Effect of Different Mouthwashing Regimens on Adhesion of a Universal Adhesive:A Microshear Bond Strength and Scaning Electron Microscopy Evaluation

Year 2022, Volume: 12 Issue: 4, 919 - 925, 30.12.2022

Ece Meral , Cansu Atalay , Esra Ergin

https://doi.org/10.33808/clinexphealthsci.993200

Abstract

Objective: To evaluate the effect of probiotic or chlorhexidine-based mouthwashes and coconut oil pulling therapy on microshear bond strength of a universal adhesive, used with two application modes. Method: Ninety-six enamel specimens were prepared using bovine incisors and the surfaces were grounded. Then the specimens were randomly divided into 4groups and each group were subjected to a mouth washing regimen with one of three agents-chlorhexidine mouthwash, probiotic-based mouthwash, coconut oil pulling – or stored in artificial saliva(control) for 7 days(n=24). After the procedure, all groups were divided into 2 subgroups, and a universal adhesive was applied with etch-and-rinse or self-etch mode(n=12). Composite micro-cylinders were bonded to the enamel surfaces and micro-shear-bond strength was measured after 24hours water storage. Failure modes were determined using a stereomicroscope and SEM analysis was also performed. The data were analyzed using Mann-Whitney-U and Kruskal-Wallis tests. Results: No significant differences were observed between the different mouthwash groups, regardless of application modes(p> .05). There were no significant differences in microshear-bond strength, within the same mouthwash groups, between self-etch or etch-and-rinse modes, except for oil pulling group. Etch-and-rinse group showed higher bond strength than self-etch group in specimens subjected to oil pulling(p< .05). Conclusion: Etch-and-rinse mode might be preferable on patients who practice oil pulling.

