HIGHLIGHTING THE MELISSOPALYNOLOGICAL AND PHYSICOCHEMICAL CHARACTERISTICS OF AYDER-RİZE (TURKEY)

Year 2021, Volume: 30 Issue: 2, 119 - 133, 31.12.2021

Omur Gencay Çelemli , Aslı Özkök , Çiğdem Özenirler , Nazlı Mayda , Golshan Zare , Kadriye Sorkun

https://doi.org/10.53447/communc.906788

Cited By: 2

Abstract

The aim of this research is characterizing the honey produced in Ayder/Rize-Turkey. In this context 20 honey samples were collected from Ayder in 2018. The melissopalynological and physicochemical analysis of the honey samples were done by this research. According to the melissopalynological analysis 40 taxa belong to the 22 plant families were identified as botanical sources of the investigated honey samples. Nine of the investigated samples (sample no 1-9) were evaluated as monofloral and the others as multifloral honeys. As a result of melissopalynological analysis, while pollen belong to the Castanea sativa taxon were found in dominant ratios in some of the investigated samples, the pollen belongs to the Castanea sativa, Trifolium repens, Lotus corniculatus, Coronilla orientalis taxa were found as secondary in some other honey samples. Total pollen number in 10 gram honey (TPN 10) values of the samples were found between 7 732 and 167 147 by melissopalynological analysis. The first step of the physicochemical analsyis was the moisture analysis and the values found between 15,8% and 18,8% (mean value:7,01±0,98 %). The fructose/glucose analysis were done by High Performance Liquid Chromatography (HPLC) and the values for fructose found as: 26,43-35,57g/100g (mean: 31,39±2,41 g/100g), glucose values as; 20,11-30,58 g/100g (mean: 26,83±2,16 g/100g). Also fructose/glucose ratio was found as 1,03-1,34 (mean: 1,17±0,10). Hydroxymethylfurfural (HMF) and total phenolic acids analysis were done also by HPLC. The results for HMF analysis is between 0,7- 11,31 ppm (mean: 3,8±2,6 ppm ), for total phenolic acids the value are found between 88,92±0,04 mgGAE/kg - 196,17±0,10 mgGAE/kg (mean: 121,98±0,1 mgGAE/kg). As a step of chemical analysis; the volatile compounds of the honey samples were determined by Gas Chromatography and Mass Spectrometry (GC-MS). The compounds belong to the aldehydes, aliphatic acids and esters, alcohols, hydrocarbons, carboxylic acids and their esters, ketones, terpenes, fatty acids and their esters groups were found as a result of GC-MS analysis. The compounds belong to the carboxylic acids and their esters, fatty acids and their esters identified in higher ratios compare to the other compounds. Since, there is little detailed published information about the quality and properties of Ayder-Rize honey, the current study aims to characterize the honey belong to the this region.

