İn Vitro, İn Vivo Ve Klinik Çalışmalarla Kanser Tedavisinde Kalsiyum Elektroporasyonun Değerlendirilmesi

Year 2022, Volume: 5 Issue: 3, 585 - 590, 01.09.2022

Güney Gürsoy , Meric Esmekaya , Zehra Çiçek

https://doi.org/10.19127/bshealthscience.1092589

Abstract

Tıp ve biyoteknolojide birçok uygulamaya sahip olan elektroporasyon (EP), hücre membranını harici, kısa ve yüksek voltajlı elektrik pulsları ile geçirgen hale getirmek için kullanılan bir yöntemdir. EP sonrasında hücre membranı boyunca artan molekül akışı gözlenir. Elektrokemoterapi (EKT), kemoterapi ile EP tekniğini birleştirerek tümörlerin palyatif tedavisi için kullanılmaktadır. Birçok anti-kanser uygulaması, kalsiyum (Ca+2) sinyallerini etkileyerek kanser tedavisinde hedef olarak Ca+2 yolaklarını desteklemektedir. EKT uygulamasının yanı sıra Ca+2 elektroporasyonun (CaEP) kullanılması ATP miktarının azalmasına bağlı olarak tümör hücrelerinin ölümüne neden olur. Bu derlemede, CaEP’nin mevcut in vitro, in vivo ve klinik çalışmaların deney sonuçlarının gözden geçirilmesi ve yeni bir anti-kanser tedavi yöntemi olarak değerlendirilmesi amaçlanmıştır.

Keywords

Elektroporasyon, Kalsiyum elektroporasyon (CaEP), Kanser

Evaluation of Calcium Electroporation in Cancer Treatment with In Vitro, In Vivo and Clinical Studies

Abstract

Electroporation (EP), which has many applications in medicine and biotechnology, is a method used to make the cell membrane permeable with external, short and high voltage electrical pulses. After EP, an increased flow of molecules across the cell membrane is observed. Electrochemotherapy (ECT) is used for palliative treatment of tumors by combining chemotherapy and EP technique. Many anti-cancer applications support Ca2+ pathways as a target in cancer treatment by affecting calcium (Ca2+) signals. The use of Ca2+ electroporation (CaEP) instead of ECT application causes the death of tumor cells due to the decrease in the amount of ATP. In this review, it is aimed to review the experimental results of existing in vitro, in vivo and clinical studies of CaEP and to evaluate it as a new anti-cancer treatment method.

Keywords

Electroporation, Calcium electroporation (CaEP), Cancer

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