Erythrocytic and Nuclear Abnormalities in Natrix sp. from the Biga Stream (Çanakkale, Türkiye)

Year 2024, Volume: 8 Issue: 1, 30 - 36, 30.06.2024

Nurşen Çördük , Begüm Boran , Bengi Baycan , İbrahim Uysal

https://doi.org/10.31594/commagene.1461253

Abstract

The Grass Snake (Natrix natrix) and the Dice Snake (Natrix tessellata) are potential bioindicator water snake species in certain aquatic habitats. The aim of this study is to evaluate the frequency of erythrocytic abnormalities, immature erythrocyte index, and nuclear abnormalities in Natrix natrix and Natrix tessellata from Biga Stream in Çanakkale, Türkiye. Erythrocytic abnormalities and nuclear abnormalities such as micronuclei, lobbed nuclei, notched nuclei, blebbed nuclei, and binucleate cells were observed in peripheral blood smears for each sample. According to our results, total erythrocytic abnormalities in N. natrix (3.01±0.55%) were higher than in N. tessellata (2.52±0.32%). Similarly, the frequency of total nuclear abnormalities in N. natrix (13.40±2.7%) was higher than in N. tessellata (12.68±5.81%) but there was no statistically significant difference in total erythrocytic and nuclear abnormalities. However, student's t-test revealed that there was a statistically significant difference between the two species only in terms of immature erythrocyte index (p: 0.008) which was higher in N. natrix. Due to the results, it was suggested that N. natrix may exhibit more physiological responses to envirenmental stress factors.

Keywords

Grass snake, dice snake, micronucleus test, ecotoxicology

Ethical Statement

The study was conducted with the permission of Çanakkale Onsekiz Mart University, Animal Experiments Local Ethics Committee (Permit no. 2017/04-16).

Biga Çayı (Çanakkale, Türkiye)’nda Dağılış Gösteren Natrix sp.’lerin Eritrositik ve Nüklear Anormallikleri

Abstract

Yarı Sucul Yılan (Natrix natrix) ve Su Yılanı (Natrix tessellata), belirli sucul habitatlarda potansiyel biyoindikatör olan yılan türleridir. Bu çalışmanın amacı, Biga Çayı’nda (Çanakkale, Türkiye) bulunan Natrix natrix ve Natrix tessellata türlerinin eritrositik anormallik frekansı, olgunlaşmamış eritrosit indeksi ve nüklear anormallik frekansının belirlenmesidir. Her bir örnek için periferik kan yaymalarında, eritrositik anormallikler ve mikronükleus, loblu nükleus, çentikli nükleus, tomurcuklu nükleus, binükleat hücre gibi nüklear anormallikler gözlenmiştir. Sonuçlarımıza göre, N. natrix’teki toplam eritrositik anormallikler (%3.01±0.55) N. tessellata’dan (%2.52±0.32) daha yüksektir. Aynı şekilde N. natrix’teki toplam nüklear anormallik frekansının (%13.40±2.7) da N. tessellata’dan (%12.68±5.81) daha yüksek olduğu tespit edilmiştir fakat toplam eritrositik ve nüklear anormallikler bakımından istatistiksel olarak anlamlı bir fark bulunmamıştır. Ancak, student t testi ile iki tür arasında sadece olgunlaşmamış eritrosit indeksi (p: 0.008) açısından istatistiksel olarak anlamlı farklılık olduğu, N. natrix’de daha yüksek olduğu ortaya konmuştur. Bu sonuçlar doğrultusunda N. natrix’in çevresel stres faktörlerine karşı daha fazla fizyolojik tepkiler gösterebileceği düşünülmektedir.

Keywords

Yarı sucul yılan, su yılanı, mikronükleus testi, ekotoksikoloji

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