Insecticidal Activity of Prodigiosin Pigment on Tenebrio molitor (Coleoptera: Tenebrionidae)

Year 2022, Volume: 9 Issue: 2, 1035 - 1040, 31.12.2022

Ardahan Eski , Tayyibe Özdemir

https://doi.org/10.35193/bseufbd.1172976

Cited By: 2

Abstract

Microorganisms and their metabolites have the potential to provide a sustainable alternative to chemical insecticides. Serratia marcescens is an entomopathogenic bacterium that produces hydrolytic enzymes and toxins. It also produces a red pigment called prodigiosin, which has a variety of biological properties. In this study, the prodigiosin pigment was extracted from S. marcescens Se9 isolate with acidified ethanol and concentrated using a rotary evaporator. The insecticidal potential on larval and adult stages of Tenebrio molitor was then investigated. It was found that the mortality rate of larvae exposed to the lowest concentration (125 ppm) of crude pigment was 5%, while the mortality rate of larvae exposed to the highest concentration (2000 ppm) was 68%. The LC50 value of the crude pigment for the larval stage was determined to be 924 ppm. On the other hand, even at the highest pigment concentration (2000 ppm), only 30% mortality was observed in adults. The LC50 value of the crude pigment for the adult stage was determined to be 4570 ppm. It was determined that the pigment had a toxic effect on the pest, but the larval stage was more sensitive than the adult stage. The study showed that prodigiosin pigment appears to be a promising biocontrol agent for use against T. molitor. 

Keywords

Serratia marcescens, Bacterial pigment, Biocontrol

Supporting Institution

TÜBİTAK

Project Number

1919B012102186

Thanks

This study was financially supported by the research project support programme for undergraduate students (2209-A, Project no: 1919B012102186), TUBITAK.

Prodigiosin Pigmentinin Tenebrio molitor (Coleoptera:Tenebrionidae) Üzerindeki İnsektisidal Aktivitesi

Abstract

Mikroorganizmalar ve metabolitleri, kimyasal insektisitlere sürdürülebilir bir alternatif sağlama potansiyeline sahiptir. Serratia marcescens hidrolitik enzimler ve toksinler üreten entomopatojenik bir bakteridir. Ayrıca, çeşitli biyolojik özelliklere sahip olan, prodigiosin adı verilen kırmızı bir pigment üretir. Bu çalışmada, prodigiosin pigmenti, asitleştirilmiş etanol ile S. marcescens Se9 izolatından ekstrakte edildi ve bir döner buharlaştırıcı kullanılarak konsantre edildi. Daha sonra Tenebrio molitor'un larva ve ergin evreleri üzerindeki insektisidal potansiyeli araştırıldı. Ham pigmentin en düşük konsantrasyonuna (125 ppm) maruz kalan larvaların ölüm oranının %5, en yüksek konsantrasyona (2000 ppm) maruz kalan larvaların ölüm oranının ise %68 olduğu tespit edildi. Larva evresi için ham pigmentin LC50 değeri 924 ppm olarak belirlendi. Öte yandan, en yüksek pigment konsantrasyonunda (2000 ppm) bile erginlerde sadece %30 ölüm gözlendi. Ham pigmentin ergin evresi için LC50 değeri 457 ppm olarak belirlendi. Pigmentin zararlı üzerinde toksik etkisinin olduğu ancak larva döneminin ergin döneme göre daha duyarlı olduğu belirlendi. Çalışma, prodigiosin pigmentinin, T. molitor'a karşı kullanım için umut verici bir biyokontrol ajanı olabileceğini gösterdi.

Keywords

Serratia Marcescens, Bakteriyal Pigment, Prodigiosin, Biyokontrol, Serratia marcescens, Bacterial pigment, Biocontrol

Project Number

1919B012102186

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