Ruminal Ciliate (Ciliophora, Trichostomatia) Biota of European Roe Deer (Capreolus capreolus) in Kastamonu

Year 2024, Volume: 8 Issue: 1, 46 - 50, 30.06.2024

Gözde Gürelli , Ekrem Mutlu

https://doi.org/10.31594/commagene.1487597

Abstract

This study investigated the ruminal ciliate biota of European roe deer (Capreolus capreolus) in Kastamonu. Only one species, Entodinium dubardi (Ophryoscolecidae, Trichostomatia), was identified from the ruminal contents of twelve (70.6% prevalence) of seventeen roe deers. Five of seventeen roe deer had no ciliates. E. dubardi was determined for the first time from the ruminal content of roe deer in Türkiye. The average ciliate concentration was 17.5 ± 27.0 × 104 cells mL–1. Min.–max. values ranged from 0 to 87 × 104 cells mL–1.

Keywords

Cervidae, Entodinium dubardi, Ophryoscolecidae, microorganism, ecosystem

Thanks

We thank the Ministry of Agriculture and Forestry Kastamonu Provincial Directorate staff for their sampling assistance and Kastamonu University Central Research Laboratory for SEM images.

Kastamonu’daki Avrupa Karacalarının (Capreolus capreolus) İşkembe Siliyat (Ciliophora, Trichostomatia) Biyotası

Abstract

Bu çalışmada Kastamonu’daki Avrupa karacalarının (Capreolus capreolus) işkembe siliyat biyotası araştırılmıştır. Tek bir tür, Entodinium dubardi (Ophryoscolecidae, Trichostomatia) onyedi karacanın onikisinin (%70.6 yaygınlık) işkembe içeriğinden teşhis edilmiştir. Onyedi karacanın beşinde siliyat yoktur. E. dubardi Türkiye’deki karacaların (Capreolus capreolus) işkembe içeriğinden ilk defa tespit edilmiştir. Ortalama siliyat konsantrasyonu 17.5 ± 27.0 × 104 hücre mL–1’dir. Min.–maks. değerler 0’dan 87’ye × 104 hücre mL–1 değişmektedir.

