In Vitro Bovine Embryo Production: The Role of FCS and Cysteamine on Cleavage Rate

Year 2020, Volume: 7 Issue: 2, 136 - 139, 30.06.2020

Asiye İzem Sandal

https://doi.org/10.34087/cbusbed.624912

Abstract

The aim of this study was to investigate the effects of Cysteamine (C)
and Fetal Calf Serum (FCS) on cleavage rates in bovine embryo production. The oocytes were obtained from the slaughtered ovaries by slicing
method. They were selected and divided into two groups and maturated in TCM and
TCM+Cysteamine for 24 hour at 38.8ºC in atmosphere of 5% CO₂ in
humidified air. The matured oocytes were
transferred to IVF-TALP and allowed to fertilize with frozen bull semen to be 400-600x10^3/well which
were washed with Sperm-TALP in an incubator containing 5% CO₂ for
18-22 hours at 38.8ºC. At the end of this period, the embryos of each group
which were denuded by vortexing transferred to Synthetic Oviduct Fluid (SOF)
and Charles and Rosenkrans (CR1) media to be developed. These culture media
were divided into 4 subgroups according to whether they contain Cysteamine (C)
and Fetal Calf Serum (FCS). For this reason Group A1: (SOF C+F+), Group
A2: (SOF C+F-), Group A3: (SOF C-F+), Group A4: (SOF C-F-), Group B1: (CR1
C+F+), Group B2: (CR1 C+F-), Group B3: (CR1 C-F+), Group B4: (CR1 C-F-) were
formed. Embryos were cultured in an incubator containing 5% CO₂, 5%
N₂ and 90% humidity at 38.8ºC for 48 hours. At the end of this
period, the clevage rate of the embryos were recorded and there was no
statistical difference (P<0.05) between all groups.

Keywords

bovine, cleavage, cysteamine, embryo, fetalcalfserum

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