Antimicrobial Effect of Polyhexanide on Denture Base and Soft Lining Materials

Year 2022, Volume: 12 Issue: 1, 113 - 119, 30.03.2022

Doğukan Yılmaz , Hakan Akın

https://doi.org/10.33808/clinexphealthsci.833576

Abstract

Objective: Polyhexanide (PHMB; polyhexamethylene biguanide) is a well-known antiseptic agent; however, no data exist for its application
on denture base and lining materials. In the present in vitro study, the aim was to compare viable bacterial or fungal cell numbers and their
adhesion on different types of denture base and lining materials in diverse concentrations of PHMB.
Methods: Light-activated urethane dimethacrylate (UDMA), heat-polymerized polymethyl methacrylate (PMMA), heat-polymerized
polydimethylsiloxane, and autopolymerizing polyethylmethacrylate discs were prepared for each group (n = 10). 1×108 CFU/mL of all the tested
species were appended separately to discs, and they were immersed into different PHMB suspensions (0.5%, 1%, 2%, and 5%) for 10 minutes.
The antimicrobial activity and number of adherent species on the surface were evaluated.
Results: In the PMMA group, all studied species except C. albicans, L. acidophilus, and S. aureus were decreased in various concentrations (p <
0.05), and all studied species presented a significant decrease in every concentration of PHMB in the UDMA group (p < 0.01) in comparison to the
control. N. sicca, K. pneumoniae, S. pyogenes, S. sanguis, C. pseudotuberculosis, and S. aureus (p < 0.05) were reduced in the heat-polymerized
polydimethylsiloxane group, while all tested species except B. subtilis were decreased in the autopolymerizing polyethylmethacrylate group in
comparison to the control (p < 0.01). Among all tested materials and species, no significant difference was detected in adherent cell number
(p > 0.05).
Conclusion: PHMB suspension, in various concentrations, can reduce some species of bacterial and yeast cells.

