EFFECTS OF β-ESTRADIOL ON DNA METHYLATION CHANGES AND GENOMIC STABILITY IN TRITICUM AESTIVUM L. EXPOSED SALT

Year 2020, Volume: 29 Issue: 1, 161 - 175, 30.06.2020

Murat Aydın , Semra Yağci , Güleray Ağar , Mahmut Sinan Taşpınar

Abstract

Salinity stress is considered one of the most important agricultural problems because it causes yield loss. Although it is well known that salinity damages to DNA and results in DNA methylation changes in plants, there is no report investigating the effect of mammalian hormones in plants growing under salinity stress. Therefore, the present study wasa imed at investigating DNA damage levels, DNA stability and DNA methylation changes in Triticum aestivum subjected to salinity stress and determine whether β-estradiol has any effect on these changes. RAPD (Randomly Amplified Polymorphic DNA) and CRED-RA (Coupled Restriction Enzyme Digestion-Random Amplification) were used to define the DNA damage levels and changes in the pattern of DNA methylation. The results showed that salinity stress led to an increase in RAPDs profile changes (DNA damage), a reduction in genomic template stability (GTS) and DNA hypermethylation. The effects caused by salinity stress were decreased after treatment with different concentrations of β-estradiol. The results of this study clearly show that β-estradiol could be used effectively to protect wheat seedlings from the destructive effects of salinity stress, and demethylation positively contributed to salinity stress tolerance.

