Investigation of Effective Immobilization Method for Ethanol Producing E. coli Strain

Year 2019, Volume: 15 Issue: 2, 217 - 220, 30.06.2019

Meltem Yesilcimen Akbas , Taner Sar

https://doi.org/10.18466/cbayarfbe.528451

Cited By: 2

Abstract

There is a growing interest to find alternative
cheap carbon sources for bioethanol production. Whey powder is a by-product of
cheese industry with high lactose content. Immobilization of bacteria is an
effective method for enhancing production of their metabolites including bioethanol.
In this work, a new and effective immobilization method for bioethanol
production from whey powder as a cheap/renewable carbon source was investigated.
For this, different immobilization methods were evaluated to avoid disruption
of bead structures during ethanol fermentation. The best results were obtained
when sodium alginate was sterilized under the UV lamp instead of heating for
immobilization, and the fermentation media was supplemented with CaCl₂
as a stability factor. In addition, the beads were successfully reused in at
least three 48 h batch fermentations for ethanol production

Keywords

E.coli, ethanol, immobilization, repeated batch fermentation, whey powder, lactose

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