Does Chitosan Introduce Protection Against Methotrexate-Induced Hepatorenal Injury in Rats?

Year 2024, Volume: 14 Issue: 1, 39 - 44, 28.03.2024

Ahmet Özer Şehirli , Serkan Sayıner , Kani Bilginaylar , Hanife Özkayalar , Aslı Aykaç

https://doi.org/10.33808/clinexphealthsci.1134320

Abstract

Objective: Chitosan possesses antioxidant properties and exhibits anti-inflammatory characteristics. The objective of the investigation was to assess the effectiveness of chitosan in protecting against hepatorenal injury induced by methotrexate (MTX), a medication utilized for immunosuppression and chemotherapy.
Methods: Wistar albino rats were allocated into 3 different groups, each consisting of six animals (n=6). The control group received saline for 5 days (i.p.), the MTX group was administrated a single dose MTX (60 mg/kg, i.p.) along with saline for four days (i.p.), while MTX+Chitosan group received a single dose of MTX (60 mg/kg, i.p.) followed by Chitosan administration (200 mg/kg, i.p.) for four days. On the sixth day, the animals were decapitated, and blood and tissue samples were collected. BUN, creatinine and tissue inhibitors of metalloproteinase-1 (TIMP-1) levels and activities of AST, ALT, ALP, LDH, matrix metalloproteinases (MMP-3, MMP-8, MMP-9) activities were quantified in the blood. The liver and kidney were evaluated for caspase-3 and-9 through western blotting, while structural damage was examined using light microscopy.
Results: In the MTX administered group, blood and tissues values except for all TIMP-1 statistically increased when compared to the control group, while activity of TIMP-1 decreased significantly. The Chitosan-treated MTX group had comparable values to the control group.
Conclusion: Based on its influence on metalloproteinases and caspases, our findings lead to the conclusion that Chitosan offers a protective effect against liver and kidney damage induced by MTX.

Keywords

Caspase, Chitosan, Methotrexate, MMP, TIMP-1

Project Number

Yok

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