Effects of Zinc oxide Nanoparticles (ZnO NPs) on Hemocyte Types of Galleria mellonella

Year 2022, , 207 - 212, 30.06.2021

Ata Eskin

https://doi.org/10.18466/cbayarfbe.989240

Abstract

In this study, 70 nm-sized and nanorod-shaped Zinc oxide nanoparticle (ZnO NPs) concentrations (0.5, 1, 2.5, 5, and 10 µg/10 µl) was force-fed to fourth instar (110 ± 20 mg) Galleria mellonella (Lepidoptera: Pyralidae) larvae. The effects of ZnO NPs on plasmatocyte, granulocyte, spherulocyte, prohemocyte, oenocytoid, and coagulocyte numbers in hemolymph of G. mellonella larvae was determined. Results showed that treating G. mellonella with 10 μg/10 µl ZnO NPs significantly decreased spherulocytes numbers, whereas numbers of plasmatocyte, granulocyte, prohemocyte, oenocytoid, and coagulocyte numbers did not differ significantly when compared to the control group after 24 h force feeding treatment. There was a statistically significant difference between the experimental groups in the prohemocyte numbers of larvae that exposed to 1 and 5 μg/10 µl ZnO NPs.

Keywords

Galleria mellonella, Hemocyte Type, Nanoparticle, Zinc oxide

References

• 1. 1. Miller, JC, Ruben S, Jose Miguel Represas-Cardenas, and Griffith K. The handbook of nanotechnology: Business, policy, and intellectual property law. John Wiley & Sons, 2004.
• 2. 2. Tuncsoy, B, & Mese, Y 2021. Influence of titanium dioxide nanoparticles on bioaccumulation, antioxidant defense and immune system of Galleria mellonella L. Environmental Science and Pollution Research, 1-9.
• 3. 3. Mir, AH, Qamar, A, Qadir, I, Naqvi, AH, & Begum, R 2020. Accumulation and trafficking of zinc oxide nanoparticles in an invertebrate model, Bombyx mori, with insights on their effects on immuno-competent cells. Scientific reports, 10(1), 1-14.
• 4. 4. Moya-Andérico, L, Vukomanovic, M, del Mar Cendra, M, Segura-Feliu, M, Gil, V., José, A & Torrents, E 2021. Utility of Galleria mellonella larvae for evaluating nanoparticle toxicology. Chemosphere, 266, 129235.
• 5. 5. Eskin, A, Öztürk, Ş, & Körükçü, M 2019. Determination of the acute toxic effects of zinc oxide nanoparticles (ZnO NPs) in total hemocytes counts of Galleria mellonella (Lepidoptera: Pyralidae) with two different methods. Ecotoxicology, 28(7), 801-808.
• 6. 6. Eskin, A, Altinkaynak, C, Merve, T & Özdemir, N 2020. Fluorescent copper phosphate nanoflowers: a novel toxicity investigation study based on Tenebrio molitor Linnaeus, 1758 (Coleoptera: Tenebrionidae) larvae. Bitlis Eren Üniversitesi Fen Bilimleri Dergisi, 9(3), 975-984.
• 7. 7. Eskin, A, & Bozdoğan, H 2021. Effects of the copper oxide nanoparticles (CuO NPs) on Galleria mellonella hemocytes. Drug and Chemical Toxicology, 1-11.
• 8. 8. Eskin, A, Ekremoglu, M, Altinkaynak, C, & Özdemir, N 2021. Effects of organic-inorganic hybrid nanoflowers’ framework on hemocytes and enzymatic responses of the model organism, Galleria mellonella (Lepidoptera: Pyralidae). International Journal of Tropical Insect Science, 1-12.
• 9. 9. Matha, V, & Áček, Z (1984). Changes in haemocyte counts in Galleria mellonella (L.)(Lepidoptera: Galleriidae) larvae infected with Steinernema sp.(Nematoda: Steinernematidae). Nematologica, 30(1), 86-89.
