Evaluation of Traceability of Dietary Urine Biochemistry Changes with Commercial Urine Strips

Year 2024, Volume: 14 Issue: 2, 494 - 500, 28.06.2024

Gizem Çebi , Balım Bengisu Caf , Esra Kozan , Serap Derman , Haluk Çelik , Miray Tayfun

https://doi.org/10.33808/clinexphealthsci.1401209

Abstract

Objective: This research aims to (i) examine the effects of nutrition on urine biochemistry and (ii) compare the two different measurement methods (laboratory and commercial strip). This means it is desired to bring a new direction to the literature.
Methods: The study involved 42 women aged 20-30 from Turkey and examined the urinary excretion of calcium, vitamin C (smoker and non-smoker), sodium, and magnesium based on nutritional status. The collected urine samples were applied onto commercial urine strips, and the resulting color changes were recorded by smartphone; at the same time, it was sent to the laboratory for comparative analysis. The obtained data were used in regression and correlation statistical analysis. All statistical analyzes were performed using IBM SPSS 28.0.
Results: While evaluating the regression analysis results in which the excretion due to nutrition was examined, each nutritional level was compared to the restricted intake. ANOVA sig values <.001, t values 1.96 <, in all metabolites (calcium, vitamin C, sodium, magnesium) evaluations. The following rates (R2 values) were obtained restricted/optimal nutrition in calcium, vitamin C, sodium, and magnesium: .636, .575, .386, and .209 respectively; restricted/high nutrition in calcium, vitamin C (non-smoker), vitamin C (smoker), sodium, magnesium: .442, .308, .482,.413 and .337 respectively; restricted/supplement in calcium, vitamin C, magnesium: .273, .698 and .799. Calcium and magnesium strips correlated strongly with lab results, correlation coefficients are .679 and .59 respectively. Sodium and creatinine strips correlated very strongly with lab results, correlation coefficients are .876 and .884 respectively.
Conclusion: The study revealed that nutrition significantly affected urine excretion levels for calcium, vitamin C, sodium, and magnesium. Additionally, the results showed that urine strips had a correlation with laboratory results indicating their usefulness for pre-diagnosis purposes.

Keywords

nutrition, urine biochemistry, urinary excretion, commercial urine strip, statistical analyzes

