Effects of oral antidiabetic metformin on hepatocellular cancer cells

Year 2018, Volume: 43 Issue: 3, 557 - 561, 30.09.2018

Ayla Solmaz Avcıkurt , Eren Altun

https://doi.org/10.17826/cumj.341138

Abstract

Purpose: In this study, the effects of the oral antidiabetic metformin on cell proliferation on human hepatocellular carcinoma cell line (Hep3B) at 4 different doses and at 4 different time intervals were determined.

Materials and Methods: Hep3B cell line was used in this study. The cytotoxic effects of metformin on the Hep3B cells at 4 different doses (0.5, 1, 5 10 mM) and at 4 different time intervals (3, 6, 24 and 48 hours) were determined. The results were statistically analyzed. The dose and time specific effects of metformin on cell proliferation was determined.

Results: While metformin did not affect the cell proliferation of Hep3B cells at 3, 6 and 48 h groups according to the control group, the rate of cell proliferation was decreased at 5 mM and 10 mM doses and at 24 h.

Conclusion: Metformin was administered on Hep3B cells at different time intervals and at different doses and its effect on cell viability was determined by MTT assay. The cell proliferation and viability of the Hep3B cells were significantly dereased at 5 mM and 10 mM doses and at 24 h. No effect was observed on cell viability at other time intervals and at other doses.

Keywords

Liver Cancer, Metformin, Hep3B, Cytotoxicity

Oral antidiyabetik metforminin hepatosellüler kanser hücreleri üzerine etkisi

Abstract

Amaç: Bu çalışmada oral antidiyabetik metforminin 4 farklı dozda ve 4 farklı saat aralığında insan hepatosellüler kanser hücre hattında (Hep3B) hücre canlılığı üzerine etkisi araştırılmıştır.

Gereç ve Yöntem: Çalışmada Hep3B hücre hattı kullanılmıştır. Metforminin farklı dozlarda ve farklı zaman aralıklarında oluşturduğu sitotoksik etkinin belirlenmesi için 0.5, 1, 5 ve 10 mM dozunda metformine 3, 6, 24 ve 48 saat süreyle maruz kalan Hep3B hücrelerine MTT testi uygulanmıştır. Sonuçlar istatistiksel olarak analiz edilerek yorumlanmıştır. Metforminin doza ve zamana spesifik hücre proliferasyonu üzerine etkisi belirlenmiştir.

Bulgular: Metforminin Hep3B hücre hattında hücre proliferasyonu üzerine 3., 6. ve 48. saatlerde kontrol grubuna göre herhangi bir etkisi görülmezken, 24. saatte 5 ve 10mM dozlarında hücre proliferasyonunda azalış gözlenmiştir. 

Sonuç: Metformin Hep3B hücre hattına farklı zaman aralıklarında ve farklı dozlarda uygulanmış ve hücre canlılığı üzerindeki etkisi MTT testi ile belirlenmiştir. 24 saatte, 5 mM ve 10 mM dozlarda metformin uygulanan Hep3B hücrelerinde proliferasyonun istatistiksel olarak anlamlı bir şekilde azaldığı gözlenmiştir. Diğer zaman aralıklarında ve dozlarda hücre canlılığı üzerinde herhangi bir etki gözlenmemiştir.

Keywords

Karaciğer Kanseri, Metformin, Hep3B, Sitotoksisite

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