Keywords

HONEY, melissopalynology, TPN10, HMF, GC-MS, total phenolic

References

• Liyanage, D.A.M., Arawwawala, Horadugoda G.S.P., Hewageegana, Health benefits and traditional uses of honey: A review, Journal of Apitherapy, 2 (2017) 1, 9-14. https://doi.org/10.5455/ja.20170208043727
• Anklam, E., A review of the analytical methods to determine the geographical and botanical origin of honey. Food Cemistry, 63 (4) (1998) 549-562. https://doi.org/10.1016/S0308-8146(98)00057-0
• Sanz, M.L., Gonzalez, M., Lorenzo, C., Sanz, J., Castro, I., A contribution to the differentation between nectar honey and honeydew honey, Food Chemistry, 91 (2005) 313-317.
• Abeshu, M.A., Geleta, B., Medicinal Uses of Honey. Biol Med (Aligarh) 8 (2016) 279. https://doi.org/10.4172/0974-8369.1000279.
• Qustani, M.A., Das Mikroskopishe Bild der Honige des östlichen Mittelmeergebites, Dissertation zur Erlangung des Grades eines Doktors der Naturwissenhaften vorgelegt dem Fachbereich allegemeine Naturwissenhaften der univesität Hohenheim (1976).
• Sorkun, K., Yuluğ, N., Erzurum Yöresi Ballarının Polen Analizi ve Antimikrobik Özellikleri. 21. Türk Mikrobiyoloji Kongresi, Girne, (1984) 93-100.
• Gür, N., Elazığ İlinde arıcılığın yoğun olduğu yörelerin ballarında polen analizi. Yüksek lisans tezi. Fırat Üni. Fen bilimleri enstitüsü. (1993) 29syf.
• Kaplan, A., Konya yöresi ballarında polen analizi. Yüksek lisans tezi, Ankara Üni. Fen Bilimleri Enstitüsü, (1993) 69 syf.
• Türker, M., Gümüşhane Ballarında polen analizi. Yüksek lisans tezi, Yüzüncü Yıl Üni. Fen Bilimleri Enstitüsü. Van, (1993) 35 syf.
• Sorkun, K., Doğan, C., Türkiye’nin Çeşitli yörelerinden toplanan ballarında polen analizi Hacettepe Fen ve Mühendislik Bilim Dergisi, 16 (1995) 15-24.
• Silici, S., Antioxidant and physicochemical properties of chesnut honeys from Turkey, Communication Faculty of Sciences University of Ankara Series C Biology, 2 (2018) 104-114. https://doi.org/10.1501/commuc_0000000204
• Can, Z., Yıldız, O., Şahin, H., Turumtay, E.A., Silici, S., Kolaylı, S., An investigation of Turkish honeys: Their physicochemical properties, antioxidant capacities and phenolic profiles, Food Chemistry, 180 (2015), 133-141. https://doi.org/10.1016/j.foodchem.2015.02.024
• Kıvrak, Ş., Kıvrak, İ., Karababa, E., Characterization of Turkish honeys regarding of physicochemical properties, and their adulteration analysis, Food Science and Technology, Food Sci. Technol, Campinas, 37 (1) (2017) 80-89. https://doi.org/10.1590/1678-457X.07916
• Derebaşı E, Bulut G., Col M., Güney F.,Yaşar N., Ertürk Ö., Physicochemical and residue analysis of honey from Black Sea Region of Turkey, Fresenius Environmental Bulletin, 23 (1) (2014), 10-17.
• Malkoç, M., Çakır, H., Kara, Y., Can, Z., Kolaylı, S., Phenolic composition and antioxidant properties of Anzer honey from Black Sea Region of Turkey, Uludağ Bee Journal, 19 (2019) 143-151. https://doi.org/10.31467/uluaricilik.602906
• Kanbur, E.D., Yüksek, T., Atamov, V., Özçelik, A.E., A comparison of the physicochemical properties of chesnut and highland honey: the case of Senoz Valley in the Rize Province of Turkey, Food Chemistry, 345 (2021). https://doi.org/10.1016/j.foodchem.2020.128864
• Kolaylı, S., Aliyazıcıoğlu, R., Ulusoy, E., Karaoğlu, Ş., Antioxidant and Antimicrobial Activities of Selected Turkish Honeys, Hacettepe J. Biol. & Chem., 36 (2), (2008) 163-172.
• Louveaux, J., Maurizio, A., Vorwohl, G., Methods of melissopalynology. Bee World. 59, (1978) 139-153. https://doi.org/10.1080/0005772X.1978.11097714
• Moar N.T., Pollen analysis of New Zealand Honey. Journal of Agricultural Research, (1985) 28-38. https://doi.org/10.1080/0005772X.1978.11097714
• Maurizio, A., Microscopy of honey. In: Crane, E. (Ed.), Honey: a Comprehensive Survey. Heinemann in cooperation with the Int. Bee Res. Ass, London, (1975) 240-257.
• IHC, (2009) International Honey Commission-Harmonised Methods of the International Honey Commission.
• Slinkard, K., Singleton, V.L., Total phenol analyses: automation and comparison with manual methods. AJEV. 28 (1) (1977) 49–55.
• Codex Alimentarius Commission. (2001). Revised Codex Standard for honey, Codex STAN 12–1981, Rev. 1 (1987), Rev., 2.
• Turkish Food Codex, Honey Directive (2020).
• Demir, E., Ayder-Ceymakçur (Çamlıhemşin-Rize) yaylalarının florası ve yöre ballarının kimyasal ve palinolojik özellikleri, Yüksek Lisans Tezi, Recep Tayyip Erdoğan Üniversitesi, (2013) 130syf.
• Hepsağ, F., Rize’deki Anzer Yaylası’nın Endemik Çiçeklerinden Üretilen Anzer Balının Toplam Fenolik Bileşiklerinin ve antioksidan Kapasitesinin Belirlenmesi, GIDA, 44(4) (2019) 641-653. https://doi.org/10.15237/gida.GD19046
• Çakır, Y., Çobanoğlu, D., Dervişoğlu, G., Koçyiğit, S., Karahan, D., Yelkovan, S., Determination of Antimicrobial Activity, Palynological Characteristics and Chemical Composition of Some Honey Samples from Turkey, Mellifera, 20(1) (2020), 41-60.