Antioxidant activities of inula viscosa extract and curcumin on U87 cells induced by beta-amyloid

Year 2021, Volume: 46 Issue: 2, 583 - 588, 30.06.2021

Ares Alizade , Gülüzar Özbolat

https://doi.org/10.17826/cumj.854213

Cited By: 1

Abstract

Purpose: The aim of this study was to investigate the effects of Inula viscosa extract and Curcumin on the U87 (human astrocytoma cell line) treated with amyloid-beta (Aβ), which is the Alzheimer’s disease (AD) model cell line.
Materials and Methods: Firstly, the cytotoxic potential of ınula and curcumin was investigated in the U87 cells by the colorimetric MTT (3-4,5-dimethyl-thiazolyl-2,5-diphenyltetrazolium bromide) assay. Then, the amount of Total Glutathione, Malondialdehyde (MDA), Glutathione reductase (GR) activities were investigated. ELISA test was used to examine the expression and activity of cleaved Bax and Bcl-2 proteins in the Inula viscosa and Curcumin treated U87 cell lines.
Results: Inula viscosa and Curcumin treatment reduced cell death caused by amyloid-B in cells. It also reduced the oxidative stress caused by amyloid-B, while reducing the activation of the proapoptotic protein Bax, and Bcl-2.
Conclusion: Our results suggest that inula viscosa may represent a new approach in the treatment of Alzheimer's.

Keywords

ınula viscosa, curcumin, Alzheimer, U87

Supporting Institution

Mustafa Kemal University

Project Number

17.M.003

Thanks

This study was funded by the Mustafa Kemal University Research Projects Unit (Project No.: 17.M.003).

Beta-amiloid ile indüklenen U87 hücrelerinde inula viscosa ekstresi ve kurkuminin antioksidan aktiviteleri

Abstract

Amaç: Bu çalışmada, Inula viscosa ekstresi ve Kurkumin'in Alzheimer hastalığı (AD) model hücre dizisi olan amiloid-beta (Aβ), ile tedavi edilen U87 (insan astrositom hücre dizisi) üzerindeki etkilerini araştırmayı amaçladık.
Gereç ve Yöntem: İlk olarak, U87 hücrelerinde inula ve kurkuminin sitotoksik potansiyeli kolorimetrik MTT (3-4,5-dimetil-tiyazolil-2,5-difeniltetrazolyum bromür) testi ile araştırıldı. Daha sonra Total Glutatyon miktarı, Malondialdehit (MDA), Glutatyon redüktaz (GR) aktiviteleri araştırıldı. Inula viscosa ve Curcumin ile muamele edilmiş U87 hücre hatlarında bölünmüş Bax ve Bcl-2 proteinlerinin ekspresyonunu ve aktivitesini incelemek için ELISA testi kullanıldı.
Bulgular: Inula viscosa ve Kurkumin muamelesinin, hücrelerde amiloid-B'nin neden olduğu hücre ölümünü azalttı. Ayrıca, proapoptotik protein Bax ve Bcl-2'nin aktivasyonunu azaltırken amiloid-B'nin neden olduğu oksidatif stresi de azalttı.
Sonuç: Sonuçlarımız, inula viscosa'nın Alzheimer tedavisinde yeni bir yaklaşımı temsil edebileceğini göstermektedir.

Keywords

ınula viscosa, curcumin, U87, Alzheimer

Project Number

17.M.003

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