Effect of photooxidative stress and mannitol on synthesis of OmpC-OmpF proteins of Escherichia coli in lake water

Year 2019, Volume: 6 Issue: 2, 251 - 260, 26.12.2019

Cihan Darcan , Öznur Aktop

https://doi.org/10.35193/bseufbd.610434

Abstract

This study investigated the effects of photooxidative
stress and mannitol on the synthesis of OmpC-OmpF porins of Escherichia coli in lake water. The
synthesis of OmpF decreased independently of photooxidative stress, whereas the
synthesis of OmpC decreased as a consequence of photooxidative stress in lake
water. Thus, OmpC synthesis in E. coli was
directly affected by photooxidative stress. Mutations in the envZ and pta genes had no effect on the control of OmpC and OmpF synthesis
in E. coli under photooxidative
stress in lake water. Mannitol is an antioxidant substance that provides
protection from photooxidative stress. In this study, was found that ompC expression has a regulation
mechanism during photooxidative stress. Mannitol was also found to have a
relationship with EnvZ in the control of porin synthesis.

Keywords

AcP, EnvZ, Lake water, Mannitol, Photooxidative

Göl suyunda Escherichia coli'nin OmpC-OmpF proteinlerinin sentezi üzerine mannitol ve fotooksidatif stresin etkisi

Abstract

Bu çalışma göl suyunda Escherichia coli'nin OmpC-OmpF porinlerinin sentezi üzerine mannitol ve fotooksidatif stresin etkisini araştırmıştır. Göl suyunda OmpF sentezi fotooksidatif stresten bağımsız olarak azalmışken, OmpC sentezi fotooksidatif stresin bir sonucu olarak azalmıştır. Bu yüzden, E. coli'de OmpC sentezi  direkt olarak fotooksidatif stresten etkilenmektedir. envZ ve pta genlerindeki mutasyonların göl suyunda fotooksidatif stres altında E. coli'de OmpC ve OmpF sentezinin kontrolü üzerine bir etkisi yoktur. Mannitol fotooksidatif stresden koruyan bir antioksidant moleküldür. Bu çalışmada ompC ekspresyonu fotooksidatif stres altında bir düzenleme mekanizmasına sahiptir. Aynı zamanda mannitol'ün porin sentezinin kontrolünde EnvZ ile bir ilişkisi olduğu bulunmuştur. 

Keywords

AcP, EnvZ, Lake water, Mannitol, Fotooksidatif

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