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BIOFILM FORMATION AND CONTROL OF FACULTATIVE THERMOPHILE BREVIBACILLUS AGRI D505B

Year 2020, Volume: 29 Issue: 1, 119 - 130, 30.06.2020

Abstract

Brevibacillus agri D505b is an aerobic, non-pathogenic, endospore-forming facultative thermophilic bacillus. Six abiotic surfaces (stainless steel, glass, polyvinyl chloride, polypropylene, polystyrene, and polycarbonate) were compared with viable cell enumerations. According to results, D505b cells could able to attach all these surfaces. Stainless steel (6.10 log CFU/cm2) was found to be the most effective surface for biofilm formation. Polycarbonate (6.03 log CFU/cm2) was found as the second best surface. Furthermore, the D505b biofilm was treated with 15 different sanitation agents and trichloroacetic acid (TCA) was determined to be the most effective one (80.3% removal). Our results showed that the strong biofilm producer B. agri D505b was very resistant to various sanitation agents. However, TCA significantly inhibited biofilm formation for the isolate. 

Supporting Institution

Ankara University

Project Number

11B4240003

References

  • Berlang, M., Guerrero, R., Living together in biofilms: the microbial cell factory and its biotechnological implications, Microbial Cell Factories, 15, (2016), 165.
  • Alvarez-Ordóñez, A, Coughlan, L.M., Briandet, R., Cotteri, P.D., Biofilms in food processing environments: Challenges and opportunities, Annual Review of Food Science and Technology, 10, (2019), 173-195.
  • Gupta, S., Anand, S., Induction of pitting corrosion on stainless steel (grades 304 and 316) used in dairy industry by biofilms of common sporeformers, International Journal of Dairy Technology, 71, (2018), 519-531.
  • Faille, C., Jullien, C., Fontaine, F., Bellon-Fontaine, M.N., Slomianny, C., Benezech, T., Adhesion of Bacillus spores and Escherichia coli cells to inert surfaces: role of surface hydrophobicity Canadian Journal of Microbiology, 48, (2002), 728-738.
  • Kolari, M., Nuutinen, J., Salkinoja-Salonen, M.S., Mechanisms of biofilm formation in paper machine by Bacillus species: the role of Deinococcus geothermalis, Journal of Industrial Microbiology and Biotechnology, 27, (2001), 343-351.
  • Gopal, N., Hill, C., Ross, P.R., Beresford, T.P., Fenelon, M.A., Cotter, P.D., The prevalence and control of Bacillus and related spore-forming bacteria in the dairy industry, Frontiers in Microbiology, 6, (2015), 1418.
  • Chebbi, A., Elshikh, M., Haque, F., Ahmed, S., Dobbin, S., Marchant, R., Sayadi, S., Chamkha, M., Banat, I.M., Rhamnolipids from Pseudomonas aeruginosa strain W10; as antibiofilm/antibiofouling products for metal protection, Journal of Basic Microbiology, 57, (2017), 364-375.
  • Parkar, S.G., Flint, S.H., Brooks, J.D., Physiology of biofilms of thermophilic bacilli—potential consequences for cleaning, Journal of Industrial Microbiology and Biotechnology, 30, (2003), 553-560.
  • Wirtanen, G., Husmark, U., Mattila-Sandholm, T., Microbial evaluation of the biotransfer potential from surfaces with Bacillus biofilms after rinsing and cleaning procedures in closed food-processing systems, Journal of Food Protection, 59, (1996), 727-733.
  • Parkar, S.G., Flint S.H., Brooks, J.D., Evaluation of the effect of cleaning regimes on biofilms of thermophilic bacilli on stainless steel, Journal of Applied Microbiology, 96, (2004), 110-116.
  • Burgess, S.A., Flint, S.H., Lindsay, D., Characterization of thermophilic bacilli from a milk powder processing plant, Journal of Applied Microbiology, 116, (2014), 350-359.
  • Tsiaprazi-Stamou, A., Monfort, I.Y., Romani, A.M., Bakalis, S., Gkatzionis, K., The synergistic effect of enzymatic detergents on biofilm cleaning from different surfaces, Biofouling, 35, (2019), 883-899.
  • Soltani, M.R.J., The lysis of micro-organisms by lysozyme and related enzymes, Microbiology, 18, (1958), 481-490.
  • Bierbaum, G., Sahl, H.G., Lantibiotics: mode of action, biosynthesis and bioengineering, Current Pharmaceutical Biotechnology, 10, (2009), 2-18.
  • And, H.C., Hoover, D.G., Bacteriocins and their food applications, Comprehensive Reviews in Food Science and Food Safety, 2, (2003), 82-100.