Keywords

oil pulling, universal adhesive, shear-bond strength, mouthwash

References

• [1] Kandaswamy SK, Sharath A, Priya PG. Comparison of the Effectiveness of Probiotic, Chlorhexidine-based Mouthwashes, and Oil Pulling Therapy on Plaque Accumulation and Gingival Inflammation in 10 – to 12-year-old Schoolchildren: A Randomized Controlled Trial. Int J Clin Pediatr Dent. 2018;11(2):66-70. DOI: 10.5005/jp-journals-10005-1487
• [2] Derafshi R, Khorshidi H, Kalantari M, Ghaffarlou I. Effect of mouthrinses on color stability of monolithic zirconia and feldspathic ceramic: an in vitro study. BMC Oral Health. 2017;17(1):129. DOI: 10.1186/s12903.017.0419-9
• [3] Stamatova I, Meurman JH. Probiotics: health benefits in the mouth. Am J Dent. 2009;22(6):329-338.
• [4] Nase L, Hatakka K, Savilahti E, Saxelin M, Ponka A, Poussa T. Effect of long-term consumption of a probiotic bacterium, Lactobacillus rhamnosus GG, in milk on dental caries and caries risk in children. Caries Res. 2001;35(6):412-420. DOI: 10.1159/000047484
• [5] Kang MS, Kim BG, Chung J, Lee HC, Oh JS. Inhibitory effect of Weissella cibaria isolates on the production of volatile sulphur compounds. J Clin Periodontol. 2006;33(3):226-232. DOI: 10.1111/j.1600-051X.2006.00893.x
• [6] Shanbhag VK. Oil pulling for maintaining oral hygiene – A review. J Tradit Complement Med. 2017;7(1):106-109. DOI: 10.1016/j.jtcme.2016.05.004
• [7] Nagarkar S, Theis-Mahon N, Perdigão J. Universal dental adhesives: Current status, laboratory testing, and clinical performance. Journal of Biomedical Materials Research Part B: Applied Biomaterials. 2019;107(6):2121-231. DOI: 10.1002/ jbm.b.34305
• [8] Diniz A, Bandeca MC, Pinheiro LM, Dos JSAL, Torres C, Borges AH. Influence of Different Etching Modes on Bond Strength to Enamel using Universal Adhesive Systems. The journal of contemporary dental practice. 2016;17(10):820-825. DOI: 10.5005/jp-journals-10024-1937
• [9] Beltrami R, Chiesa M, Scribante A, Allegretti J, Poggio C. Comparison of shear bond strength of universal adhesives on etched and nonetched enamel. Journal of Applied Biomaterials & Functional Materials. 2016;14(1):78-83. DOI: 10.5301/jabfm.5000261
• [10] Suda S, Tsujimoto A, Barkmeier WW, Nojiri K, Nagura Y, Takamizawa T. Comparison of enamel bond fatigue durability between universal adhesives and two-step self-etch adhesives: effect of phosphoric acid pre-etching. Dental materials journal. 2018;37(2):244-255. DOI: 10.4012/dmj.2017-059
• [11] Lawson NC, Robles A, Fu C-C, Lin CP, Sawlani K, Burgess JO. Twoyear clinical trial of a universal adhesive in total-etch and selfetch mode in non-carious cervical lesions. Journal of dentistry. 2015;43(10):1229-1234. DOI: 10.1016/j.jdent.2015.07.009
• [12] Armstrong S, Geraldeli S, Maia R, Raposo LH, Soares CJ, Yamagawa J. Adhesion to tooth structure: a critical review of “micro” bond strength test methods. Dent Mater. 2010;26(2):e50-62. DOI: 10.1016/j.dental.2009.11.155
• [13] Shah S. S, Nambiar S, Kamath D, Suman E, Unnikrishnan B, Desai A, Mahajan S, Dhawan, K. Comparative evaluation of plaque inhibitory and antimicrobial efficacy of probiotic and chlorhexidine oral rinses in orthodontic patients: a randomized clinical trial. International journal of dentistry, 2019:1-6. DOI: 10.1155/2019/1964158
• [14] Sinha DJ, Jaiswal N, Vasudeva A, Garg P, Tyagi SP, Chandra P. Comparative evaluation of the effect of chlorhexidine and Aloe barbadensis Miller (Aloe vera) on dentin stabilization using shear bond testing. J Conserv Dent. 2016;19(5):406-409. DOI: 10.4103/0972-0707.190017
• [15] K Suma N, K Shashibhushan K, Subba Reddy VV. Effect of Dentin Disinfection with 2% Chlorhexidine Gluconate and 0.3% Iodine on Dentin Bond Strength: An in vitro Study. Int J Clin Pediatr Dent. 2017;10(3):223-228. DOI: 10.5005/jpjournals-10005-1440
• [16] Frey C, Yetkiner E, Stawarczyk B, Attin T, Attin R. Effects of different chlorhexidine pretreatments on adhesion of metal brackets in vitro. Head Face Med. 2012;8:36. DOI: 10.1186/1746-160X-8-36
• [17] Bishara SE, Damon PL, Olsen ME, Jakobsen JR. Effect of applying chlorhexidine antibacterial agent on the shear bond strength of orthodontic brackets. Angle Orthod. 1996;66(4):313-316.
• [18] Bishara SE, Vonwald L, Zamtua J, Damon PL. Effects of various methods of chlorhexidine application on shear bond strength. American Journal of Orthodontics and Dentofacial Orthopedics. 1998;114(2):150-153. DOI: 10.1053/od.1998. v114.a84780
• [19] Comelli EM, Guggenheim B, Stingele F, Neeser JR. Selection of dairy bacterial strains as probiotics for oral health. Eur J Oral Sci. 2002;110(3):218-24. DOI: 10.1034/j.1600-0447.2002.21216.x
• [20] Sookkhee S, Chulasiri M, Prachyabrued W. Lactic acid bacteria from healthy oral cavity of Thai volunteers: inhibition of oral pathogens. J Appl Microbiol. 2001;90(2):172-9. DOI: 10.1046/j.1365-2672.2001.01229.x
• [21] Ahola AJ, Yli-Knuuttila H, Suomalainen T, Poussa T, Ahlstrom A, Meurman JH. Short-term consumption of probiotic-containing cheese and its effect on dental caries risk factors. Arch Oral Biol. 2002;47(11):799-804. DOI: 10.1016/S0003-9969(02)00112-7
• [22] Deshmukh MA, Dodamani AS, Karibasappa G, Khairnar MR, Naik RG, Jadhav HC. Comparative evaluation of the efficacy of probiotic, herbal and chlorhexidine mouthwash on gingival health: A randomized clinical trial. J Clin Diagn Res. 2017;11(3):ZC13-ZC6. DOI: 10.7860/JCDR/2017/23891.9462
• [23] Harini P, Anegundi R. Efficacy of a probiotic and chlorhexidine mouth rinses: A short-term clinical study. Journal of Indian society of pedodontics and preventive dentistry. 2010;28(3):179. DOI: 10.4103/0970-4388.73799
• [24] Purunaik S, Thippeswamy H, Chavan SS. To evaluate the effect of probiotic mouthrinse on plaque and gingivitis among 1516 year old school children of Mysore City, India-randomized controlled trial. Glob J Med Res. 2014;14:9-14.
• [25] Jayasheel A, Niranjan N, Pamidi H, Suryakanth MB. Comparative Evaluation of shear Bond Strength of universal Dental Adhesives – An in vitro study. J Clin Exp Dent. 2017;9(7):e892-e896. DOI:10.4317/jced.53816
• [26] Asokan S, Rathan J, Muthu MS, Rathna PV, Emmadi P, Raghuraman. Effect of oil pulling on Streptococcus mutans count in plaque and saliva using Dentocult SM Strip mutans test: a randomized, controlled, triple-blind study. J Indian Soc Pedod Prev Dent. 2008;26(1):12-17. DOI: 10.4103/09704388.40315
• [27] Parolia A. Oil hygiene. Br Dent J. 2009;207(9):408. DOI: 10.1038/sj.bdj.2009.965
• [28] Jacker-Guhr S, Sander J, Luehrs AK. How “Universal” is Adhesion? Shear Bond Strength of Multi-mode Adhesives to Enamel and Dentin. J Adhes Dent. 2019;21(1):87-95. DOI: 10.3290/j.jad.a41974
• [29] McLean DE, Meyers EJ, Guillory VL, Vandewalle KS. Enamel Bond Strength of New Universal Adhesive Bonding Agents. Oper Dent. 2015;40(4):410-417. DOI: 10.2341/13-287-L
• [30] Teruel Jde D, Alcolea A, Hernandez A, Ruiz AJ. Comparison of chemical composition of enamel and dentine in human, bovine, porcine and ovine teeth. Arch Oral Biol. 2015;60(5):768-775. DOI: 10.1016/j.archoralbio.2015.01.014
• [31] Perdigão J, Kose C, Mena-Serrano A, De Paula E, Tay L, Reis A. A New Universal Simplified Adhesive: 18-Month Clinical Evaluation. Oper Dent. 2014;39(2):113-127. DOI: 10.2341/13045-C