Novel oxidative stress biomarker in sepsis: dynamic thiol-disulfide homeostasis

Year 2020, Volume: 45 Issue: 1, 63 - 70, 31.03.2020

Betül Kozanhan , Ayşe İlksen Eğilmez Busra Tok Cekmecelioglu Mahmut Sami Tutar , Gulay Eren , Deniz Bilgi Özel Çiğdem Damla Deniz , Salim Neşelioğlu , Ozcan Erel

https://doi.org/10.17826/cumj.637127

Cited By: 2

Abstract

Purpose: The aim of this study was to investigate the relationship between serum dynamic thiol-disulfide homeostasis (TDH) and sepsis, in adult patients.
Materials and Methods: In this observational, prospective, case-control study patients (n=44) diagnosed with sepsis compared with ages and gender-matched healthy controls (n=44). Patients were divided into two subgroups (survivors and non-survivors) dependent variable being remaining alive on the 28th day after ICU admission at the follow-up period. TDH parameters measured using a novel automatic and spectrophotometric method and compared statistically.
Results: In patients with sepsis, native thiol, total thiol, and disulfide levels were lower than the control group, and this difference was statistically significant. TDH parameters between surviving and non-surviving patients were similar at the sepsis diagnosis. However, significant differences found in native and total thiol levels at follow-up via repeated measurement analyses on days 3 and 7 in surviving and non-surviving sub-groups.
Conclusion: This study showed that impairment in dynamic TDH in adults with sepsis may be related to negative outcomes. 

Keywords

Dynamic thiol-disulfide homeostasis, Sepsis

Sepsis için yeni bir oksidatif stres biyobelirteci: dinamik tiyol-disülfür homeostazisi

Abstract

Amaç: Bu gözlemsel, prospektif, vaka kontrol çalışmasında erişkin hastalarda serum dinamik Tiyol-Disülfir Homeostazisi (TDH) ile sepsis arasındaki ilişkiyi araştırmayı amaçladık.
Gereç ve Yöntem: Sepsis tanısı ile alan 44 hasta, yaş ve cinsiyet uyumlu 44 sağlıklı kontrol ile karşılaştırıldı. Hastalar yoğun bakım ünitesi yatışından sonraki 28. günde sağ-kalan ve hayatını kaybedenler olarak 2 gruba ayrıldı. TDH parametreleri yeni, otomatik ve spektrofotometrik bir yöntem kullanılarak ölçüldü ve sonuçlar istatistiksel olarak karşılaştırıldı.
Bulgular: Sepsisli hastalarda, nativ tiyol, total tiyol ve disülfit seviyeleri kontrol grubuna göre daha düşük olarak bulundu ve bu fark istatistiksel olarak anlamlı idi. Başlangıç TDH parametreleri sağ-kalan ve hayatını kaybeden hastalar arasındaki benzer bulundu. Ancak sağ kalan ve ex olan hastaların 3 ve 7. günde tekrarlanan TDH ölçümleri (nativ ve toplam tiyol seviyeleri) arasında anlamlı farklar bulundu.
Sonuç: Bu çalışma, erişkin sepsis hastalarında dinamik TDH'deki bozulmanın negatif sonuçlara ilişkili olabileceğini göstermiştir. 

Keywords

Dinamik Tiyol-Disülfir Homeostazisi; Sepsis; Oksidatif stres.

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