Daidzeinin ovaryum iskemi reperfüzyonu hasarındaki koruyucu etkisi

Year 2022, Volume: 47 Issue: 1, 102 - 110, 31.03.2022

Erdem Toktay , Muhammet Ali Gürbüz , Tuğba Bal , Özlem Özgül , Elif Erbaş , Rüstem Anıl Ugan , Jale Selli

https://doi.org/10.17826/cumj.993250

Cited By: 1

Abstract

Amaç: Bu çalışmada, güçlü antioksidan daidzeinin (DZ) over iskemi ve reperfüzyon hasarı üzerindeki potansiyel etkisini araştırdık.
Gereç ve Yöntem: Toplam 42 adet dişi Sprague-Dawley sıçan rastgele yedi gruba ayrıldı. Deney modeli için, 3saatlik iskeminin ardından klempler çıkarıldı ve tekrar kan akışı sağlandı. Reperfüzyon 3 saatin sonunda sonlandırıldı. Daidzein, iskemi (I) ve iskemi ve reperfüzyon (I/R) işlemlerinden 30 dakika önce hayvanlara ağızdan 35 ve 70 mg/kg dozlarında uygulandı.
Bulgular: I ve I/R gruplarında IL-1β, IL 6 ve Caspase 3'ün şiddetli immünoreaktivite görüldü. I+DZ35 ve I/R+DZ35 gruplarında IL-1β, IL 6 ve Kaspaz 3'ün immünreaktivitesi orta düzeyde iken, I+DZ70 ve I/R+DZ70 gruplarında hafif pozitiftir. Daidzein ile tedavi edilen gruplarda SOD aktivite seviyesi artarken, MDA seviyeleri azaldı. Ayrıca I/R + DZ70 ve IR+DZ35 gruplarında I/R grubuna göre doza bağlı olarak kanama alanları ve inflamatuar hücre göçü azaldı.
Sonuç: Daidzein, over iskemi-reperfüzyon hasarının tedavisinde güçlü bir koruyucu role sahiptir ve terapötik bir ajan olarak kullanılabilir.

Keywords

Daidzein, ovaryum, iskemisi ve reperfüzyon hasarı, sıçan

Protective effect of daidzein on ovarian ischemia‑reperfusion injury in rats

Abstract

Purpose: In this study, our aim was to investigate the potential effects of strong antioxidant daidzein (DZ) on ovarian ischemia and reperfusion injury.
Materials and Methods: A total of 42 female Sprague-Dawley rats were randomly divided into seven groups. For the experimental model, the clamps were removed after 3 hours of ischemia, and blood flow was provided again. Then, reperfusion process was terminated for 3 hours. Daidzein was orally administered to animals at doses of 35 and 70 mg/kg 30 minutes before ischemia (I) and ischemia and reperfusion (I/R) procedures.
Results: Severe immunoreactivity of the IL-1β, IL-6 and Caspase-3 were detected in I and I/R groups. Moderate immunoreactivity of IL-1β, IL-6 and Caspase-3 was detected in I+DZ35 and I/R+DZ35 groups, and slightly positivity was detected in I+DZ70 and I/R+DZ70 groups. The SOD activity level increased in the groups treated with Daidzein, while MDA levels decreased. In addition, hemorrhage areas and inflammatory cell migration decreased in I/R+DZ70 and I/R+DZ35 groups, when compared to I/R group in a dose dependent manner.
Conclusion: Daidzein has a strong protective role in the treatment of ovarian ischemia-reperfusion injury and can be used as a therapeutic agent.

Keywords

Daidzein, ovary, ischemia‑reperfusion injury, rat

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