The ameliorative effects of melatonin on acetic acid-induced gastric ulcer in rats via its modulatory effects on gut microbiota

Year 2021, Volume: 46 Issue: 1, 186 - 200, 31.03.2021

Sevil Arabacı Tamer , Alper Yıldırım Ozge Cevik , Burak Aksu Meral Yüksel , Ekin Kuntsal Serap Sirvancı , Berrak Yeğen

Abstract

Purpose: The aim of this study was o observe the possible protective effects of melatonin pretreatment on oxidative damage and microbiota alteration due to gastric ulcer in rats.
Materials and Methods: Wistar-albino rats were given (n=32) melatonin (4 mg/kg/day), antibiotic mixture (AB; 1g/L ampicillin + 1g/L neomycin + 1g/L metronidazole), melatonin+AB in drinking water for 12 days or tap water for 15 days (control group; n=8). Subsequently, ulcer was induced. All treatments were continued for three days. Gastric tissues were obtained for biochemical and histopathological examinations, and fecal samples from the rectum were stored for bacteriological measurements.
Results: MPO and MDA levels were increased in untreated ulcer groups compared to the control group. In addition, the levels of luminol-lucigenin chemiluminescence (CL) and 8-OHdG and TNF-α and IL-8 protein expressions were also increased, while TNF-α, IL-8, MDA, 8-OHdG, luminol and lucigenin CL levels were significantly decreased in the melatonin-treated ulcer groups. However, melatonin+AB pretreatment increased antioxidant GSH levels and anti-inflammatory IL-10 levels, and suppressed caspase-3 activity and reduced MPO back to control level..
Conclusion: We anticipate that melatonin treatment, which is an effective antioxidant and radical scavenger, can accelerate ulcer healing along with antibiotics and increase the variety of bacteria impaired by antibiotics in the colon.

Melatoninin bağırsak mikrobiyotasını düzenleyici etkileri aracılığıyla sıçanlarda asetik asitle oluşturulmuş mide ülserinde hafifletici etkileri

Abstract

maç: Bu çalışmda melatonin ön-tedavisinin, sıçanlarda mide ülserine bağlı gerçekleşen mikrobiyota değişimine ve oksidatif hasara karşı koruyucu etkilerini araştırmak hedeflenmiştir.
Gereç ve Yöntem: Wistar albino sıçanlara (n=32) 12 gün boyunca içme suyu, melatonin (4mg/kg/gün), antibiyotik karışımı (AB; 1 g/L ampisilin + 1 g/L neomisin+ 1 g/L metronidazol), melatonin+ AB içme suyunda verildi. Ardından ülser oluşturuldu. Katkısız içme suyu alan sıçanların yarısına cerrahi işlem yapıldı ama asetik asit uygulanmadı (kontrol grubu; n=8). Cerrahi sonrasındaki 3 gün boyunca uygulanan tüm tedavilere devam edildi. Mide dokuları biyokimyasal ve histopatolojik incelemeler için çıkarıldı. Rektumdan alınan fekal örnekler bakteriyolojik ölçümler için uygun koşullarda saklandı.
Bulgular: Tedavi almayan ülser grubunun mide dokusunda MDA, MPO, 8-OHdG, luminol ve lusigenin kemilüminesans seviyeleri, TNF-α ve IL-8 protein ekspresyonları kontrol grubuna göre anlamlı derecede artarken, antioksidan GSH seviyesinin azaldığı gözlendi. Melatonin+AB ön-tedavi uygulaması antioksidan GSH seviyesini ve anti-inflamatuvar IL-10 miktarını artırmış, kaspaz-3 aktivitesini baskılamış ve dokuya nötrofil göçünü kontrol düzeyine inecek şekilde azaltmıştır.
Sonuç: Etkin bir antioksidan ve radikal süpürücü olan melatoninin antibiyotik ile birlikte verilmesinin, ülser iyileşmesini hızlandırmada ve kolondaki dağılımı bozulan bakteriyel çeşitliliği düzeltmede daha etkili olacağı öngörülmektedir.

Keywords

Mide ülseri, asetik asit, melatonin, mikrobiyota, inflamasyon, oksidatif stres

Supporting Institution

YOK

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