STUDY OF THE CHANGE IN ANTIOXIDATIVE ENZYME ACTIVITIES, METAL ACCUMULATION, SOME PHYSIOLOGICAL AND GROWTH PARAMETERS OF SHOOT AND ROOT IN ROCA (ERUCA SATIVA) PLANT

Year 2010, Volume: 6 Issue: 2, 105 - 120, 01.06.2010

Seda Çınar Becerik Tülini Aydemir

Abstract

In this study, we aimed to compare some antioxidative responses, which is associated with the growth reduction, of shoots and roots of Eruca sativa plant in the presence of different concentration of Mn+2 (10 - 5000 µM) or Fe+2 (50 – 5000 µM). The excess of Mn+2 or Fe+2 caused a significant inhibition on the plant growth. Significant increases in MDA, SOD, GPX, proline, electrolytic leakage and H2O2 levels were observed in both shoots and roots stressed under Mn+2 and Fe+2. Mn+2 treatment caused a greater decrease in growth than Fe+2 treatment in both tissues. The contents of total chlorophyll and carotenoid in the leaves of the plant were also reduced by the increasing the Mn+2 or Fe+2 concentrations. Cu uptake was increased in the presence of excess Mn+2 in both tissues, although Fe+2 and Zn+2 contents were significantly reduced. Consequently, it can be concluded, that the high Mn+2 or Fe+2 contents in growth media can be the responsible for the growth inhibition by enhancing the oxidative stress. 

Keywords

Roca (Eruca sativa) plant, Mn+2 and Fe+2 stresses, metal uptake, lipid peroxidation, H2O2 content, proline content

ROKA (ERUCA SATİVA) BİTKİSİNİN KÖK VE GÖVDESİNDEKİ ANTİOKSİDAN ENZİM AKTİVİTELERİ, METAL BİRİKİMİ, BAZI FİZYOLOJİK VE BÜYÜME PARAMETRELERİNDEKİ DEĞİŞİMLERİN ARAŞTIRILMASI

Abstract

Bu çalışmada, farklı Mn+2 (10 - 5000 µM) veya Fe+2 (50 – 5000 µM) konsantrasyonlarının varlığında Eruca sativa bitkisindeki büyüme geriliği ile ilişkili olarak köklerde ve gövdede antioksidatif cevabın karşılaştırılmasını amaçladık. Aşırı Mn+2 veya Fe+2 bitkilerin büyümesini anlamlı olarak inhibe etti. Her iki dokuda da, Mn+2 ve Fe+2 stresinde MDA, prolin ve H2O2 seviyelerinde, elektrolitik iletkenlik, SOD ve GPX aktivitelerinde anlamlı artış izlendi. Eruca sativa bitkisinde, dışarıdan artan konsantrasyonlarda uygulanan Mn+2 ve Fe+2, bitkinin köklerinde ve gövdesinde uygulanan metalin aşırı birikimine sebep oldu. Aşırı Mn+2 köklerde ve gövdede Fe+2 ve Zn+2 alınımını anlamlı olarak azaltırken Cu içeriğini arttırdı. Sonuç olarak, yüksek Mn+2 veya Fe+2 içeriği oksidatif stresi arttırarak büyümenin baskılanmasından sorumlu tutulabilir.

Keywords

Roka (Eruca sativa) bitkisi, Mn+2 ve Fe+2 stresi, metal alımı, lipid peroksidasyonu, H2O2 içeriği, proline içeriği

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• Geliş Tarihi:24/08/2010
• Kabul Tarihi:28/09/2010