Total antioksidan kapasite ve oksidatif stresin IVF siklusunda fertilizasyon ve embriyo seçimindeki rolü

Year 2021, Volume: 46 Issue: 4, 1694 - 1702, 30.12.2021

Sevtap Seyfettinoğlu , Gülnaz Şahin , Ayşin Akdoğan Ege Nazan Tavmergen Göker , Yasemin Akçay Eser Y. Sözmen , Erol Tavmergen

https://doi.org/10.17826/cumj.979955

Abstract

Amaç: Bu çalışmada IVF sikluslarında foliküler sıvı ve embriyo kültür ortamındaki oksidan ve antioksidan parametrelerin değerlendirilmesi ve embriyo kalitesi, fertilizasyon başarısı ve gebelik üzerine etkilerinin araştırılması amaçlanmıştır.
Gereç ve Yöntem: Bu prospektif çalışmaya IVF/ICSI uygulanan 51 hasta dahil edildi. Toplam 454 foliküler sıvıdan iki yüz altmış sıvı ve transfer edilen elli bir embriyonun (her hastadan bir tane) hücre kültürü ortamı incelendi. Antioksidan aktivite (AOA), Trolox eşdeğer antioksidan kapasite (TEAC), tiyobarbitürat reaktif maddeler (TBARS), ferrik antioksidan güç (FRAP), paraoksonaz, nitrotirozin analiz edildi.
Bulgular: TBARS, döllenmiş oositlerin foliküler sıvısında, döllenmemiş oositlere göre önemli ölçüde daha düşüktü (21'e karşı 29.75 nmol/ml,). Diğer parametreler açısından fark yoktu. TEAC seviyeleri, agonist protokol grubundaki oositlerde antagonist protokol grubuna göre daha yüksekti. Embriyo kalitesi açısından hiçbir analizde gruplar arasında fark yoktu. İkinci gün embriyo transferinden sonra gebe kalan grupta embriyo hücre kültürü ortamında TEAC düzeyleri daha yüksekti.
Sonuç: Hem folikül sıvısında hem de embriyo hücre kültürü ortamında oksidatif stres ve antioksidan parametreler değerlendirildiğinde lipid peroksidasyonunun fertilizasyonu olumsuz etkilediği ancak embriyo kalitesi ve gebelik oranları açısından önemli bir fark olmadığı saptanmıştır.

Keywords

IVF, oksidatif stres, embriyo kalitesi

Role of total antioxidant capacity and oxidative stress in fertilization and embryo selection in the IVF cycle

Abstract

Purpose: This study aims to evaluate the oxidant and antioxidant parameters in follicular fluid and embryo culture medium in IVF cycles and investigate their effects on embryo quality, fertilization success, and pregnancy.
Materials and Methods: Fifty-one patients who underwent IVF/ICSI were included in this prospective study. Two hundred sixty of a total of 454 follicular fluids and the cell culture media of transferred fifty-one embryos (one from each patient) were examined. Antioxidant activity (AOA), Trolox equivalent antioxidant capacity (TEAC), thiobarbiturate reactive substances (TBARS), ferric antioxidant power (FRAP), paraoxonase, nitrotyrosine were analyzed.
Results: TBARS were significantly lower in the follicular fluid of fertilized oocytes than in non-fertilized oocytes (21 vs. 29.75 nmol/ml). There was no difference in terms of other parameters. TEAC levels were higher in oocytes in the agonist protocol group than in the antagonist protocol group. In terms of embryo quality, there were no differences between the groups in any analysis. TEAC levels were higher in the embryo cell culture medium in the group that became pregnant after embryo transfer on the second day.
Conclusion: When oxidative stress and antioxidant parameters were evaluated in both follicle fluid and embryo cell culture medium, it was determined that lipid peroxidation negatively affected fertilization. However, there is no significant difference in terms of embryo quality and pregnancy rates.

