Evaluating of Salt Stress Tolerance in Selected Wheat Mutant Progenies with Contributing Expression Analysis of TaWRKY Genes and Antioxidant Defence Parameters

Year 2018, Volume: 14 Issue: 3, 315 - 320, 30.09.2018

Ayşe Şen , Fatma Sarsu

https://doi.org/10.18466/cbayarfbe.430620

Cited By: 2

Abstract

In current work, the seeds from two candidate
fifth-generation moderately salt tolerant wheat mutant progenies (Triticum aestivum L. cv. Adana 99)
developed with using sodium azide (as a mutagenic agent) and from their parent
(commercial cultivar) were sown in the growth chamber to evaluate their
performance under control and 150 mM NaCl treatment conditions. Compared with
commercial cultivar, mutants demonstrated much higher activities of superoxide
dismutase, catalase and guaiacol peroxidase, and chlorophyll contents, and much
lower contents of lipid peroxidation and values of electrolyte leakage under
control condition. On the other hand, contents of lipid peroxidation and value
of electrolyte leakage increased, but chlorophyll content decreased more severely in
commercial cultivar than mutants under salt stress condition. In addition to increasing in
expression levels of TaWRKY5, TaWRKY10 and TaWRKY44 genes in mutants positively regulated in salt stress
responses by either direct or indirect activation of the cellular antioxidant
enzyme responses or activation of stress-related gene expression. These
candidate salt tolerant wheat mutant progenies will be used to increase the
salt tolerance in the wheat-breeding programmers.

Keywords

Antioxidant enzymes, salt stress tolerance, sodium azide mutagenesis, wheat, qPCR, WRKY transcription factors

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