish J Agri Forest, 43: 509-517.
• Jiang Y, Huang B. 2001. Drought and heat stress injury to two cool-season turfgrasses in relation to antioxidant metabolism and lipid peroxidation. Crop Sci, 41: 436-442.
• Khalafalla M, Hattori H. 2000. Ethylene inhibitors enhance in vitro root formation on faba bean shoots regenerated on medium containing thidiazuron. Plant Growth Regul, 32: 59-63.
• Klenotičova H, Iva S, Sbavoba L, Miroslav M. 2013. Resolving browning during the establishment of explant cultures in Viciafaba L. for genetic transformation. Acta Univ Agri Silvia Cult Mendelianae Brun, LXI(5): 1279-1288.
• Kuchuk N. 2001. Biotechnology In: Carbohydrates in grain legume seeds, C.L. Hedly, ed. CABI Publishing, Wallingford, UK, pp: 207.
• Kuplemez H, Yildirim MU. 2020. Effects of cytokinin and auxin on plant development and vascular tissues in lens culinaris. Commagene J Biol, 4(1): 16-21.
• Messina MJ. 1999. Legumes and soybeans: overview of their nutritional profiles and health effects. American J Clin Nutri, 70: 439-450.
• Murashige T, Skoog F. 1962. A revised medium for rapid growth and bio assays with tobacco tissue cultures. Physiol Plant, 15: 473-497.
• Paul SR, Chodhury AK. 1991. Effect of seed priming with potassium salts on growth and yield of wheat under rain fed condition. Annual Agri Res, 12: 415-418.
• Pekşen E, Artik C. 2006. Determination of plant characteristics and seed yield of some local faba bean (Vicia faba L.) populations. Tarim Bil Derg, 12(2): 166-174.
• Rahate KA, Madhumita M, Prabhakar PK. 2020. Nutritional composition, anti-nutritional factors, pre-treatments-cum-processing impact and food formulation potential of faba bean (Vicia faba L.): A comprehensive review. LWT - Food Sci Technol, 138: 110796.
• Rowlands DG. 1964. Fertility studies in the broad bean (Vicia faba L). Heredity, 19: 271-277.
• Schulze S, Grunewald J, Schmidt H. 1985. Zur in vitro-Regeneration von Vicia faba L. Z Pflanzenzucht, 94: 244-250.
• Selva E, Stouffes B, Briquet M. 1989. In vitro propagation of Vicia faba L. by micro-cutting and multiple shoot induction. Plant Cell Tissue Organ Cult, 18: 167-179.
• Siddiqui MH, Al-Whaibi MH, Sakran AM, Basalah MO, Ali HM. 2012. Effect of calcium and potassium on antioxidant system of Vicia faba L. under cadmium stress. Int J Molec Sci, 13: 6604-6619.
• Stoddard FL, Bond DA. 2015. The pollination requirements of the faba bean. Bee World, 68(3): 144-152.
• Taha RM, Francis D. 1990. The relationship between polyploidy and organogenetic potential in embryo and root-derived tissue cultures of Vicia faba L. Plant Cell Tissue Organ Cult, 22: 229-236.
• Tegeder M, Gebhardt D, Schieder O, Pickardt T. 1995. Thidiazuron-induced plant regeneration from protoplasts of Vicia faba cv. Mythos Plant Cell Rep, 15: 164-169.
• Urbano G, Aranda P, Go mez-Villalba E, Freijnagel S, Porres JM, Frìas J. 2003. Nutritional evaluation of pea (Pisum sativum L.) protein diets alter mild hydrothermal treatment and with and without added phytase. J Agri Food Chem, 51: 2415-2420.
• Vidal-Valverde C, Frias J, Herna´ndez A, Martìn-Alvarez PJ, Sierra I, Rodrguez C. 2003. Assessment of nutritional compounds and antinutritional factors in pea (Pisum sativum) seeds. J Sci Food Agri, 83: 298-306.
• Yildirim MU. 2019. In vitro plant regeneration and antibacterial activities of gentiana lutea-an endangered medicinal plant species. Fresenius Environ Bull, 28(7): 5070-5074.
• Zaats AJ, Wijffelmacn A, Spainkh P, Van Brussela AN, Okkerr JH, Lugtenberg BJJ. 1987 Induction of the nodA promoter of Rhizobium leguminosarum sym plasmid pRLl JI by plant flavanones and flavones. J Bacteriol, 169: 198-204.