PUTRESCINE AS A PROTECTIVE MOLECULE ON DNA DAMAGE AND DNA METHYLATION CHANGES IN WHEAT UNDER DROUGHT

Year 2019, Volume: 28 Issue: 2, 170 - 187, 15.12.2019

Esra Arslan , Guleray Agar , Murat Aydın

Abstract

The world suffers with the agricultural drought stress which leading to
decreasing crop production, and also adversely affecting cereals on
morphological, physiological, biochemical and molecular levels. However,
exogenous treatment of some osmotically active materials like putrescine has
been regarded as a good preventive against these harmful effects of drought.
But there is a lack of information on putrescine has any effects on DNA damage
and DNA methylation in crops. The current study was goal to determine DNA
damage levels and DNA methylation changes in Triticum aestivum cv. Karasu 90 subjected to different
concentrations of drought (-2, -4, -6 bar PEG) and whether putrescine (0.01,
0.1, 1 mM) has any ameliorative effect on these changes is determined with
RAPDs and CRED-RAs tecniques. In addition, total oxidant status (TOS) and total
antioxidant status (TAS) values were investigated based on drought and
putrescine treatments. The findings showed that drought stress caused DNA
damage and DNA methylation changes. However, these effects decreased after
putrescine treatments. Putrescine has been shown to decrease oxidative damage
caused by drought via increasing antioxidant status in drought stress.
According to results, it was concluded that putrescine could be preferred for
its force to protect wheat DNA from the damaging effects of drought and the
demethylation positively contributed to drought stress tolerance.

Keywords

CRED-RA, DNA Methylation, Genomic Template Stability, RAPD, Total oxidant status, Total antioxidant status

Supporting Institution

Ataturk University

Project Number

2011/355

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