Investigation of the effects of sodium phenylpyruvate on pulmonary adenocarcinoma (A549) and mammary adenocarcinoma (MDA-MB-231) on cell lines

Year 2022, Volume: 9 Issue: 1, 20 - 34, 30.06.2022

Abdullah Doğan , Ali Nazmi Can Doğan

https://doi.org/10.48138/cjo.979147

Abstract

Kanser tedavisinde son yıllarda başarı oranını artıran ilaç ve yöntemler geliştirilmiştir. Ancak toksisitesi düşük ve yüksek derecede etkili bir ilaç üretilememiştir. Bu araştırma akciğer (A549) ve meme kanseri (MDA-MB-231) hücre hatlarında sodyum fenilpirüvatın antitümöral etkilerinin belirlenmesi amacıyla yapılmıştır. Araştırmada distile suyla sodyum fenilpirüvatın değişik yoğunluklarda çözeltileri hazırlandı. Bu çözeltilerden içerisinde 1x103 adet kanser hücresi bulunan mikropleyt kuyucuklarına sırasıyla kontrol, 0.0375 mM, 0.075 mM, 0.15 mM, 0.3 mM, 0.6 mM, 1.2 mM ve 2.4 mM’lik konsantrasyonlarından 100 mikrolitre hacimlerde ilave edildi. Aynı doz sağlıklı hücrelere de uygulandı. Her kuyucuğa 24, 48 ve 72. saatlerde CVDK-8 Cell Viability Test kitinden (Eco-Tech) 10’ar µL eklendi ve 1 saat sonra mikropleytlerin 450 nm dalga boyunda verdikleri absorbansları spektrofotometrede ölçülerek hücrelerin yoğunlukları belirlendi. Kontrol ve deney gruplarından elde edilen sonuçlar istatistiki yönden değerlendirildi. Deney gruplarda kontrole göre kanser hücre sayılarının azaldığı, hücre katlanma sayılarının ise arttığı tespit edildi. Kontrole göre en fazla azalma Akciğer Karsinom hücre hattında 2,4 mM dozda 24. saatte, Meme Adenokarsinom hücre hattında 2,4 mM dozda 72. saatte ve Human Umbilical Vein Endothelial Cells (HUVEC) hattında 1,2 mM dozda 48. saatte olduğu tespit edildi. Sodyum fenilpirüvatın akciğer ve meme kanseri hücreleri üzerine in vitro şartlarda antitümöral etki gösterdiği sonucuna varıldı.

Keywords

Lung cancer, breast cancer, phenylpyruvic acide sodium salt

Thanks

Acknowledgements: Thanks to Dr. Barış Yıldız for experimental applications and statistical calculations.

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