Oxidative stress markers in liver in streptozocin-induced diabetic rats: effects of metformin and sitagliptin

Year 2020, Volume: 45 Issue: 4, 1733 - 1741, 27.12.2020

Suat Çakına , Şamil Öztürk

https://doi.org/10.17826/cumj.791369

Cited By: 2

Abstract

Purpose: This study aims at investigating the oxidative stress effect of antidiabetic drugs of Metformin (MET) and sitagliptin (STG) in the liver tissue of diabetic rats from streptozotocin (STZ).
Materials and Methods: Thirty-five female Wistar rats (3-4 months old, weighing 200±25 g) were divided into five groups (with seven rats each) and treated as follows: control (Cont), streptozotocin alone (STZ), streptozotocin + metformin (STZ+MET), streptozotocin + sitagliptin (STZ+STG), streptozotocin + metformin + sitagliptin (STZ+MET+STG). Sitagliptin, Metformin, and combined metformin sitagliptin treated subgroups for four weeks. Malondialdehyde (MDA), total antioxidant status (TAS), total oxidant status (TOS) levels, and oxidative stress index (OSI) ratio were measured in rat liver tissue. Besides, cells undergoing apoptotic cell death were determined using the TUNEL technique through histopathological evaluation.
Results: MDA, TAS, and OSI of STZ+MET+STG administered group decreased compared to STZ. TOS of STZ+MET+STG administered group decreased compared to STZ.
Conclusion: In the experimental T2D model in rats, it shows protective effect when sitagliptin is used with metformin against oxidative damage in liver tissue caused by STZ.

Keywords

metformin, sitagliptin, oxidative stress, diabet

Streptozotosin kaynaklı diyabetik sıçanların karaciğerindeki oksidatif stres belirteçleri: metformin ve sitagliptinin etkileri

Abstract

Amaç: Bu çalışmanın amacı, streptozotosin (STZ) kaynaklı diyabetik sıçanların karaciğer dokusunda metformin (MET) ve/veya sitagliptinin (STG) antidiyabetik ilaçların olası oksidatif stres etkisini araştırmaktır.
Gereç ve Yöntem: Bu çalışmada erişkin dişi Wistar albino sıçanları kullanılmış ve rastgele kontrol normal grubu, kontrol diyabetik grubu ve diyabetik gruba ayrılmıştır. Antidiyabetik ilaç grubuna sahip diyabetik sıçanlar aşağıdaki tedavi edilen alt gruplara ayrıldı: Sitagliptin (10 mg/ kg/ gün), Metformin (200mg/ kg/ gün) ve Kombine metformin sitagliptin ile 4 hafta boyunca tedavi edilen alt gruplar. Sıçan karaciğer dokusunda oksidatif stres belirteçleri (Malondialdehid (MDA), total antioksidan durum (TAS), total oksidan durum (TOS) ve oksidatif stres indeksi (OSI) seviyeleri) ölçüldü. Ek olarak, apoptotik hücre ölümü geçiren hücreler histopatolojik değerlendirme ile TUNEL tekniği kullanılarak belirlendi.
Bulgular: STZ + MET + STG uygulanan grubun MDA, TAS ve OSI'si STZ'ye göre azaldı. STZ + MET + STG uygulanan grubun TOS'u STZ'ye göre azaldı.
Sonuç: Sıçanlarda deneysel T2D modelinde, sitagliptin metformin ile birlikte kullanıldığında karaciğer dokusunda STZ'nin neden olduğu oksidatif hasara karşı koruyucu etki göstermektedir.

Keywords

metformin, sitagliptin, oksidatif stres, diyabet

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