References

• O’Sullivan A. Metabolic cross-talk between diet and health. Nat Food 1. 2020;398-399. DOI: 10.1038/s43016-020-0118-6
• Playdon MC, Moore SC, Derkach A, Reedy J, Subar AF, Sampson JN, Albanes D, Gu F, Kontto J, Lassale C, Liao LM, Männistö S, Mondul AM, Weinstein SJ, Irwin ML, Mayne ST, Stolzenberg-Solomon R. Identifying biomarkers of dietary patterns by using metabolomics. Am J Clin Nutr. 2017;105(2):450-465. DOI: 10.3945/ajcn.116.144501
• Posma JM, Garcia-Perez I, Frost G, Aljuraiban GS, Chan Q, Van Horn L, Daviglus M, Stamler J, Holmes E, Elliott P, Nicholson JK. Nutriome–metabolome relationships provide insights into dietary intake and metabolism. Nat Food 1. 2020;426-436. DOI: 10.1038/s43016-020-0093-y
• Lloyd AJ, Willis ND, Wilson T, Zubair H, Xie L, Chambers E, Garcia-Perez I, Tailliart K, Beckmann M, Mathers JC, Draper J. Developing a food exposure and urine sampling strategy for dietary exposure biomarker validation in free-living individuals. Mol Nutr Food Res. 2019;63(14):e1900062. DOI: 10.1002/mnfr.201900062
• Farquhar WB, Edwards DG, Jurkovitz CT, Weintraub WS. Dietary sodium and health: More than just blood pressure. J Am Coll Cardiol. 2015;65(10):1042-1050. DOI: 10.1016/j.jacc.2014.12.039
• Shkembi B, Huppertz T. Calcium absorption from food products: food matrix effects. Nutrients. 2021;14(1):180. DOI: 10.3390/nu14010180
• Zhang Z, Liu J, Cheng Y, Chen J, Zhao H, Ren X. Urine analysis has a very broad prospect in the future. Front Anal Sci. 2022;1, 812301.DOI: 10.3389/frans.2023.1118749
• Çelik H, Caf BB, Geyik C, Çebi G, Tayfun M. Enhancing urinalysis with smartphone and AI: A comprehensive review of point-of-care urinalysis and nutritional advice. Chem. Pap. 2024;78: 651–664. DOI: 10.1007/s11696-023-03137-z
• Carroll MF, Temte JL. Proteinuria in adults: A diagnostic approach. Am fam physician. 2000;62(6):1333-1340.
• Barratt J, Topham P. Urine proteomics: The present and future of measuring urinary protein components in disease. Cmaj. 2007;177(4), 361-368. DOI: 10.1503/cmaj.061590
• Wójcik M, Kozioł-Kozakowska A. Obesity, Sodium Homeostasis, and Arterial Hypertension in Children and Adolescents. Nutrients. 2021;13(11):4032. DOI: 10.3390/nu13114032
• Vannucci L, Fossi C, Quattrini S, Guasti L, Pampaloni B, Gronchi G, Giusti F, Romagnoli C, Cianferotti L, Marcucci G, Brandi ML. Calcium ıntake in bone health: A focus on calcium-rich mineral waters. Nutrients. 2018;10(12):1930. DOI:10.3390/nu10121930
• Figueroa-Méndez R, Rivas-Arancibia S. Vitamin C in health and disease: Its role in the metabolism of cells and redox state in the brain. Front. Physiol. 2015;6:397. DOI: 10.3389/fphys.2015.00397
• Jafari D, Esmaeilzadeh A, Mohammadi-Kordkhayli M, Rezaei, N. Vitamin C and the immune system. Springer, Cham. 2019. DOI: 10.1007/978-3-030-16073-9_5
• Pullar JM, Carr AC, Vissers MCM. The roles of vitamin C in skin health. Nutrients 2017; 9(8):866. DOI: 10.3390/nu9080866
• Carr AC, Maggini S. Vitamin C and immune function. Nutrients 2017;9(11), 1211; DOI: 10.3390/nu9111211
• Strazzullo P, Leclercq C. Sodium. Adv Nutr. 2014;5(2):188-90. DOI: 10.3945/an.113.005215.
• Jahnen-Dechent W, Ketteler M. Magnesium basics. Clin Kidney J. 2012;Volume 5, Pages i3–i14. DOI: 10.1093/ndtplus/sfr163
• Barbagallo M, Dominguez LJ. Magnesium and aging. Curr Pharm Des. 2010;Volume 16, Number 7. DOI: 10.2174/138161210790883679
• Gröber U, Schmidt J, Kisters K. Magnesium in prevention and therapy. Nutrients 2015;7(9):8199-8226; DOI: 10.3390/nu7095388
• National Institutes of Health. Nutrient Recommendations and Databases. Accessed [July 2022]. https://ods.od.nih.gov/HealthInformation/nutrientrecommendations.aspx
• Institute of Medicine (US) Committee to Review Dietary Reference Intakes for Vitamin D and Calcium; Ross AC, Taylor CL, Yaktine AL, Del Valle HB. Dietary Reference Intakes for Calcium and Vitamin D. Washington (DC): National Academies Press (US); 2011. DOI: 10.17226/13050
• Traber MG, Stevens JF. Vitamins C and E: Beneficial effects from a mechanistic perspective. Free Radic Biol Med. 2011;51(5):1000-1013. DOI: 10.1016/j.freeradbiomed.2011.05.017
• Chambial S, Dwivedi S, Shukla KK, John PJ, Sharma P. Vitamin C in disease prevention and cure: an overview. Indian J Clin Biochem. 2013;28:314-328. DOI: 10.1007/s12291-013-0375-3.
• Whiting P, Westwood M, Watt I, Cooper J, Kleijnen J. Rapid tests and urine sampling techniques for the diagnosis of urinary tract infection (UTI) in children under five years: a systematic review. BMC Pediatr. 2005;5(1):4. DOI: 10.1186/1471-2431-5-4
• Oyaert M, Delanghe JR. Semiquantitative, fully automated urine test strip analysis. J Clin Lab Anal. 2019;33(5):e22870. DOI: 10.1002/jcla.22870
• Shojaei-far Z, Razi F, Bandarian F, Rambod C, Qorbani M. A Detailed Comparison of Morning and Random Urine Specimen Levels with 24 Hour Urinary Excretion Levels of Seven Biochemic. Ann Clin Lab Sci. 2017;47(2):201-207.
• Dong Y, Silver SM, Sterns R. Estimating urine volume from the urine creatinine concentration. Nephrol Dial Transplant. 2023;38(4):811-818. DOI: 10.1093/ndt/gfab337