Naringenin Reduces Hepatic Inflammation and Apoptosis Induced by Vancomycin in Rats

Year 2021, Volume: 11 Issue: 2, 191 - 198, 30.06.2021

Zuhal Uçkun Şahinoğulları , Sevda Güzel , Necmiye Canacankatan , Cem Yalaza , Deniz Kibar Gulsen Bayrak

https://doi.org/10.33808/clinexphealthsci.741916

Cited By: 1

Abstract

Objective: This investigation aimed to detect the possible protective impacts of naringenin (NAR) on vancomycin (VCM)-induced liver toxicity through measuring caspase-3, -8 and -9 activities as markers of apoptosis and the levels of tumor necrosis factor-alpha, cyclooxygenase-2 and vascular endothelial growth factor as inflammation markers and assessing the histopathological alterations in rats.

Methods: The rats were allocated into seven groups as, the control group (saline, intraperitoneally (i.p.)), VCM group (400 mg/kg/day, i.p.), Carboxymethyl cellulose group (0.5% CMC, orally), NAR100 group (100 mg/kg/day, orally), VCM+NAR25 group (25 mg/kg/day, orally), VCM+NAR50 group (50 mg/kg/day, orally), VCM+NAR100 group (100 mg/kg/day, orally). The caspase enzyme activities and inflammation markers were measured using colorimetric methods and ELISA, respectively. Histopathological examinations were performed.

Results: The caspase activities and levels of inflammation markers were significantly higher in the VCM group as opposed to the other groups. The caspase activities were significantly ameliorated in the VCM+NAR25 group compared to the VCM+NAR50 and VCM+NAR100 groups, but the levels of inflammation markers were significantly attenuated in VCM+NAR50 group and, especially, VCM+NAR100 group compared to VCM+NAR25 group.

Conclusion: NAR has potential protective impact on liver injury caused by VCM, and the protective impacts of NAR at distinct doses may occur via different molecular mechanisms.

Keywords

Vancomycin, Naringenin, Liver, Apoptosis, Inflammation

Project Number

This study was supported by the Research Fund of Mersin University in Turkey with Project Number 2016-2-AP3-1906.

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