  • Takao, A., Nakano, M., Ikeno, M., Ozawa, T., Hosoya, N., Maeda, N., Effect of sodiumpercarbonate-containing disinfectant on natural biofilm model of dental unit waterline, Asian Pacific Journal of Dentistry, 16, (2016), 29-34.
  • Cihan, A.C., Karaca, B., Ozel, P.B., Kilic, T., Determination of the biofilm production capacities and characteristics of members belonging to Bacillaceae family, World Journal of Microbiology and Biotechnology, 33, (2017), 118.
  • Cihan, A.C., Tekin, N., Ozcan, B., Cokmus, C., The genetic diversity of genus Bacillus and the related genera revealed by 16S rRNA gene sequences and ardra analyses isolated from geothermal regions of Turkey, Brazilian Journal of Microbiology, 43, (2012), 309-324.
  • Giaouris, E.D., Nychas, G.J.E., The adherence of Salmonella Enteritidis PT to stainless steel: The importance of the air–liquid interface and nutrient availability, Food Microbiology, 23, (2006), 747-752.
  • Herigstad, B., Hamilton, M., Heersink, J., How to optimize the drop plate method for enumerating bacteria, Journal of Microbiological Methods, 44, (2001), 121-129.
  • Pitts, B., Hamilton, M.A., Zelver, N., Stewart, P.S., A microtiter-plate screening method for biofilm disinfection and removal, Journal of Microbiological Methods, 54, (2003), 269-276.
  • Ponnusamy, K., Paul, D., Kim, Y.S., Kweon, J.H., 2 (5H)-Furanone: a prospective strategy for biofouling-control in membrane biofilm bacteria by quorum sensing inhibition, Brazilian Journal of Microbiology, 41, (2010), 227-234.
  • Tabak, M., Scher, K., Hartog, E., Romling, U., Matthews, K.R., Chikindas, M.L,, Yaron, S., Effect of triclosan on Salmonella typhimurium at different growth stages and in biofilms, FEMS Microbiology Letters, 267, (2007), 200-206.
  • Rönner, U., Husmark, U., Henriksson, A., Adhesion of Bacillus spores in relation to hydrophobicity, Journal of Applied Bacteriology, 69, (1990), 550-556.
  • Elhariry, H.M., Biofilm formation by endospore-forming bacilli on plastic surface under some food-related and environmental stress conditions, Global Journal of Biotechnology and Biochemistry, 3, (2008), 69-78.
  • Stepanović, S., Ćirković, I., Ranin, L., Svabić‐Vlahović, M., Biofilm formation by Salmonella spp. and Listeria monocytogenes on plastic surface, Letters in Applied Microbiology, 38, (2004), 428-432.
  • Tuson, H.H., Weibel, D.B., Bacteria–surface interactions. Soft Matter, 9, (2013), 4368-4380.
  • Brooks, J.D., Flint, S.H., Biofilms in the food industry: problems and potential solutions, International Journal of Food Science and Technology, 43, (2008), 2163-2176.
  • Mafu, A.A., Roy, D., Goulet, J., Magny, P., Attachment of Listeria monocytogenes to stainless steel, glass, polypropylene, and rubber surfaces after short contact times, Journal of Food Protection, 53, (1990), 742-746.
  • Song, L., Wu, J., Xi, C., Biofilms on environmental surfaces: evaluation of the disinfection efficacy of a novel steam vapor system, American Journal of Infection Control, 40, (2012), 926-930.
  • Lücking, G., Stoeckel, M., Atamer, Z., Hinrichs, J., Ehling-Schulz, M., Characterization of aerobic spore-forming bacteria associated with industrial dairy processing environments and product spoilage, International Journal of Food Microbiology, 166, (2013), 270-279.
  • Frank, J.F., Koffi, R.A., Surface-adherent growth of Listeria monocytogenes is associated with increased resistance to surfactant sanitizers and heat, Journal of Food Protection, 53 (1990), 550-554.
  • Eladawy, M., El-Mowafy, M., El-Sokkary, M.M.A., Barwa, R., Effects of lysozyme, proteinase K, and cephalosporins on biofilm formation by clinical isolates of Pseudomonas aeruginosa, Interdisciplinary Perspectives on Infectious Diseases, 2020 (2020).
  • Panda, A.K., Bisht, S.S., DeMondal, S., Kumar, N.S., Gurusubramanian, G., Panigrahi, A.K., Brevibacillus as a biological tool: a short review, Antonie van Leeuwenhoek, 105 (2014), 623-639.
  • Burgess, S.A., Lindsay, D., Flint, S.H., Thermophilic bacilli and their importance in dairy processing, International Journal of Food Microbiology, 144 (2010), 215-225.
Year 2020, Volume: 29 Issue: 1, 119 - 130, 30.06.2020