Keywords

IVF, oxidative stres, embryo quality

References

• 1. Attaran M, Pasqualotto E, Falcone T, Goldberg JM, Miller KF, Agarwal A, Sharma RK. The effect of follicular fluid reactive oxygen species on the outcome of in vitro fertilization. Int. J. Fertil. Womens Med. 2000;45: 314-320.
• 2. Jozwik M, Wolczynski S, Jozwik M, Szamatowicz M. Oxidative stress markers in preovulatory follicular fluid in humans. Mol Hum Reprod 1999:5;409–413.
• 3. Sabatini L, Wilson C, Lower A, Al-Shawaf , Grudzinskas JG. Superoxide dismutase activity in human follicular fluid after controlled ovarian hyperstimulation in women undergoing invitro fertilization. Fertil Steril. 1999; 72: 1027-1034.
• 4. Esfandiari N, Falcone T, Agarwal A, Attaran M, Nelson DR, Sharma RK. Protein supplementation and the incidence of apoptosis and oxidative stress in mouse embryos. Obstet Gynecol. 2005;105:653–660.
• 5. Wang X, Falcone T, Attaran M, Goldberg JM, Agarwal A, Sharma RK.. Vitamin C and vitamin E supplementation reduce oxidative stress-induced embryo toxicity and improve the blastocyst development rate. Fertil Steril. 2002;78:1272–1277.
• 6. Das S, Chattopadhyay R, Ghosh S, Goswami SK, Chakravarty BN, Chaudhury K. Reactive oxygen species level in follicular fluid--embryo quality marker in IVF? Hum Reprod. 2006;21(9):2403-7
• 7. Veeck L (1990) Infertility. In Keel BA and Webster RW (eds) Handbook of Laboratory Diagnosis and Treatment. CRC Press, Boca Raton, FL, USA, pp. 353 369. 8. Oyawoye O, Abdel Gadir A, Garner A, Constantinovici N, Perrett C, Hardiman P. Antioxidants and reactive oxygen species in follicular fluid of women undergoing IVF: relationship to outcome. Hum Reprod 2003;18: 2270–2274.
• 9. Paszkowski T, Clarke RN. Antioxidant capacity of preimplantation embryo culture medium declines following the incubation of poor quality embryos. Hum. Reprod. 1996;11:2493-2495.
• 10. Yang HW, Hwang KJ, Kwon HC, Kim HS, Choi KW, Oh KS. Detection of reactive oxygen species (ROS) and apoptosis in human fragmented embryos. Hum Reprod. 1998;13(4):998-1002.
• 11. Hosseini SM, Forouzanfar M, Hajian M, Asgari V, et al. Antioxidant supplementation of culture medium during embryo development and/or after vitrification-warming; which is the most important? J Assist Reprod Genet. 2009;26(6):355-64.
• 12. Bedaiwy MA, Mahfouz RZ, Goldberg JM, Sharma R, Falcone T. Abdel Hafez MF, Agarwal A: Relationship of reactive oxygen species levels in day 3 culture media to the outcome of in vitro fertilization/intracytoplasmic sperm injection cycles. Fertil Steril. 2010; 94:2037–2042.
• 13. Guerin P, El Mouatassim S, Menezo Y. Oxidative stress and protection against reactive oxygen species in the pre-implantation embryo and its surroundings. Hum. Reprod. Update. 2001:7;175–189.
• 14. Orsi NM, Leese HJ. Protection against reactive oxygen species during mouse preimplantation embryo development: role of EDTA, oxygen tension, catalase, superoxide dismutase and pyruvate. Mol Reprod Dev. 2001;59: 44–53.
• 15. Spyropoulou I, Karamalegos C, Bolton VN. A prospective randomized study comparing the outcome of in-vitro fertilization and embryo transfer following culture of human embryos individually or in groups before embryo transfer on day 2. Hum Reprod. 1999 Jan;14(1):76-9.
• 16. Esterbauer H, Schaur RJ, Zollner H. Chemistry and biochemistry of 4-hydroxynonenal, malondialdehyde and related aldehydes. Free Rad. Biol. Med.1991;11:81–128. Review
• 17. Sabuncu T, Vural H, Harma M, Harma M. Oxidative stress in polycystic ovary syndrome and its contribution to the risk of cardiovascular disease. Clin Biochem. 2001;34:407–413.
• 18. Dursun P, Demirtas E, Bayrak A, Yarali H. Decreased serum paraoxonase 1 (PON1) activity: an additional risk factor for atherosclerotic heart disease in patients with PCOS? Hum Reprod 2006;21:104-108.
• 19. Hong-Liang L, De-Pei L, Chihj-Chuan L. Paraoxonase gene polymorphisms, oxidative stress and diseases. J Mol Med 2003; 81: 766-779.
• 20. Marsillach J, Checa MA, Pedro-Botet J, Carreras R, Joven J, Camps J. Paraoxonase-1 in female infertility: a possible role against oxidative stress-induced inflammation. Fertil Steril. 2010; 94(3):1132-1134.
• 21. Meijide S, Pérez-Ruiz I, Hernández ML, Navarro R, Ferrando M, Larreategui Z, Ruiz-Sanz JI, Ruiz-Larrea MB. Paraoxonase activities in human follicular fluid: role in follicular maturation. Reprod Biomed Online. 2017 Oct;35(4):351-362.
• 22. Barroso G, Barrionuevo M, Rao P, Graham L, Danforth D, Huey S, Abuhamad A, Oehninger S. Vascular endothelial growth factor, nitric oxide, and leptin follicular fluid levels correlate negatively with embryo quality in IVF patients. Fertil Steril. 1999 Dec;72(6):1024-6.
• 23. Battaglia C, Regnani G, Marsella T, Facchinetti F, Volpe A, Venturoli S, Flamigni C. Adjuvant L-arginine treatment in controlled ovarian hyperstimulation: a double-blind, randomized study. Hum Reprod. 2002; 17: 659-665.
• 24. Manau D, Balasch J, Jiménez W, Fábregues F, Civico S, Casamitjana R, Creus M, Vanrell JA. Follicular fluid concentrations of adrenomedullin, vascular endothelial growth factor and nitric oxide in IVF cycles: relationship to ovarian response. Hum Reprod. 2000; 15:1295–1299.
• 25. Salvolini E, Buldreghini E, Lucarini G, Vignini A, Di Primio R, Balercia G. Nitric oxide synthase and tyrosine nitration in idiopathic asthenozoospermia: an immunohistochemical study. Fertil Steril. 2012; 97(3):554-660.
• 26. Agarwal A, Durairajanayagam D, du Plessis SS. Utility of antioxidants during assisted reproductive techniques: an evidence based review. Reprod Biol Endocrinol. 2014 Nov 24;12:112. doi: 10.1186/1477-7827-12-112.