Protective Effects of Montelukast Against Stress-Induced Degeneration of the Urinary Bladder

Year 2018, Volume: 8 Issue: 3, 211 - 216, 28.09.2018

Esra Çikler Dülger , Yasemin Ersoy Çanıllıoğlu Şule Çetinel Göksel Şener Feriha Ercan

https://doi.org/10.5152/clinexphealthsci.2017.691

Cited By: 1

Abstract

Objective: The aim of the study was to investigate the role of montelukast (ML), a

cysteinyl leukotriene-1 receptor antagonist, on the water avoidance stress (WAS) –

induced degeneration of the rat urinary bladder mucosa.

Methods: In WAS group, rats were exposed to WAS two hours daily for five days.

In WAS+ML group, rats were administered ML (10 mg/kg; i.p.;) following every WAS

exposure for 5 days. In control group, rats were injected vehicle solution only. The

urinary bladder was evaluated for general morphology at light microscope. Mast

cell activation and uroplakin distribution were assassed with immunohistochemistry.

Glycosaminoglycan (GAG) distribution and urothelial permeability were observed

using ruthenium red (RR) staining techniques in transmission electron microscope

and luminal urothelial cells were observed with scanning electron microscope. Tissue

malondialdehyde (MDA) and gluthatione (GSH) levels were also analysed.

Results: Irregular GAG layer and uroplakin distribution, penetration of RR in

the intercellular spaces and dilated tight junctions in urothelial layer, increase in

inflammatory cell infiltration, in number of both granulated and activated mast cells,

and were observed in the WAS group. The MDA level was increased, and GSH level

was decreased significantly in urinary bladder in the WAS group in comparison with

the control group. Quite regular GAG layer, uroplakin distribution and tight junctions

in most regions, decrease in inflammatory cell infiltration and both of activated and

granulated mast cells in the mucosa, were observed in WAS+ML group. Moreover,

significant decrease in MDA and increase in GSH levels were observed in this group.

Conclusion: Montelukast appears to exert a protective activity in WAS induced

urinary bladder injury by inhibiting inflammatory and oxidative activity.

Keywords

Water avoidance stress, montelukast, urinary bladder, uroplakin, mast cell

References

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