Abstract

Project Number

11B4240003

References

  • Berlang, M., Guerrero, R., Living together in biofilms: the microbial cell factory and its biotechnological implications, Microbial Cell Factories, 15, (2016), 165.
  • Alvarez-Ordóñez, A, Coughlan, L.M., Briandet, R., Cotteri, P.D., Biofilms in food processing environments: Challenges and opportunities, Annual Review of Food Science and Technology, 10, (2019), 173-195.
  • Gupta, S., Anand, S., Induction of pitting corrosion on stainless steel (grades 304 and 316) used in dairy industry by biofilms of common sporeformers, International Journal of Dairy Technology, 71, (2018), 519-531.
  • Faille, C., Jullien, C., Fontaine, F., Bellon-Fontaine, M.N., Slomianny, C., Benezech, T., Adhesion of Bacillus spores and Escherichia coli cells to inert surfaces: role of surface hydrophobicity Canadian Journal of Microbiology, 48, (2002), 728-738.
  • Kolari, M., Nuutinen, J., Salkinoja-Salonen, M.S., Mechanisms of biofilm formation in paper machine by Bacillus species: the role of Deinococcus geothermalis, Journal of Industrial Microbiology and Biotechnology, 27, (2001), 343-351.
  • Gopal, N., Hill, C., Ross, P.R., Beresford, T.P., Fenelon, M.A., Cotter, P.D., The prevalence and control of Bacillus and related spore-forming bacteria in the dairy industry, Frontiers in Microbiology, 6, (2015), 1418.
  • Chebbi, A., Elshikh, M., Haque, F., Ahmed, S., Dobbin, S., Marchant, R., Sayadi, S., Chamkha, M., Banat, I.M., Rhamnolipids from Pseudomonas aeruginosa strain W10; as antibiofilm/antibiofouling products for metal protection, Journal of Basic Microbiology, 57, (2017), 364-375.
  • Parkar, S.G., Flint, S.H., Brooks, J.D., Physiology of biofilms of thermophilic bacilli—potential consequences for cleaning, Journal of Industrial Microbiology and Biotechnology, 30, (2003), 553-560.
  • Wirtanen, G., Husmark, U., Mattila-Sandholm, T., Microbial evaluation of the biotransfer potential from surfaces with Bacillus biofilms after rinsing and cleaning procedures in closed food-processing systems, Journal of Food Protection, 59, (1996), 727-733.
  • Parkar, S.G., Flint S.H., Brooks, J.D., Evaluation of the effect of cleaning regimes on biofilms of thermophilic bacilli on stainless steel, Journal of Applied Microbiology, 96, (2004), 110-116.
  • Burgess, S.A., Flint, S.H., Lindsay, D., Characterization of thermophilic bacilli from a milk powder processing plant, Journal of Applied Microbiology, 116, (2014), 350-359.
  • Tsiaprazi-Stamou, A., Monfort, I.Y., Romani, A.M., Bakalis, S., Gkatzionis, K., The synergistic effect of enzymatic detergents on biofilm cleaning from different surfaces, Biofouling, 35, (2019), 883-899.
  • Soltani, M.R.J., The lysis of micro-organisms by lysozyme and related enzymes, Microbiology, 18, (1958), 481-490.
  • Bierbaum, G., Sahl, H.G., Lantibiotics: mode of action, biosynthesis and bioengineering, Current Pharmaceutical Biotechnology, 10, (2009), 2-18.
  • And, H.C., Hoover, D.G., Bacteriocins and their food applications, Comprehensive Reviews in Food Science and Food Safety, 2, (2003), 82-100.
  • Takao, A., Nakano, M., Ikeno, M., Ozawa, T., Hosoya, N., Maeda, N., Effect of sodiumpercarbonate-containing disinfectant on natural biofilm model of dental unit waterline, Asian Pacific Journal of Dentistry, 16, (2016), 29-34.
  • Cihan, A.C., Karaca, B., Ozel, P.B., Kilic, T., Determination of the biofilm production capacities and characteristics of members belonging to Bacillaceae family, World Journal of Microbiology and Biotechnology, 33, (2017), 118.
  • Cihan, A.C., Tekin, N., Ozcan, B., Cokmus, C., The genetic diversity of genus Bacillus and the related genera revealed by 16S rRNA gene sequences and ardra analyses isolated from geothermal regions of Turkey, Brazilian Journal of Microbiology, 43, (2012), 309-324.
