COMPARISON OF TOXIC AND ANTITOXIC EFFECTS OF CISPLATIN AND CAMP, AT CYP1A1 GENE EXPRESSION LEVEL OF WISTAR ALBINO TYPE RAT LIVER

Year 2019, Volume: 28 Issue: 1, 91 - 100, 30.06.2019

Kumbirai Deon Mandebere F. Seyma Gokdemır , Gonul Solmaz , Sumer Aras

Abstract

Throughout the course of cancer treatment, cisplatin has proven to be a
successful chemotherapy agent. Several researches have been conducted in vivo and/or in
vitro on gene expression changes occurring with the impact of this agent. The current study
focuses on the alterations in CYP1A1 gene expression levels in liver cells by cisplatin and
cAMP treatment. CYP1A1 gene which the expression levels were analyzed, encodes a
member of the cytochrome P450 superfamily of enzymes. Throughout the study, liver
tissues from Wistar-type albino rat, also known as the laboratory rat, were used. For
normalization of the data, the housekeeping gene TATA box binding protein (TBP) was
preferred. The results showed that; gene expression was reduced in tissues subjected
independently to cisplatin and cAMP, however the tissues treated with cAMP showed
further reduction in gene expression levels. In contrast, tissues subjected to both cisplatin
and cAMP showed an increase in the level of expression. This study aims to provide an
alternative perspective for comparative toxicology cases and will also serve as a guide for
elucidation of cases connected with forensic toxicology.  

Keywords

cisplatin, cAMP, Wistar albino, cytochrome p450, gene expression, forensic toxicology

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