Investigation of uroprotective effects of seed methanol extracts of Hypericum triquetrifolium Turra. on cyclophosphamide-induced bladder hemorrhagic cystitis and nephrotoxicity in Wistar albino rats

Year 2020, Volume: 45 Issue: 4, 1361 - 1371, 27.12.2020

Songül Çetik Yıldız , Cumali Keskin , Varol Şahintürk , Adnan Ayhancı

https://doi.org/10.17826/cumj.730817

Cited By: 3

Abstract

Purpose: This study investigated the possible uroprotective effects of Hypericum triquetrifolium Turra. (HT) seed methanol extracts (25,50,100 mg/kg, i.p., for 6 days) against cyclophosphamide (CYP)-induced (150 mg/kg, single dose, i.p.) acute bladder hemorrhagic cystitis (HC) and nephrotoxicity in rats.
Materials and Methods: Wistar albino rats used in this study were divided into nine groups, each including seven rats. Group 1 (control) was treated with 0.5ml saline (SF) and Group 2 was treated with CYP (150 mg/kg). Groups 3, 4, 5 were treated with 25, 50, 100 mg/kg HT, respectively while groups 6, 7, 8 were treated with 25, 50, 100 mg/kg CYP + HT, respectively. Finally, Group 9 (control-2) was treated with 0.5ml-%0.2 dimethyl sulfoxide (DMSO). The serum creatinine, blood urea nitrogen (BUN), superoxide dismutase (SOD) and catalase (CAT) levels were measured in blood serum.
Results: The CYP-treated rats histopathologically had mild-moderate bladder and renal injuries. The serum creatinine and BUN levels, which are the biochemical markers of renal injury, significantly increased compared to the control group.
Conclusion: HT showed a protective effect on CYP-related bladder HC and nephrotoxicity in rats by inhibiting inflammation and apoptosis.

Keywords

cyclophosphamide

Supporting Institution

This study was supported by Mardin Artuklu University - Coordination Unit of Scientific Research Project (MAU-BAP-15-SHMYO-23).

Project Number

MAU-BAP-15-SHMYO-23

Wistar albino sıçanlarında Hypericum triquetrifolium Turra. tohum metanol ekstraktlarının siklofosfamid-nedenli mesane hemorajik sistiti ve nefrotoksisitesi üzerine üroprotektif etkilerinin incelenmesi

Abstract

Amaç: Bu çalışmada, Hypericum triquetrifolium Turra (HT) tohum metanol ekstraktlarının (25,50,100 mg/kg, i.p., 6 gün boyunca) siklofosfamid (CYP) nedenli (150 mg/kg, tek doz, i.p.) akut mesane hemorajik sistit (HC) ve nefrotoksisiteye karşı olası üroprotektif etkileri araştırıldı.
Gereç ve Yöntem: Bu çalışmada kullanılan Wistar albino sıçanlar, her biri yedi sıçan olmak üzere dokuz gruba ayrıldı. Grup 1 (kontrol) 0.5ml salin (SF) ile muamele edildi ve Grup 2, CYP (150 mg/kg) ile muamele edildi. Grup 3, 4, 5 sırasıyla 25, 50, 100 mg/kg HT ile tedavi edilirken, grup 6, 7, 8 sırasıyla 25, 50, 100 mg/kg HT + CYP ile tedavi edildi. Son olarak, Grup 9 (kontrol-2) 0.5ml-% 0.2 dimetil sülfoksit (DMSO) ile muamele edildi. Kan serumunda serum kreatinin, kan üre azotu (BUN), süperoksit dismutaz (SOD) ve katalaz (CAT) seviyeleri ölçüldü.
Bulgular: CYP ile tedavi edilen sıçanların histopatolojik olarak hafif-orta derecede mesane ve böbrek yaralanmaları vardı. Böbrek hasarının biyokimyasal belirteçleri olan serum kreatinin ve BUN düzeyleri, kontrol grubuna göre önemli ölçüde artmıştır.
Sonuç: HT, enflamasyonu ve apoptozu inhibe ederek sıçanlarda CYP ile ilişkili mesane HC'si ve nefrotoksisite üzerinde koruyucu bir etki göstermiştir.

Keywords

Siklofosfamid, Hypericum triquetrifolium, Nefrotoksiste, Hemorajik Sistit, Üroprotektif

Project Number

MAU-BAP-15-SHMYO-23

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