Development of Recombinant Inbred Line Population in Bread Wheat Using Speed Breeding Technology

Year 2022, Volume: 1 Issue: 2, 36 - 57, 01.12.2022

Bedrettin Demir Yaşar Karaduman , Nevzat Aydın

Abstract

Rye translocation is a significant genetic resource widely used in bread wheat breeding, but it neg-atively affects the bread-making quality. A recombinant inbred line population (RILs) containing different glutenin subunit (GS) combinations of High and Low Molecular Weight (HMW and LMW) and also carries rye translocation or not was developed in the study. We used speed breeding tech-nology and the single seed descent method to develop recombinant lines (RILs). The plants were grown in the plant growth room using Light-Emitting Diode (LED) lamps with a broad spectrum in 22 hours of light and 2 hours of darkness. A generation period ranged between 50-70 days. We obtained two hundred eighty seven inbred lines in F5:6 generation of the RILs population containing 16 different HMW-GS and LMW-GS combinations, genetically anticipated. Ninety-nine of the RILs carried 1BL.1RS rye translocation. As a result of the Gluten Swelling Index (GSI) analysis performed to determine the protein quality, it was determined that the lines carrying the Glu-B3b allele had a GSI value of 3.089, while the lines carrying the rye translocation (Glu-B3j allele) had a GSI value of 3.013. The protein content was found to be 21.41% in the lines carrying the Glu-B3b allele and 20.33% in the lines carrying the Glu-B3j allele. The RILs could be used to investigate the effect of HMW-GS and LMW-GS and rye translocation on the quality properties of bread wheat. The results showed that speed breeding technology is helpful to develop research material in bread wheat in a short period.

Keywords

Speed Breeding, Rye Translocation

Project Number

Bu makale, Karamanoğlu Mehmetbey Üniversitesi, Bilimsel Araştırma Projeleri Koordinatörlüğü tarafından desteklenen 01-D-20 nolu proje kapsa-mında elde edilen verilerden üretilmiştir.

Hızlı Islah Teknolojisi KullanılarakEkmeklik Buğdayda Rekombinant Kendilenmiş Hat Popülasyonunun Geliştirilmesi

Abstract

Çavdar translokasyonu buğday ıslahında yaygın olarak kullanılan önemli bir genetik kaynak olmakla birlikte buğdayın ekmeklik kalitesini olumsuz yönde etkilemektedir. Bu araştırmada farklı Yüksek ve Düşük Molekül ağırlıklı glütenin alt ünite (YMA-GA, DMA-GA) kombinasyonlarını taşıyan aynı zamanda çavdar translo-kasyonu taşıyıp taşımamasına göre genetik bir açılım gözlenen rekombinant kendilenmiş hat popülasyonu geliştirilmiştir. Rekombinant kendilenmiş hat popülasyonunun geliştirilmesinde hızlı ıslah teknolojisi ve tek tohum soy yöntemi kullanılmıştır. Bir generasyon süresi 50-70 gün arasında değişmiştir. F5:6 genera-syonunda 287 adet kendilenmiş hattın elde edildiği araştırmada genetik olarak beklenen 16 farklı YMA-GA ve DMA-GA kombinasyonu gözlenmiştir. Geliştirilen hatların 99 adedinin 1BL.1RS çavdar translokasyonu taşıdığı belirlenmiştir. Protein kalitesinin belirlenmesi için yapılan Gluten Şişme İndeksi (GSI) analizi sonu-cunda Glu-B3b alleli taşıyan hatların 3.089 değerine sahip iken, çavdar translokasyonu taşıyan (Glu-B3jalleli) hatların 3.013 değerine sahip olduğu saptanmıştır. Protein oranının ise Glu-B3b alleli taşıyan hatlarda %21.41, Glu-B3j alleli taşıyan hatlarda %20.33 olduğu saptanmıştır. Elde edilen rekombinant kendilenmiş hat popülasyonu çavdar translokasyonu ile YMA-GA ve DMA-GA ünitelerinin ekmeklik buğdayın kalite özel-liklerine etkisinin araştırılabileceği materyal olarak kullanılabilir. Araştırma sonuçları hızlı ıslah teknolojisinin buğdayda kısa sürede araştırma materyali geliştirmek için etkin olarak kullanılabileceğini göstermiştir.

Keywords

Hızlı Islah, Çavdar Translokasyonu, buğday ıslahı

Ethical Statement

Çalışma için etik kurul izni gerekmemektedir.

Project Number

Bu makale, Karamanoğlu Mehmetbey Üniversitesi, Bilimsel Araştırma Projeleri Koordinatörlüğü tarafından desteklenen 01-D-20 nolu proje kapsa-mında elde edilen verilerden üretilmiştir.

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