Exogenous Polyamine Treatment Prevents UV-A Induced Cellular Damage and Photoinhibition of Photosystem II in Lichen Physcia semipinnata (J. F. Gmel.) Moberg

Year 2018, , 399 - 404, 28.12.2018

Dilek Ünal , İnci Tüney Kızılkaya

https://doi.org/10.18466/cbayarfbe.410546

Abstract

In this study the protection ability of polyamines on
Photosystem II (PSII), the proline content of the cells and necrosis formation
induced by Ultraviolet-A irradiation were studied. Although 24 h Ultraviolet-A
exposure decreased the photosynthetic quantum yield (Fv/Fm) ratio in Physcia semipinnata (J. F. Gmel.) Moberg,
thalli that treated with 1 mM polyamine, especially spermidine and spermine,
were slightly influenced by the UV-A exposure. It was also found that spd and
spm treated samples had lower proline content than putrescine (put)-treated samples.
Moreover, It was found that the samples that were treated with spd and spm had
lower percentage of DNA damage than put-treated samples and non-treated groups.
In this study, Ultraviolet-A induced DNA damage detected by acridine
orange/ethidium bromide staining assay. The DNA damage in thalli of P. semipinnata
was detected in situ in phycobiont layer after 24 h of Ultraviolet-A exposure,
in contrast, polyamine-treated samples had a lower rate of necrotic cells.

Keywords

polyamine, photosystem II, proline, UV-A, lichen

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