Keywords

Cervidae, Entodinium dubardi, Ophryoscolecidae, mikroorganizma, ekosistem

References

• Argunov, A.V., & Stepanova, V.V. (2015). The winter diet of the Siberian roe deer (Capreolus pygargus Pall., 1771) in the Central Yakutia. Vestnik KrasGAU, 4, 138–143.
• Behrend, A., Lechner-Doll, M., Streich, W.J., & Clauss, M. (2004). Seasonal faecal excretion, gut fill, liquid and particle marker retention in mouflon Ovis ammon musimon and a comparison with roe deer Capreolus capreolus. Acta Theriologica, 49, 503–515. https://doi.org/10.1007/BF03192594
• Beşkardeş, V., Keten, A., & Arslangündoğdu, Z. (2008). The importance of roe deer (Capreolus capreolus L. 1758) in wildlife for Turkey. Forestist, 58, 15–22. https://doi.org/10.17099/jffiu.07636
• Brüggemann, J., Giesecke, D., & Walser-Käst, K. (1967). Beiträge zur Wildbiologie und vergleichenden Tierphysiologie. II. Mikroorganismen im Pansen von Rothirsch (Cervus elaphus) und Reh (Capreolus capreolus). Zeitschrift für Tierphysiologie Tierernährung und Futtermittelkunde, 23, 143–151. https://doi.org/10.1111/j.1439-0396.1967.tb00972.x
• Buisson, J. (1923). Sur quelques infusoires nouveaux ou peu connus parasites des mammifères. Annales de Parasitologie Humaine et Comparée, 1, 209–246. https://doi.org/10.1051/parasite/1923013209
• Clauss, M., Müller, K., Fickel, J., Streich, W.J., Hatt, J.-M., & Südekum, K.-H. (2011). Macroecology of the host determines microecology of endobionts: protozoal faunas vary with wild ruminant feding type and body mass. Journal of Zoology, 283, 169–185. https://doi.org/10.1111/j.1469-7998.2010.00759.x
• Cornelis, J., Casaer, J., & Hermy, M. (1999). Impact of season, habitat and research techniques on diet composition of roe deer (Capreolus capreolus): a review. Journal of Zoology, 248(2), 195–207. https://doi.org/10.1111/j.1469-7998.1999.tb01196.x
• Dehority, B.A. (1984). Evaluation of subsampling and fixation procedures used for counting rumen protozoa. Applied and Environmental Microbiology, 48(1), 182–185. https://doi.org/10.1128/aem.48.1.182-185.1984
• Dehority, B.A. (1986). Protozoa of the digestive tract of herbivorous mammals. Insect Science and Its Application, 7, 279–296. https://doi.org/10.1017/S1742758400009346
• Dehority, B.A. (1994). Rumen ciliate protozoa of the blue duiker (Cephalophus monticola), with observations on morphological variation lines within the species Entodinium dubardi. Journal of Eukaryotic Microbiology, 41(2), 103–111. https://doi.org/10.1111/j.1550-7408.1994.tb01481.x
• Dehority, B.A., & Odenyo, A.A. (2003). Influence of diet on the rumen protozoal fauna of indigenous African wild ruminants. The Journal of Eukaryotic Microbiology, 50(3), 220–223. https://doi.org/10.1111/j.1550-7408.2003.tb00121.x
• Dehority, B.A.& Orpin, C.G. (1997). Development of, and natural fluctuations in, rumen microbial populations. In: Hobson, P.N., & Stewart, C.S. (ed) The Rumen Microbial Ecosystem. Springer, Dordrecht, 196–245. https://doi.org/10.1007/978-94-009-1453-7_5
• Deutsch, A., Lechner-Doll, M., & Wolf, G.A. (1998). Activity of cellulolytic enzymes in the contents of reticulorumen and caecocolon of roe deer (Capreolus capreolus). Comparative Biochemistry and Physiology Part A: Molecular & Integrative Physiology, 119, 925–930. https://doi.org/10.1016/s1095-6433(98)00004-x
• Dogiel, V.A. (1927). Monographie der Familie Ophryoscolecidae. Archiv für Protistenkunde, 59, 1–288.
• Enzinger, W., & Hartfiel, W. (1998). Auswirkungen gesteigerter Energie- und Proteingehalte des Futters auf Fermentationsprodukte, Fauna und Schleimhaut des Pansens von Wildwiederkäuern (Damhirsch/Reh) im Vergleich zu Hauswiederkäuern (Schaf/Ziege). Zeitschrift für Jagdwissenschaft, 44, 201–220. https://doi.org/10.1007/BF02242026
• Evcin, Ö., Küçük, Ö., Akkuzu, E., & Ugış, A. (2017). Habitat preferences of roe deer (Capreolus capreolus) in Kastamonu: case study of Elekdağı wildlife development area. International Journal of Engineering Sciences & Research Technology, 6(4), 225–229. https://doi.org/10.5281/zenodo.546312
• Gürelli, G. (2016). Rumen ciliates of domestic cattle (Bos taurus taurus) in Kastamonu, Turkey with the description of a new species. European Journal of Protistology, 56, 51–59. https://doi.org/10.1016/j.ejop.2016.07.002
• Gürelli, G., & Daw A.F.O.E. (2020) Endosymbiotic ciliates protozoan biota of dromedary camels and domestic cattle in Tunisia. Zootaxa, 4859(3), 409–418. https://doi.org/10.11646/zootaxa.4859.3.6
• Hofmann, R.R. (1989). Evolutionary steps of ecophysiological adaptation and diversification of ruminants: a comparative view of their digestive system. Oecologia, 78, 443–457. https://doi.org/10.1007/BF00378733
• Hungate, R.E. (1966). The Rumen and Its Microbes. New York, Academic Press., 533 pp.
• Imai, S., Tsutsumi, Y., Yumura, S., & Mulenga, A. (1992). Ciliate protozoa in the rumen of Kafue lechwe, Kobus leche kafuensis, in Zambia, with the description of four new species. Journal of Protozoology, 39, 564–572. https://doi.org/10.1111/j.1550-7408.1992.tb04852.x
• Imai, S., Matsumoto, M., Watanabe, A., & Sato, H. (1993). Rumen ciliate protozoa in Japanese sika deer (Cervus nippon centralis). Animal Science and Technology, 64(6), 578–583.
• Kamler, J. (1999). Infusorial concentration in rumen fluid of red deer, fallow deer, roe deer an moufflon. Acta Veterinaria Brno, 68, 247–252. https://doi.org/10.2754/avb199968040247
• Keten, A. (2017). Distribution and habitat preference of roe deer (Capreolus capreolus L.) in Düzce Province (Turkey). Forestist, 67(1), 22–28. https://doi.org/10.17099/jffiu.89577
• Kornilova, O.A., (2004). History of Study of Endobiotic Ciliates of Mammalia. St-Petersburg, Tessa Press., 349 pp.
• Kornilova, O.A., Chistyakova, L.V., Seryodkin, I.V., & Grabarnik, I.P. (2021). Endobiotic ciliates from the rumen of the roe deer Capreolus pygargus. Parazitologiya, 55(6), 465–475. https://doi.org/10.31857/S0031184721060028
• Lynn, D.H. (2008). The Ciliated Protozoa, Characterization, Classification and Guide to the Literature. New York, Springer, 605 pp.
• Marinucci, M.T., Capecci, A., Riganelli, N., Acuti, G., Antonini, C., & Olivieri, O. (2005). Dietary preferences and ruminal protozoal populations in roe deer (Capreolus capreolus), fallow deer (Dama dama) and mouflon (Ovis musimon). Italian Journal of Animal Science, 4(2), 401–403. https://doi.org/10.4081/ijas.2005.2s.401
• Miltko, R., Kowalik, B., Majewska, M.P., Kedzierska, A, McEwan, N.R., & Belżecki, G. (2020). The effect of protozoa on the bacterial composition and hydrolytic activity of the roe der rumen. Animals, 10(467), 1–9. https://doi.org/10.3390/ani10030467
• Ogimoto, K., & Imai, S. (1981). Atlas of Rumen Microbiology. Tokyo, Japan Scientific Societies Press., 231 pp.
• Sládeček, F. (1946). Ophryoscolecidae from the stomach of Cervus elaphus L., Dama dama L., and Capreolus capreolus L. Vestnik Ceskoslovenske Spolecnosti Zoologicke, 10, 201–231.
• Wertheim, P. (1934). Über die beschaffenheit der infusorienmagenfauna von Capreolus capreolus L. Zoologischer Anzeiger, 106, 67–70.
• Williams, A.G., & Coleman, G.S. (1992). The Rumen Protozoa. New York, Springer, 442 pp. https://doi.org/10.1007/978-1-4612-2776-2
• Zielyk, M.W. (1961). Ophryoscolecid fauna from the stomach of the white-tailed deer (Odocoileus virginianus borealis), and observations on the division of Entodinium dubardi Buisson 1923 (Ciliata Entodiniomorpha). The Journal of Protozoology, 8(1), 33–41. https://doi.org/10.1111/j.1550-7408.1961.tb01178.x