Keywords

β-estradiol, DNA damage, DNA methylation

Supporting Institution

no

Project Number

no

References

• Erdal, S., Aydın, M., Genisel, M., Taspinar, M.S., Dumlupinar, R., Kaya, O., Gorcek, Z., Effects of salicylic acid on wheat salt sensitivity, African Journal of Biotechnology, 10 (2011), 5713-5718.
• Erdal, S., Alleviation of salt stress in wheat seedlings by mammalian sex hormones, Journal of the Science of Food and Agriculture, 92 (2011) , 1411-1416.
• Paridaa, A.K., Das, A.B., Salt tolerance and salinity effects on plants, Ecotoxicology Environental Safety, 60 (2005), 324-349.
• Gorai, M., Ennajeh, M., Khemira, H, Neffati, M., Influence of NaCl-salinity on growth, photosynthesis, water relations and solute accumulation in Phragmites australis, Acta Physiologiae Plantarum, 33 (2011), 963-971.
• Cho, D., Shin, D, Jeon, W.B., Kwak, J.M., ROS-mediated ABA signaling, Journal of Plant Biology, 52 (2009), 102-113.
• Si, Y., Zhang, C., Meng, S., Dane, F., Gene expression changes in response to drought stres in citrullus colocynthis, Plant Cell Reports, 28 (2009), 997-1009.
• Tan, M.P., Analysis of DNA methylation of maize in response to osmotic and salt stress based on methylation-sensitive amplified polymorphism, Plant Physiology and Biochemistry, 48 (2010), 21-26.
• Li, X.L., Lin, Z.X., Nie, Y.C., Guo, X.P., Zhang, X.L., Methylation sensitive amplification polymorphism of epigenetics changes in cotton under salt stres, Acta Agronomica Sinica, 35 (2009), 588–596.
• Zhao, Y., Yu, S., Ye, W., Wang, H., Wang, J., Fang, B., Study on DNA cytosine methylation of cotton (Gossypium hirsutum L.) genome and its implication for salt tolerance, Agriculturel Science in China, 9 (2010), 783-791.
• Paszkowski, J., Whitham, S.A., Gene silencing and DNA methylation processes, Current Opinion in Plant Biology, 4 (2001), 123-129.
• Tran, R.K., Henikoff, J., Zilberman, D., Ditt, R., Jacobsen, S., Benioff, S., DNA methylation profiling identifies CpG methylation clusters in Arabidopsis genes, Current Opinion in Plant Biology, 15 (2005), 154-159.
• Zhou, J., Wang, X., Jiao, Y., Qin, Y., Liu, X., He, K., Chen, C., Ma, L., Wang, J., Xiong, L., Zhang, Q., Fan, L., Deng, X.W., Global genome expression analysis of rice in response to drought and high-salinity in shoot, flag leaf, and panicle, Plant Molecular Biology, 63 (2007), 591–608.
• Cao, S., Xu, Q., Cao, Y., Qian, K., An, K., Zhu, Y., Loss-of-function mutations in DET2 gene lead to an enhanced resistance to oxidative stress in Arabidopsis, Plant Physiology, 123 (2005), 57–66.
• Xiong, L.M., Schumaker, K.S., Zhu, J.K., Cell signaling during cold, drought, and salt stres, Plant Cell, 14 (2002), 165–183.
• Erdal, S., Dumlupinar, R., Xogenously treated mammalian sex hormones affects inorganic constituents of plants, Biological Trace Element Research, 143 (2011), 500–506.
• Dogra, R. Thukral, A.K., Effect of steroid on some inorganic constituents of wheat plants, Current Research Plant Science, 2 (1996), 155–160.
• Erdal, S., Dumlupinar, R., Progesterone and β-estradiol stimulate the seed germination in chickpea by causing important changes in biochemical parameters, Zeitschrift für Naturforschung C, 65 (2010), 239-244.
• Erdal, S., Dumlupinar, R., Cakmak, T., Taskin, M., Determination of some inorganic element concentration changes in germinating chickpea seeds exposed to progesterone and β-estradiol by using WDXRF spectroscopic technique, Fresenius Environmental Bulletin, 19 (2010), 507–515.
• Arslan, E., Ağar, G., Aydın, M., Putrescine as a protective molecule on DNA damage and DNA methylation changes in wheat under drought, Communications Faculty of Science University Ankara Series C, 28 (2019), 170-187.
• Jang, S. Jin, Wi, S.J., Choi, Y.J., An1, G., Park, K.Y., Increased Polyamine Biosynthesis Enhances Stress Tolerance by Preventing the Accumulation of Reactive Oxygen Species: T-DNA Mutational Analysis of Oryza sativa Lysine Decarboxylase-like Protein 1, Molecular Cells, 34 (2012), 251-262.
• Lu, G., Wu, X., Chen, B., Gao, G., Xu, K., Evaluation of genetic and epigenetic modification in Rapeseed (Brassica napus) induced by salt stress, Journal of Integrative Plant Biology, 49 (2007), 1599-1607.
• Ge, C.L., Yang, X.Y., Liu, X.N., Sun, J.H., Luo, S.S., Wang, Z.G., Effect of heavy metal on levels of methylation in DNA of rice and wheat, Journal of Plant Physiology and Molecular Biology, 28 (2002), 363-368.
• Liu, W., Yang, Y.S., Zhou, Q., Xie, L., Li, P., Sun, T., Impact assessment of cadmium contamination on rice (Oryza sativa L.) seedlings at molecular and population levels using multiple biomarkers, Chemosphere, 67 (2007), 1155-1163.
• Taspinar, M.S., Agar, G., Yildirim, N., Sunar, S., Aksakal, O., Bozari, S., Evaluation of selenium effect on cadmium genotoxicity in Vicia faba using RAPD, Journal of Food, Agriculture & Environment, 7 (2009), 857-860.
• Yıldız, M., Ciğerci, I., Bozdag, A., Terzi, H., Terzi, ESA., Evaluation of 2,4-D and dicamba genotoxicity in bean seedlings using comet and RAPD assays, Ecotoxicology and Environmental Safety, 73 (2010a), 1558-1563.
• Cenkci, S., Cigerci, I.H., Yildiz, M., Ozay, C., Bozdag, A., Terzi, H., Lead contamination reduces chlorophyll biosynthesis and genomic template stability in Brassica rapa L. Environmental and Experimental Botany, 67 (2010b), 467-473.
• Bano, A., Ullah, F., Nosheen, A., Role of abscisic acid and drought stress on the activities of antioxidant enzymes in wheat, Plant Soil Environment, 58 (2012), 181-185.
• Suzuki, N., Koussevitzky, S., Mittler, R., Miller, G., ROS and redox signalling in the response of plants to abiotic stress, Plant, Cell and Environment, 35 (2012), 259-270.
• Habu, Y., Kakutani, T., Paszkowski, J., Epigenetic developmental mechanisms in plants: molecules and targets of plant epigenetic regulation, Current Opinion In Genetics and Development, 11 (2001), 215-220.
• Wu, T., Pi, E.X., Tsai, S.N., Lam, H.M., Sun, S.M., Kwan, Y.W., Ngai, S.M., GmPHD5 acts as an important regulator for crosstalk between histone H3K4 di-methylation and H3K14 acetylation in response to salinity stress in soybean, Plant Biology, (2011), 11-178.
• Kovarik, A., Koukalova, B., Bezdek, M., Opatrny, Z., Hypermethylation of tobacco heterochromatic loci in response to osmotic stress, Theoretical and Applied Genetics, 95 (1997), 301-306.
• Kumar, S., Kaur, G., Nayyar, H., Exogenous application of abscisic acid improves cold tolerance in chickpea (Cicer arietinum L.). Journal Agronomy and Crop Science, 194 (2008), 449-456.
• Zhong, L., Xu, Y.H., Wang, J.B., The effect of 5-azacytidine on wheat seedlings responses to NaCl stress, Biologia Plantarum, 54 (2010), 753-756.
• Zhao, Y., Yu, S., Ye, W., Wang, H., Wang, J., Fang, B., Study on DNA Cytosine Methylation of Cotton (Gossypium hirsutum L.) Genome and Its Implication for Salt Tolerance, Agricultural Sciences in China, 9 (2010), 783-791.
• Erdal, S., Dumlupinar, R., Mammalian sex hormones stimulate antioxidant system and enhance growth of chickpea plants, Acta Physiololgy, Plant 33 (2011), 1011-1017.
• Erdal, S., Dumlupinar, R., Exogenously treated mammalian sex hormones affects inorganic constituents of plants, Biological Trace Element Research, 143 (2011), 500-506.
• Erdal, S., Dumlupinar, R., Cakmak, T., Genisel, M., Mammalian sex hormones influence germination velocity and enzyme activities in germinating maize seeds, Fresenius Environental Bulletin, 19 (2010), 1458-1465.
• Dogra, R., Thukral, A.K., Effect of steroid on some inorganic constituents of wheat plants, Journal of Plant Science: Current Research, 2 (1996), 155-160.
• Afzal, I., Basara, S.M.A., Nawaz, A. Faooq Mand, Alleviation of salinity stress in spring wheat by hormonal priming with ABA, salicylic acid and ascorbic acid, International Journal of Agriculture and Biology, 8 (2006), 23-28.
• Karl, H., Lauchli, M.A., Interaction of NaCl and Cd stress on compartmentation pattern of cations, antioxidant enzymes and proteins in leaves of two wheat genotypes differing in salt tolerance, Biology Plant., 43 (2000), 245-251.