• 10. 10. Champion, OL, Wagley, S, & Titball, RW 2016. Galleria mellonella as a model host for microbiological and toxin research. Virulence, 7(7), 840-845.
• 11. 11. Ramarao, N, Nielsen-Leroux, C, Lereclus, D 2012. The insect Galleria mellonella as a powerful infection model to investigate bacterial pathogenesis, Journal of Visualized Experiments, 70: 4392.
• 12. 12. Shaban, HE, Helal, IB, Shamseldean, MM, & Seif, AI 201. Defensive reactions of Periplaneta americana (Blattodea: Blattidae) adults against Steinernema Sp. 2d (Rhabditida). The Egyptian Society of Experimental Biology, 6(2): 263-271.
• 13. Blanco, LAA, Crispim, JS, Fernandes, KM, de Oliveira, LL, Pereira, MF, Bazzolli, DMS, & Martins, GF 2017. Differential cellular immune response of Galleria mellonella to Actinobacillus pleuropneumoniae. Cell and Tissue Research, 370(1), 153-168.
• 14. IBM-SPSS Statistics for Windows. 2011. Version 20.0. Elsevier, London, UK. IBM Corp. Released. Armonk, NY: IBM Corp.
• 15. Ribeiro, C, Brehélin, M, 2006. Insect hemocytes: What type of cell is that? Journal of Insect Physiology. 52, 417–429.
• 16. Mizerska‐Dudka, M, & Andrejko, M 2014. Galleria mellonella hemocytes destruction after infection with Pseudomonas aeruginosa. Journal of Basic Microbiology, 54(3), 232-246.
• 17. Gupta, A 1979. Insect Hemocytes: Development, Forms, Functions and Techniques. Cambridge: Cambridge University Press. doi:10.1017/CBO9780511759987.
• 18. Arnold, JW 1974. The hemocytes of insects, pp. 201-54. In M. Rockstein (ed.). The Physiology of Insecta, Vol. 5, 2nd ed. Academic Press, New York.
• 19. Mir, AH, Qamar, A, Qadir, I, Naqvi, AH, & Begum, R 2020. Accumulation and trafficking of zinc oxide nanoparticles in an invertebrate model, Bombyx mori, with insights on their effects on immuno-competent cells. Scientific Reports, 10(1), 1-14.
• 20. Teleb, SS 2011. Effect of Nomolt on differential and total haemocytes in the desert locust Schistocerca gregaria Forskal (Orthoptera: Acrididae). Journal of American Science, 7(11), 479-484.
• 21. Tojo S, Naganuma F, Arakawa1 K, Yokoo1 S 2000. Involvement of both granular cells and plasmatocytes in phagocytic reactions in the greater wax moth, Galleria mellonella. Journal of Insect Physiology, 46:1129–1135.
• 22. Pereira, TC, De Barros, PP, Fugisaki, LRDO, Rossoni, RD, Ribeiro, FDC, De Menezes, RT, ... & Scorzoni, L 2018. Recent advances in the use of Galleria mellonella model to study immune responses against human pathogens. Journal of Fungi, 4(4), 128.
• 23. Gelbič, I, Stráčková, J, & Berger, J (2006). Influence of metyrapone on the morphology of hemocytes of the Egyptian cotton leafworm Spodoptera littoralis (Boisd.). Zoological Studies, 45(3), 371-377.
• 24. Bohn, H 1986. Hemolymph clotting in insects. Immunity in Invertebrates, 188-207.
• 25. Brehélin, M and Zachary, D, 1986. Insect haemocytes: a new classification to rule out the controversy. In Immunity in Invertebrates (pp. 36-48). Springer, Berlin, Heidelberg.
• 26. Essawy, M, A Maleville, and M. Brehélin. 1984. Evolution of haemogram during the larval development (last instar) of Eliothis armigera. Invertebr Immunol Conf 17-29 Sept 1984, Montpellier, France." Dev Comp Immunol.
• 27. Pathak, JPN 1986. Haemogram and its endocrine control in insects. In Immunity in Invertebrates, pp. 49-59. Springer, Berlin, Heidelberg, 1986.