DNA damage and inflammation in COVID-19 cases

Year 2022, Volume: 47 Issue: 3, 1073 - 1079, 30.09.2022

Gonca Gülbay , Atakan Savrun

https://doi.org/10.17826/cumj.1102599

Abstract

Purpose: The aim of this study is to see oxidative DNA damage (8-OHdG), its relationship with inflammatory mediators (IL6 and TNFA), and its reflections on laboratory findings in patients who had COVID-19 infection at different intensities.
Materials and Methods: Serum interleukin-6 (IL6), tumor necrosis factor-alpha (TNFA), and 8-hydroxy-2′-deoxyguanosine (8-OHdG) levels were measured using kits based on the enzyme-linked immunosorbent assay (ELISA) principle.
Results: In COVID-19 positive patients treated in intensive care 8-OHdG marker level is at the highest level and statistically significant. In patients receiving inpatient treatment in the hospitalized, the 8-OHdG marker level is higher than the control and outpatient groups. IL6 values were at the highest level in the patient group treated in the intensive care unit and were higher than the outpatient and control groups. There was no statistically significant difference between the control and patient groups in terms of TNFA values. Neutrophil-to-lymphocyte ratio (NLR) was lower in the control group than in all patient groups. C-reactive protein (CRP) is higher in hospitalized patients than in the control group. Lactate dehydrogenase (LDH) was found to be statistically significantly higher in hospitalized patients than outpatients.
Conclusion: As the severity of COVID-19 increases, serum 8-OHdG and IL6 levels also increase. These parameters can guide the diagnosis of COVID-19 patients in the early stages of the disease course.

Keywords

COVID-19, DNA damage, Interleukin-6, Tumor necrosis factor-alpha, 8-hydroxy-2′-deoxyguanosine

Supporting Institution

Ordu University Scientific Research Projects Coordination Unit

Project Number

A-2119

Thanks

1. Statistical analysis of our study were made by Assistant Professor Yeliz Kasko ARICI, Department of Biostatistics and Medical Informatics, Faculty of Medicine, Ordu University. 2. This research was supported by Ordu University Scientific Research Projects Coordination Unit (Project number: A-2119) Ordu, Turkey

COVID-19 vakalarında DNA hasarı ve enflamasyon

Abstract

Amaç: Çalışmamızda, COVID-19 enfeksiyonunu farklı şiddetlerde geçiren hastalarda oksidatif DNA hasarını (8-OHdG), inflamatuar mediatörlerle (IL6 ve TNFA) ilişkisini, ve laboratuvar bulgularına yansımalarını görmeyi amaçladık.
Gereç ve Yöntem: Serum interlökin-6 (IL6), tümör nekroz faktör-alfa (TNFA) ve 8-hidroksi-2′-deoksiguanozin (8-OHdG) düzeyleri ELISA (enzyme-linked immunosorbent assay) prensibine dayalı kitler kullanılarak ölçülmüştür.
Bulgular: Yoğun bakımda tedavi edilen COVID-19 pozitif hastalarda 8-OHdG marker seviyesi en üst düzeyde ve istatistiksel olarak anlamlı idi. Yatarak tedavi gören hastalarda 8-OHdG marker düzeyi kontrol ve ayakta tedavi gruplarına göre daha yüksekti. Yoğun bakımda tedavi edilen hasta grubunda IL6 değerleri en yüksek düzeyde olup, ayaktan ve kontrol gruplarına göre daha yüksekti. TNFA değerleri açısından kontrol ve hasta grupları arasında istatistiksel olarak anlamlı fark yoktu. Nötrofil-lenfosit oranı (NLR) kontrol grubunda tüm hasta gruplarına göre daha düşüktü. Hastanede yatan hastalarda kontrol grubuna göre C-reaktif protein (CRP) daha yüksekti. Laktat dehidrojenaz (LDH) hastanede yatan hastalarda ayaktan hastalara göre istatistiksel olarak anlamlı derecede yüksek bulundu.
Sonuç: COVID-19' un şiddeti arttıkça serum 8-OHdG ve IL6 seviyeleri de artmaktadır. Bu parametreler COVID-19 hastalarının hastalık seyrinin erken evrelerinde tanınmasına rehberlik edebilir.

Keywords

Covid-19, DNA damage, Interleukin-6, Tumor necrosis factor-alpha, 8-hydroxy-2′-deoxyguanosine.

Project Number

A-2119

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