Keywords

Disinfectant, bacterial growth, denture adhesive, denture base

Supporting Institution

None to declare

References

• [1] Kawala M, Smardz J, Adamczyk L, Grychowska N, Wieckiewicz M. Selected applications for current polymers in prosthetic dentistry - state of the art. Curr Med Chem. 2018;25:6002-6012.
• [2] Coulthwaite L, Verran J. Potential pathogenic aspects of denture plaque. Br J Biomed Sci. 2007;64:180-189.
• [3] Kang SH, Lee HJ, Hong SH, Kim KH, Kwon TY. Influence of surface characteristics on the adhesion of Candida albicans to various denture lining materials. Acta Odontol Scand. 2013;71:241-248.
• [4] Gomes AS, Sampaio-Maia B, Vasconcelos M, Fonesca PA, Figueiral H. In situ evaluation of the microbial adhesion on a hard acrylic resin and a soft liner used in removable prostheses. Int J Prosthodont. 2015;28:65-71.
• [5] Teughels W, Van Assche N, Sliepen I, Quirynen M. Effect of material characteristics and/or surface topography on biofilm development. Clin Oral Implants Res. 2006;17:68-81.
• [6] Busscher HJ, Weerkamp AH, van der Mei HC, van Pelt AW, de Jong HP, Arends J. Measurement of the surface free energy of bacterial cell surfaces and its relevance for adhesion. Appl Environ Microbiol. 1984;48:980-983.
• [7] Gendreau L, Loewy ZG. Epidemiology and etiology of denture stomatitis. J Prosthodont. 2011;20:251-260.
• [8] Pellizzaro D, Polyzois G, Machado AL, Giampaolo ET, Sanitá PV, Vergani CE. Effectiveness of mechanical brushing with different denture cleansing agents in reducing in vitro Candida albicans biofilm viability. Braz Dent J. 2012;23:547-554.
• [9] Budtz-Jørgensen E. Materials and methods for cleaning dentures. J Prosthet Dent. 1979;42:619-623.
• [10] Altieri KT, Sanitá PV, Machado AL, Giampaolo ET, Pavarina AC, Vergani CE. Effectiveness of two disinfectant solutions and microwave irradiation in disinfecting complete dentures contaminated with methicillin-resistant Staphylococcus aureus. J Am Dent Assoc. 2012;143:270-277.
• [11] Duyck J, Vandamme K, Muller P, Teughels W. Overnight storage of removable dentures in alkaline peroxide-based tablets affects biofilm mass and composition. J Dent. 2013;41:1281-1289.
• [12] Mota AC, de Castro RD, de Araújo Oliveira J, de Oliveira Lima E. Antifungal activity of apple cider vinegar on Candida species involved in denture stomatitis. J Prosthodont. 2015;24:296-302.
• [13] Andonissamy L, Karthigeyan S, Ali SA, Felix JW. Effect of Chemical denture disinfectants and tree extracts on biofilm-forming Staphylococcus aureus and viridans Streptococcus species isolated from complete denture. J Contemp Dent Pract. 2019;20:1307-1314.
• [14] Soto AF, Mendes EM, Arthur RA, Negrini TC, Lamers ML, Mengatto CM. Antimicrobial effect and cytotoxic activity of vinegar-hydrogen peroxide mixture: A possible alternative for denture disinfection. J Prosthet Dent. 2019;121:966.e1-966.e6.
• [15] Masetti P, Arbeláez MIA, Pavarina AC, Sanitá PV, Jorge JH. Cytotoxic potential of denture base and reline acrylic resins after immersion in disinfectant solutions. J Prosthet Dent. 2018;120:155.e1-155.e7.
• [16] Chidambaranathan AS, Balasubramanium M. Comprehensive review and comparison of the disinfection techniques currently available in the literature. J Prosthodont. 2019;28:849-856.
• [17] Sowlati-Hashjin S, Carbone P, Karttunen M. Insights into the polyhexamethylene biguanide (PHMB) mechanism of action on bacterial membrane and DNA: A molecular dynamics study. J Phys Chem B. 2020;124:4487-4497.
• [18] Sibbald RG, Coutts P, Woo KY. Reduction of bacterial burden and pain in chronic wounds using a new polyhexamethylene biguanide antimicrobial foam dressing-clinical trial results. Adv Skin Wound Care. 2011;24:78-84.
• [19] Romanowski EG, Yates KA, O'Connor KE, Mah FS, Shanks RM, Kowalski RP. Evaluation of polyhexamethylene biguanide (PHMB) as a disinfectant for adenovirus. JAMA Ophthalmol. 2013;131:495-498.
• [20] Szkołuda P, Karpiński TM. Polyhexanide (PHMB)–properties and applications in medicine. Eur J Biol Res 2020;10:225-231.
• [21] Hübner NO, Kramer A. Review on the efficacy, safety and clinical applications of polihexanide, a modern wound antiseptic. Skin Pharmacol Physiol. 2010;23:17-27.
• [22] Rosin M, Welk A, Bernhardt O, Ruhnau M, Pitten FA, Kocher T, Kramer A. Effect of a polyhexamethylene biguanide mouthrinse on bacterial counts and plaque. J Clin Periodontol. 2001;28:1121-1126.
• [23] Welk A, Splieth CH, Schmidt-Martens G, Schwahn Ch, Kocher T, Kramer A, Rosin M. The effect of a polyhexamethylene biguanide mouthrinse compared with a triclosan rinse and a chlorhexidine rinse on bacterial counts and 4-day plaque re-growth. J Clin Periodontol. 2005;32:499-505.
• [24] Yu X, Jiang W, Shi Y, Ye H, Lin J. Applications of sequencing technology in clinical microbial infection. J Cell Mol Med. 2019;23:7143-7150.
• [25] Budtz-Jörgensen E, Theilade E, Theilade J. Quantitative relationship between yeast and bacteria in denture-induced stomatitis. Scand J Dent Res. 1983;91:134-142.
• [26] Koburger T, Hübner NO, Braun M, Siebert J, Kramer A. Standardized comparison of antiseptic efficacy of triclosan, PVP-iodine, octenidine dihydrochloride, polyhexanide and chlorhexidine digluconate. J Antimicrob Chemother. 2010;65:1712-1719.
• [27] Wessels S, Ingmer H. Modes of action of three disinfectant active substances: a review. Regul Toxicol Pharmacol. 2013;67:456-467.
• [28] Yanai R, Ueda K, Nishida T, Toyohara M, Mori O. Effects of ionic and surfactant agents on the antimicrobial activity of polyhexamethylene biguanide. Eye Contact Lens. 2011;37:85-89.
• [29] Ceviker K, Canikoglu M, Tatlıoglu S, Bagdatli Y. Reducing the pathogen burden and promoting healing with polyhexanide in non-healing wounds: a prospective study. J Wound Care. 2015;24:582-586.
• [30] Morse DJ, Smith A, Wilson MJ, Marsh L, White L, Posso R, Bradshaw DJ, Wei X, Lewis MAO, Williams DW. Molecular community profiling of the bacterial microbiota associated with denture-related stomatitis. Sci Rep. 2019;9:10228.