Genotypic and Phenotypic Evaluation of Heavy Metal Resistance of Enterococcal Isolates from Seafood Products for Consumption

Year 2023, , 58 - 64, 30.06.2023

Mine Çardak , Sine Özmen Toğay , Mustafa Ay

https://doi.org/10.31594/commagene.1250775

Abstract

The aim of this study was to genotypically and phenotypically evaluate the resistance to heavy metal salts of enterococci isolated from fisheries sold in our country's market for food safety. Using concentrations computed as 1000 ppm of seven heavy metal salts, copper (Cu+2), nickel (Ni+2), cadmium (Cd+2), zinc (Zn+2), lead (Pb+2), chromium (Cr+2), and iron (Fe+2) heavy metal resistance (MIK) tests were conducted on each of the enterococci isolates. As a result of the analyses made in this context, resistance to heavy metal salts such as copper, lead, nickel, and cadmium in many isolates and the presence of copper (tcrB) and cadmium (cadA) resistance genes in some isolates were determined. It is considered that the isolates and the fishery products from which these isolates were obtained may carry risks in terms of food safety and public health. In addition, genes encoding heavy metal resistance are also effective in antibiotic resistance. For this reason, it is stated that these microorganisms gain importance not only in applications such as bioremediation and biomining but also in the healthcare sector.

Keywords

Enterococci, seafood, heavy, metal, resistance

Supporting Institution

TUBİTAK

Project Number

215O374

Thanks

: This work was supported by the Scientific and Technological Research Council of Turkey (TUBITAK) under the project number 215O374. We would like to thank Prof. Dr. Gülşen ALTUĞ to have assisting us in the identification of bacterial isolates with the bacterial automation device VITEK 2 Compact System.

Tüketime Sunulan Su Ürünlerinde Enterokok İzolatlarında Ağır Metal Direncinin Genotipik ve Fenotipik Olarak Değerlendirilmesi

Abstract

Bu çalışmanın amacı, ülkemiz piyasasında satışa sunulan su ürünlerinden izole edilen enterokokların gıda güvenliğine uygunluğunun ağır metal tuzlarına dirençlilik özelliklerini genotipik ve fenotipik olarak değerlendirmektedir. Enterokok izolatlarının her biri için bakır (Cu+2), nikel (Ni+2), kadmiyum (Cd+2), çinko (Zn+2), kurşun (Pb+2), krom (Cr+2) ve demir (Fe+2) olmak üzere 7 adet ağır metal tuzları 1000 ppm olarak hesaplanan konsantrasyonlar kullanılarak ağır metal dirençlilik (MIK) testleri yapılmıştır. Bu kapsamda yapılan analizler sonucunda test edilen pek çok izolatlarda bakır, kurşun, nikel, kadmiyum gibi ağır metal tuzlarına karşı direnç özellikleri ve bazı izolatlarda bakır (tcrB) ve kadmiyum (cadA) direnç genlerinin varlığı da belirlenmiştir. İzolatların ve bu izolatların elde edildiği su ürünü örneklerinin gıda güvenliği ve halk sağlığı yönüyle risk taşıyabileceği düşünülmüştür. Bunun yanında ağır metal dirençliliğini kodlayan genler aynı zamanda antibiyotik dirençliliğinde de etkilidir. Bu nedenle bu mikroorganizmalar sadece biyoremediasyon, biyomadencilik gibi uygulamalar için değil sağlık sektöründe de önem kazandığı belirtilmiştir.

Keywords

Bakteri, enterokok, su ürünü, ağır metal direnci, gıda güvenliği

Project Number

215O374

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