THE INVESTIGATION OF IN VITRO CALLUS FORMATION, REGENERATION AND MICROPROPAGATION OF DEVEGULU HOLLYHOCK, A MEDICINAL PLANT

Year 2019, Volume: 6 Issue: 2, 111 - 123, 27.12.2019

Gül Görmez , Abdulhamit Battal , Musa Türker

Abstract

Devegülü hollyhock is used by
public for the treatment of certain diseases. This study investigated the
appropriate protocols for in vitro
callus formation, regeneration and micropropagation of various explants of
14-day and 28-day old Devegülü seedlings germinated from the seeds in a sterile
environment. Different concentrations of 2,4-
dichlorophenoxy acetic acid (2,4-D) were used as plant
growth regulator and Murashige and Skoog basal medium was used as basic medium.
Regenerated plantlets were firstly transferred to rooting medium to promote
root formation, after that they were planted into soil. Both 14-day and 28-day
explants produced a higher rate of callus in MS medium including 2,4-D auxin
hormone. The regenerated plantlets were obtained from the shoot tip and node explants
of 28 days old seedlings after incubation in MS medium for 6 weeks. Direct
regeneration from the node and shoot tip was observed in this hormone free
medium. Micropropagation rates were 44.4 % and 12.5 % for node and shoot tip
explants, respectively. With this study, callus formation, regeneration and
micropropagation of the shoot tip and nodes was successfully carried for the
first time.

Keywords

Devegülü, In Vitro Regeneration, Callus, Hollyhock

Supporting Institution

VAN YÜZÜNCÜ YIL UNIVERSITY

Project Number

FDK-2017-6160

Thanks

We would like to thank Dr. Abdullah Dalar and Dr. Muzaffer Mükemre for their help in collecting and identifying the seeds of Devegülü. This study was supported by Van Yüzüncü Yıl University Scientific Research Projects Department (FDK-2017-6160).

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