  • Giaouris, E.D., Nychas, G.J.E., The adherence of Salmonella Enteritidis PT to stainless steel: The importance of the air–liquid interface and nutrient availability, Food Microbiology, 23, (2006), 747-752.
  • Herigstad, B., Hamilton, M., Heersink, J., How to optimize the drop plate method for enumerating bacteria, Journal of Microbiological Methods, 44, (2001), 121-129.
  • Pitts, B., Hamilton, M.A., Zelver, N., Stewart, P.S., A microtiter-plate screening method for biofilm disinfection and removal, Journal of Microbiological Methods, 54, (2003), 269-276.
  • Ponnusamy, K., Paul, D., Kim, Y.S., Kweon, J.H., 2 (5H)-Furanone: a prospective strategy for biofouling-control in membrane biofilm bacteria by quorum sensing inhibition, Brazilian Journal of Microbiology, 41, (2010), 227-234.
  • Tabak, M., Scher, K., Hartog, E., Romling, U., Matthews, K.R., Chikindas, M.L,, Yaron, S., Effect of triclosan on Salmonella typhimurium at different growth stages and in biofilms, FEMS Microbiology Letters, 267, (2007), 200-206.
  • Rönner, U., Husmark, U., Henriksson, A., Adhesion of Bacillus spores in relation to hydrophobicity, Journal of Applied Bacteriology, 69, (1990), 550-556.
  • Elhariry, H.M., Biofilm formation by endospore-forming bacilli on plastic surface under some food-related and environmental stress conditions, Global Journal of Biotechnology and Biochemistry, 3, (2008), 69-78.
  • Stepanović, S., Ćirković, I., Ranin, L., Svabić‐Vlahović, M., Biofilm formation by Salmonella spp. and Listeria monocytogenes on plastic surface, Letters in Applied Microbiology, 38, (2004), 428-432.
  • Tuson, H.H., Weibel, D.B., Bacteria–surface interactions. Soft Matter, 9, (2013), 4368-4380.
  • Brooks, J.D., Flint, S.H., Biofilms in the food industry: problems and potential solutions, International Journal of Food Science and Technology, 43, (2008), 2163-2176.
  • Mafu, A.A., Roy, D., Goulet, J., Magny, P., Attachment of Listeria monocytogenes to stainless steel, glass, polypropylene, and rubber surfaces after short contact times, Journal of Food Protection, 53, (1990), 742-746.
  • Song, L., Wu, J., Xi, C., Biofilms on environmental surfaces: evaluation of the disinfection efficacy of a novel steam vapor system, American Journal of Infection Control, 40, (2012), 926-930.
  • Lücking, G., Stoeckel, M., Atamer, Z., Hinrichs, J., Ehling-Schulz, M., Characterization of aerobic spore-forming bacteria associated with industrial dairy processing environments and product spoilage, International Journal of Food Microbiology, 166, (2013), 270-279.
  • Frank, J.F., Koffi, R.A., Surface-adherent growth of Listeria monocytogenes is associated with increased resistance to surfactant sanitizers and heat, Journal of Food Protection, 53 (1990), 550-554.
  • Eladawy, M., El-Mowafy, M., El-Sokkary, M.M.A., Barwa, R., Effects of lysozyme, proteinase K, and cephalosporins on biofilm formation by clinical isolates of Pseudomonas aeruginosa, Interdisciplinary Perspectives on Infectious Diseases, 2020 (2020).
  • Panda, A.K., Bisht, S.S., DeMondal, S., Kumar, N.S., Gurusubramanian, G., Panigrahi, A.K., Brevibacillus as a biological tool: a short review, Antonie van Leeuwenhoek, 105 (2014), 623-639.
  • Burgess, S.A., Lindsay, D., Flint, S.H., Thermophilic bacilli and their importance in dairy processing, International Journal of Food Microbiology, 144 (2010), 215-225.
There are 35 citations in total.

Details

Primary Language English
Subjects Structural Biology
Journal Section Research Articles
Authors

Tuğba Kılıç 0000-0002-5474-0288

Arzu Çöleri Cihan 0000-0002-5474-0288

Project Number 11B4240003
Publication Date June 30, 2020
Acceptance Date March 27, 2020
Published in Issue Year 2020 Volume: 29 Issue: 1

Cite

Communications Faculty of Sciences University of Ankara Series C-Biology.

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This work is licensed under a Creative Commons Attribution 4.0 International License.