Visualization of Human Kidney Embryonic Cells by Acoustic Microscopy

Year 2018, , 455 - 460, 28.12.2018

Bükem Bilen , Nural Pastaci Ozsobaci , Fatma Ates Alkan , Mehmet Burcin Unku

https://doi.org/10.18466/cbayarfbe.454158

Abstract

The aim of our study is to
evaluate the capability of acoustic microscopy with a high frequency transducer
for visualization of cells. Human kidney embryonic (HEK293) cells were observed
by scanning acoustic microscope (SAM). HEK293 cells cultured on cover glasses
were kept in cell culture plates. A transducer with 320 MHz center frequency
and a spot size of 4.0 µm was incorporated into SAM. Two-dimensional acoustic
images were obtained successfully to identify the morphology and the acoustic
properties of HEK293 cells and the acoustic impedance of HEK293 cells was
measured as 1.65 ±
0.02 MRayl. The same cells on cover glasses were observed also with inverted
microscope. The results in this study indicate that scanning acoustic
microscopy can be used for the determination of elastic properties of cells,
therefore for cellular mapping and molecular imaging, since it can create very
sharp images of HEK293 cells with a high frequency transducer.

Keywords

Scanning acoustic microscopy, kidney cell, acoustic impedance

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